Human CXCL4/PF4 Antibody Summary
Accession # P02776
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CXCL4/PF4 by Western Blot. Western blot shows lysates of human spleen tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CXCL4/PF4 by Simple WesternTM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL4/PF4 at approximately 15 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) followed by 1:50 dilution of HRP-conjugated Anti-Goat Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL4, also known as Platelet Factor 4, is a member of the CXC chemokine family, CXCL4. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Human and mouse CXCL4 share a 64% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
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- Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
- Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Citations for Human CXCL4/PF4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Direct chemical reprogramming of human cord blood erythroblasts to induced megakaryocytes that produce platelets
Authors: J Qin, J Zhang, J Jiang, B Zhang, J Li, X Lin, S Wang, M Zhu, Z Fan, Y Lv, L He, L Chen, W Yue, Y Li, X Pei
Cell Stem Cell, 2022;29(8):1229-1245.e7.
Sample Types: Whole Cells
Applications: Flow Cytometry
Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
Redox Biology, 2021;48(0):102205.
Sample Types: Cell Culture Supernates
Applications: Dot Blot
Up-regulation of platelet activation in hemophilia A.
Authors: van Bladel ER, Roest M
Sample Types: Plasma
Applications: ELISA Development
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