CXCL4, also known as Platelet Factor 4, is a member of the CXC chemokine family, CXCL4. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Human and mouse CXCL4 share a 64% identity. CXCL4 contains several heparin-binding sites at the C‑terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
Human CXCL4/PF4 Antibody (170131)
R&D Systems | Catalog # MAB7951
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry, Immunocytochemistry, ELISA Capture, ELISA Development, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 170131
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Product Specifications
Immunogen
E. coli-derived recombinant human CXCL4/PF4
Glu32-Ser101
Accession # P02776.2
Glu32-Ser101
Accession # P02776.2
Specificity
Detects human CXCL4/PF4 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL1, rhCXCL2, or rmCXCL4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CXCL4/PF4 Antibody (170131)
Human CXCL4 / PF4 ELISA Standard Curve
Recombinant Human CXCL4/PF4 (Catalog # 795-P4) was serially diluted and captured by Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7951) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of CXCL4/PF4 by Western Blot
The N-terminus of CXCL8 is a key determinant of susceptibility to SpyCEP.A, cartoon showing CXCL4 and CXCL8-based chimeras with exchanged N-termini, together with the parent molecule CXCL4. B, alignment of wild type and chimeric chemokine sequences. C and D, cleavage products following incubation of WT CXCL8 and chimera 4:8 with SpyCEP for 16 h as imaged by multiplex Western blot (n = 3). E, quantification of WT CXCL8 and chimera 4:8 cleavage by CXCL8 ELISA (n = 6). F and G, cleavage products following incubation of WT CXCL4 and chimera 8:4 with SpyCEP for 16 h, as imaged by monoplex Western blot (n = 3). H, quantification of WT CXCL4 and chimera 8:4 by CXCL4 ELISA (n = 6). I and J, cleavage products following incubation of untagged and HA-tagged variants of WT CXCL8, WT CXCL4, and chimera 8:4 with SpyCEP for 16 h as resolved by SDS-PAGE and imaged by PAGE Blue staining (n = 3). K, quantification of CXCL8-HA and chimera 8:4-HA cleavage by Western blot with an anti-HA antibody (n = 3). Western blot data panels are representative of the indicated number of experiments. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S0021925825002972), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCL4/PF4 by Western Blot
The N-terminus of CXCL8 is a key determinant of susceptibility to SpyCEP.A, cartoon showing CXCL4 and CXCL8-based chimeras with exchanged N-termini, together with the parent molecule CXCL4. B, alignment of wild type and chimeric chemokine sequences. C and D, cleavage products following incubation of WT CXCL8 and chimera 4:8 with SpyCEP for 16 h as imaged by multiplex Western blot (n = 3). E, quantification of WT CXCL8 and chimera 4:8 cleavage by CXCL8 ELISA (n = 6). F and G, cleavage products following incubation of WT CXCL4 and chimera 8:4 with SpyCEP for 16 h, as imaged by monoplex Western blot (n = 3). H, quantification of WT CXCL4 and chimera 8:4 by CXCL4 ELISA (n = 6). I and J, cleavage products following incubation of untagged and HA-tagged variants of WT CXCL8, WT CXCL4, and chimera 8:4 with SpyCEP for 16 h as resolved by SDS-PAGE and imaged by PAGE Blue staining (n = 3). K, quantification of CXCL8-HA and chimera 8:4-HA cleavage by Western blot with an anti-HA antibody (n = 3). Western blot data panels are representative of the indicated number of experiments. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S0021925825002972), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human CXCL4/PF4 Antibody (170131)
Human CXCL4/PF4 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using MAB7951 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CXCL4/PF4
References
- Poncz, M. et al. (1987) Blood 69:219.
- Scheuerer, B. et al. (2000) Blood 95:1158.
- Perollet, C. et al. (1998) Blood 91:3289.
- Petersen, F. et al. (1998) J. Immunol. 161:4347.
- Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
- Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Alternate Names
PF4
Gene Symbol
PF4
UniProt
Additional CXCL4/PF4 Products
Product Documents for Human CXCL4/PF4 Antibody (170131)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CXCL4/PF4 Antibody (170131)
For research use only
Related Research Areas
Citations for Human CXCL4/PF4 Antibody (170131)
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/17/2017This antibody was used as the capture with BAF795 as the detection in an ELISA. While functional it was difficult to get a broad dynamic range on the ELISA with this antibody.
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