Human CXCL9/MIG DuoSet ELISA

Catalog # Availability Size / Price Qty
DY392
DY392-05
Ancillary Products Available
Human CXCL9 / MIG ELISA Standard Curve
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Product Details
Procedure
Citations (18)
FAQs
Supplemental Products
Reviews

Human CXCL9/MIG DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CXCL9/MIG. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Human CXCL9 / MIG ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL9/MIG

CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.

Entrez Gene IDs:
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names:
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human CXCL9/MIG DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. The interferon-gamma pathway is selectively up-regulated in the liver of patients with secondary hemophagocytic lymphohistiocytosis
    Authors: G Prencipe, C Bracaglia, I Caiello, A Pascarella, P Francalanc, M Pardeo, A Meneghel, G Martini, MN Rossi, A Insalaco, G Marucci, V Nobili, M Spada, F Zulian, F De Benedet
    PLoS ONE, 2019;14(12):e0226043.
    Species: Human
    Sample Types: Plasma
  2. Identification of an immunogenic neo-epitope encoded by mouse sarcoma using CXCR3 ligand mRNAs as sensors
    Authors: K Fujii, Y Miyahara, N Harada, D Muraoka, M Komura, R Yamaguchi, H Yagita, J Nakamura, S Sugino, S Okumura, S Imoto, S Miyano, H Shiku
    Oncoimmunology, 2017;6(5):e1306617.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity
    Authors: JP Strassner, M Rashighi, M Ahmed Refa, JM Richmond, JE Harris
    J. Am. Acad. Dermatol, 2017;0(0):.
    Species: Human
    Sample Types: Blister Fluid
  4. IL-27 Modulates Chemokine Production in TNF-? -Stimulated Human Oral Epithelial Cells
    Authors: Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo
    Cell. Physiol. Biochem., 2017;43(3):1198-1206.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. CXCR3 mediates ascites-directed tumor cell migration and predicts poor outcome in ovarian cancer patients
    Authors: C Windmüller, D Zech, S Avril, M Boxberg, T Dawidek, B Schmalfeld, M Schmitt, M Kiechle, H Bronger
    Oncogenesis, 2017;6(5):e331.
    Species: Human
    Sample Types: Cell Lysates
  6. PRC2 Epigenetically Silences Th1-Type Chemokines to Suppress Effector T-Cell Trafficking in Colon Cancer.
    Authors: Nagarsheth N, Peng D, Kryczek I, Wu K, Li W, Zhao E, Zhao L, Wei S, Frankel T, Vatan L, Szeliga W, Dou Y, Owens S, Marquez V, Tao K, Huang E, Wang G, Zou W
    Cancer Res, 2016;76(2):275-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. CXCL9 and CXCL10 predict survival and are regulated by cyclooxygenase inhibition in advanced serous ovarian cancer
    Br J Cancer, 2016;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  8. Effect of JAK Inhibitors on Release of CXCL9, CXCL10 and CXCL11 from Human Airway Epithelial Cells.
    Authors: Fenwick P, Macedo P, Kilty I, Barnes P, Donnelly L
    PLoS ONE, 2015;10(6):e0128757.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014;5(0):4092.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. CXCR3 axis in patients with primary biliary cirrhosis: a possible novel mechanism of the effect of ursodeoxycholic acid.
    Authors: Manousou P, Kolios G, Drygiannakis I, Koulentaki M, Pyrovolaki K, Voumvouraki A, Notas G, Bourikas L, Papadaki H, Kouroumalis E
    Clin Exp Immunol, 2013;172(1):9-15.
    Species: Human
    Sample Types: Serum
  11. Abnormalities in serum chemokine levels in euthymic patients with bipolar disorder.
    Authors: Brietzke E, Kauer-Sant'Anna M, Teixeira AL, Kapczinski F
    Brain Behav. Immun., 2009;23(8):1079-82.
    Species: Human
    Sample Types: Serum
  12. Increased serum levels of CCL11/eotaxin in schizophrenia.
    Authors: Teixeira AL, Reis HJ, Nicolato R, Brito-Melo G, Correa H, Teixeira MM, Romano-Silva MA
    Prog. Neuropsychopharmacol. Biol. Psychiatry, 2008;32(3):710-4.
    Species: Human
    Sample Types: Serum
  13. The role of IkappaB kinase 2, but not activation of NF-kappaB, in the release of CXCR3 ligands from IFN-gamma-stimulated human bronchial epithelial cells.
    Authors: Tudhope SJ, Catley MC, Fenwick PS, Russell RE, Rumsey WL, Newton R, Barnes PJ, Donnelly LE
    J. Immunol., 2007;179(9):6237-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Modification of TLR-induced activation of human dendritic cells by type I IFN: synergistic interaction with TLR4 but not TLR3 agonists.
    Authors: Walker J, Tough DF
    Eur. J. Immunol., 2006;36(7):1827-36.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production.
    Authors: Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A
    J. Allergy Clin. Immunol., 2006;118(2):354-60.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Prediction of acute renal allograft rejection by urinary monokine induced by IFN-gamma (MIG).
    Authors: Hauser IA, Spiegler S, Kiss E, Gauer S, Sichler O, Scheuermann EH, Ackermann H, Pfeilschifter JM, Geiger H, Grone HJ, Radeke HH
    J. Am. Soc. Nephrol., 2005;16(6):1849-58.
    Species: Human
    Sample Types: Urine
  17. CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow.
    Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH
    Am. J. Pathol., 2005;167(3):887-99.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Chemokines and chemokine receptors in inflammatory demyelinating neuropathies: a central role for IP-10.
    Authors: Kieseier BC, Tani M, Mahad D, Oka N, Ho T, Woodroofe N, Griffin JW, Toyka KV, Ransohoff RM, Hartung HP
    Brain, 2002;125(0):823-34.
    Species: Human
    Sample Types: CSF

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