Human CXCL9/MIG Quantikine ELISA Kit

  (25 citations)     
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Assay Procedure
Citations (25)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    11.3 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human MIG
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC123 , Quantikine Immunoassay Control Set 860 for Human CXCL9/MIG - Please Inquire
Product Summary
The Quantikine Human MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MIG in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MIG.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 235 700 1358 242 707 1391
Standard Deviation 9.5 25.1 24.3 20.8 50.9 83.6
CV% 4 3.6 1.8 8.6 7.2 6

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 253 817 1603 245 723 1427
Standard Deviation 9.9 26.6 49.6 12.8 59.4 88.1
CV% 3.9 3.3 3.1 5.2 8.2 6.2

Recovery

The recovery of MIG spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 86-98
EDTA Plasma (n=5) 100 95-110
Heparin Plasma (n=5) 109 101-114
Serum (n=5) 108 101-113
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of MIG were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 CXCL9/MIG [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL9/MIG
MIG, a member of the CXC subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. MIG is a chemoattractant for activated T cells and TIL, but not for neutrophils or monocytes.
  • Entrez Gene IDs:
    4283 (Human); 17329 (Mouse); 246759 (Rat)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Species
Sample Type
  1. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017;6(5):.
    Species: Human
    Sample Type: Plasma
  2. Salt suppresses IFN? inducible chemokines through the IFN?-JAK1-STAT1 signaling pathway in proximal tubular cells
    Authors: Y Arai, D Takahashi, K Asano, M Tanaka, M Oda, SBH Ko, MSH Ko, S Mandai, N Nomura, T Rai, S Uchida, E Sohara
    Sci Rep, 2017;7(0):46580.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Left Ventricular Dysfunction and CXCR3 Ligands in Hypertension: From Animal Experiments to a Population-Based Pilot Study.
    Authors: Altara R, Gu Y, Struijker-Boudier H, Thijs L, Staessen J, Blankesteijn W
    PLoS ONE, 2015;10(10):e0141394.
    Species: Human
    Sample Type: Plasma
  4. Serum CXCL9 levels are associated with tumor progression and treatment outcome in patients with nasopharyngeal carcinoma.
    Authors: Hsin L, Kao H, Chen I, Tsang N, Hsu C, Liu S, Chang Y, Chang K
    PLoS ONE, 2013;8(11):e80052.
    Species: Human
    Sample Type: Serum
  5. BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy.
    Authors: Pranzatelli, Michael, Tate, Elizabet, McGee, Nathan R, Travelstead, Anna L, Colliver, Jerry A, Ness, Jayne M, Ransohoff, Richard
    J Neuroinflammation, 2013;10(0):10.
    Species: Human
    Sample Type: Serum
  6. Expression of CXCR3 and its ligands CXCL9, -10 and -11 in paediatric opsoclonus-myoclonus syndrome.
    Authors: Pranzatelli M, Tate E, McGee N, Travelstead A, Verhulst S, Ransohoff R
    Clin Exp Immunol, 2013;172(3):427-36.
    Species: Human
    Sample Type: Serum
  7. Increased CXCL9 serum levels in hepatitis C-related mixed cryoglobulinemia, with autoimmune thyroiditis, associated with high levels of CXCL10.
    Authors: Antonelli A, Fallahi P, Ferrari S, Colaci M, Giuggioli D, Saraceno G, Benvenga S, Ferri C
    J Interferon Cytokine Res, 2013;33(12):739-45.
    Species: Human
    Sample Type: Serum
  8. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
    Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
    PLoS Pathog., 2011;7(4):e1002016.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Circulating chemokine (CXC motif) ligand (CXCL)9 is increased in aggressive chronic autoimmune thyroiditis, in association with CXCL10.
    Authors: Antonelli A, Ferrari SM, Frascerra S, Galetta F, Franzoni F, Corrado A, Miccoli M, Benvenga S, Paolicchi A, Ferrannini E, Fallahi P
    Cytokine, 2011;55(2):288-93.
    Species: Human
    Sample Type: Serum
  10. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
    Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50.
    Species: Human
    Sample Type: Tears
  11. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
    Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
    Mediators Inflamm., 2009;2009(0):269541.
    Species: Human
    Sample Type: Serum
  12. CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
    Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA
    Eur. Respir. J., 2009;34(3):676-86.
    Species: Human
    Sample Type: BALF
  13. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009;182(6):3919-27.
    Species: Human
    Sample Type: Plasma
  14. Urinary fractalkine is a marker of acute rejection.
    Authors: Peng W, Chen J, Jiang Y, Wu J, Shou Z, He Q, Wang Y, Chen Y, Wang H
    Kidney Int., 2008;74(11):1454-60.
    Species: Human
    Sample Type: Urine
  15. The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma.
    Authors: Linge HM, Collin M, Giwercman A, Malm J, Bjartell A, Egesten A
    J. Interferon Cytokine Res., 2008;28(3):191-6.
    Species: Human
    Sample Type: Seminal Plasma
  16. Prolactin enhances interferon-gamma-induced production of CXC ligand 9 (CXCL9), CXCL10, and CXCL11 in human keratinocytes.
    Authors: Kanda N, Watanabe S
    Endocrinology, 2007;148(5):2317-25.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. Serum interleukin-18 and soluble tumour necrosis factor receptor 2 are associated with disease severity in patients with paracoccidioidomycosis.
    Authors: Corvino CL, Mamoni RL, Fagundes GZ, Blotta MH
    Clin. Exp. Immunol., 2007;147(3):483-90.
    Species: Human
    Sample Type: Serum
  18. Neuroinvasion of fluorescein-positive monocytes in acute simian immunodeficiency virus infection.
    Authors: Clay CC, Rodrigues DS, Ho YS, Fallert BA, Janatpour K, Reinhart TA, Esser U
    J. Virol., 2007;81(21):12040-8.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Plasma
  19. CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
    Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
    Chest, 2007;133(1):26-33.
    Species: Human
    Sample Type: Saliva
  20. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis.
    Authors: Barrera L, Mendoza F, Zuniga J, Estrada A, Zamora AC, Melendro EI, Ramirez R, Pardo A, Selman M
    Am. J. Respir. Crit. Care Med., 2007;177(1):44-55.
    Species: Human
    Sample Type: BALF
  21. Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
    Authors: Matsuda A, Fukuda S, Matsumoto K, Saito H
    Am. J. Respir. Cell Mol. Biol., 2007;38(2):168-75.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
    Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
    J. Immunol., 2007;178(2):1172-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
    Authors: Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM
    Chest, 2006;130(4):982-8.
    Species: Human
    Sample Type: BALF
  24. Modulation of cytokine secretion by pentacyclic triterpenes from olive pomace oil in human mononuclear cells.
    Authors: Marquez-Martin A, De La Puerta R, Fernandez-Arche A, Ruiz-Gutierrez V, Yaqoob P
    Cytokine, 2006;36(5):211-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  25. CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
    Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
    Transplantation, 2004;78(9):1341-50.
    Species: Human
    Sample Type: Urine
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Quantikine Immunoassay Control Set 860 for Human CXCL9/MIG

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