Best Seller

Human CXCL9/MIG Quantikine ELISA Kit

R&D Systems | Catalog # DCX900

R&D Systems
100% Guarantee

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)

Sensitivity

11.3 pg/mL

Assay Range

31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
View all CXCL9/MIG ELISA Kits »

Product Summary for Human CXCL9/MIG Quantikine ELISA Kit

The Quantikine Human MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MIG in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MIG.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human MIG

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human MIG in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=40)64.465ND-199
EDTA plasma (n=23)56.762ND-179
Heparin plasma (n=25)62.068ND-209
ND=Non-detectable

Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of natural human MIG.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
UnstimulatedND445
Stimulated77.512,290
ND=Non-detectable


Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 235 700 1358 242 707 1391
Standard Deviation 9.5 25.1 24.3 20.8 50.9 83.6
CV% 4.0 3.6 1.8 8.6 7.2 6.0

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 253 817 1603 245 723 1427
Standard Deviation 9.9 26.6 49.6 12.8 59.4 88.1
CV% 3.9 3.3 3.1 5.2 8.2 6.2

Recovery for Human CXCL9/MIG Quantikine ELISA Kit

The recovery of MIG spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 86-98
EDTA Plasma (n=5) 100 95-110
Heparin Plasma (n=5) 109 101-114
Serum (n=5) 108 101-113

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of MIG were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CXCL9/MIG ELISA Linearity

Scientific Data Images for Human CXCL9/MIG Quantikine ELISA Kit

Human CXCL9/MIG ELISA Cell Culture Supernate Standard Curve

Human CXCL9/MIG ELISA Cell Culture Supernate Standard Curve

Human CXCL9/MIG ELISA Serum/Plasma Standard Curve

Human CXCL9/MIG ELISA Serum/Plasma Standard Curve

Human CXCL9/MIG Quantikine ELISA Kit

Human CXCL9/MIG Quantikine ELISA Kit

Serum ELISA results of three protein biomarkers in renal transplantation.We measured the protein concentration of ten genes by ELISA in independent serum samples of 19 AR patients and 20 patients with stable (STA) graft function after renal transplant. The protein concentrations of PECAM1 (A), CXCL9 (B), and CD44 (C) were higher in the AR serum samples, as shown in the notched boxplots. When the notches about two medians do not overlap, the medians are roughly significantly different at about a 95% confidence level [54]. The circles are outliers. P-values were calculated using the Mann-Whitney U test. One of the AR samples was inadvertently lost during the PECAM1 experiment and could not be recovered. (D) Areas under the Receiver Operating Characteristic (ROC) curves used to distinguish AR from STA were 0.811, 0.864, and 0.761 for PECAM1, CXCL9, and CD44, respectively. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pcbi.1000940), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL9/MIG

CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.

Alternate Names

MIG

Entrez Gene IDs

4283 (Human); 17329 (Mouse); 246759 (Rat)

Gene Symbol

CXCL9

Additional CXCL9/MIG Products

Product Documents for Human CXCL9/MIG Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCL9/MIG Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CXCL9/MIG Quantikine ELISA Kit

Customer Reviews for Human CXCL9/MIG Quantikine ELISA Kit (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CXCL9/MIG Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Human CXCL9/MIG Quantikine ELISA Kit
    Name: Mike Ehrhardt
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 11/24/2020
    Human CXCL9/MIG Quantikine ELISA Kit DCX900
  • Human CXCL9/MIG Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Verified Customer | Posted 11/09/2020
    PBMCs from healthy donors stimulated with IFNg for 16h. Samples diluted 1:2.
    Human CXCL9/MIG Quantikine ELISA Kit DCX900
  • Human CXCL9/MIG Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 11/07/2019
    Human CXCL9/MIG Quantikine ELISA Kit DCX900

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

View specific protocols for Human CXCL9/MIG Quantikine ELISA Kit (DCX900):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits