Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 44717
View all CXCR4 Primary Antibodies »

Product Specifications

Immunogen

Mouse 3T3 cells transfected with human CXCR4

Specificity

Reacts specifically with human and non-human cells expressing human CXCR4 (fusin) as detected by flow cytometry. It will also react with cells expressing feline CXCR4 but not rat CXCR4. This antibody does not cross-react with other chemokine receptors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CXCR4 PE‑conjugated Antibody

Detection of CXCR4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 PE-conjugated Monoclonal Antibody (Catalog # FAB173P) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) for 15 minutes at 37°C followed by 15 minutes at room temperature. View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR4 by Flow Cytometry

Detection of CXCR4 by Flow Cytometry

Representative example of flow cytometry quantification of complexes neutrophil–CD41a+ (NPAs, neutrophil–platelet aggregates) and lymphocyte markers’ expression. (A) Neutrophil cluster identification (region R1) based on its low CD14 expression (FL3) and side-scattering (SSC) morphological characteristics. (B) The R1-based histogram’s subtraction analysis (positive events (continuous line) minus isotype control (dotted line)) was used to quantify both the percentage of complexes CD41a+ (grey events in M1) and their RFI (median of M1 histogram minus median of the isotype control). (C) Lymphocyte cluster identification based on its forward- (FSC) and side-scattering characteristics (region R1). (D) Representative quantification of lymphocyte CXCR4 expression following the overlay histogram’s subtraction analysis (see above). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36836785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCR4 by Flow Cytometry

Detection of CXCR4 by Flow Cytometry

Representative example of flow cytometry quantification of complexes neutrophil–CD41a+ (NPAs, neutrophil–platelet aggregates) and lymphocyte markers’ expression. (A) Neutrophil cluster identification (region R1) based on its low CD14 expression (FL3) and side-scattering (SSC) morphological characteristics. (B) The R1-based histogram’s subtraction analysis (positive events (continuous line) minus isotype control (dotted line)) was used to quantify both the percentage of complexes CD41a+ (grey events in M1) and their RFI (median of M1 histogram minus median of the isotype control). (C) Lymphocyte cluster identification based on its forward- (FSC) and side-scattering characteristics (region R1). (D) Representative quantification of lymphocyte CXCR4 expression following the overlay histogram’s subtraction analysis (see above). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36836785), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR4 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes

Reviewed Applications

Read 1 review rated 5 using FAB173P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR4

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

Gene Symbol

CXCR4

Additional CXCR4 Products

Product Documents for Human CXCR4 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CXCR4 PE‑conjugated Antibody

For research use only

Citations for Human CXCR4 PE‑conjugated Antibody

Customer Reviews for Human CXCR4 PE‑conjugated Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CXCR4 PE‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: induced pluripotent stem cells and endoderm
    Species: Human
    Verified Customer | Posted 12/15/2016
    We use CXCR4 to test the % positive definitive endoderm made from human induced pluripotent stem cells.

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies