Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

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Human PD-L1/B7-H1 ELISA Cell Culture Supernate/Cell Lysate/Serum/Plasma Standard Curve
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Citations (19)
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Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Cell Lysates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
Sensitivity
4.52 pg/mL
Assay Range
25.0 - 1,600 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human B7-H1/PD-L1. This assay also recognizes natural cynomolgus monkey B7-H1/PD-L1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Sample TypeMean (pg/mL)Range (pg/mL)
Human Serum (n=36)62.544.5-106
Cynomolgus Monkey Serum (n=10)461248-953
Human EDTA plasma (n=36)56.239.1-108
Cynomolgus monkey EDTA plasma (n=10)20289.1-497
Human heparin plasma (n=36)52.233.4-110
Sample TypeMean of Detectable (pg/mL)Range (pg/mL)
Human Urine (n=10)34.5ND-34.5
ND=Non-detectable

Product Summary

The Quantikine® Human/Cynomolgus Monkey B7-H1/PD-L1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human/cynomolgus monkey B7-H1 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains HEK293-expressed recombinant human B7-H1/PD-L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/cynomolgus monkey B7-H1/PD-L1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human/cynomolgus monkey B7-H1/PD-L1.
Read this White Paper on detecting B7-H1/PD-L1 levels in multiple cancer subtypes.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3
n 20 20 20
Mean (pg/mL) 244 466 878
Standard Deviation 15.9 25.8 66.4
CV% 6.5 5.5 7.6

Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 257 490 934 249 469 898
Standard Deviation 3.75 7.01 12.7 11.1 21.7 37.7
CV% 1.5 1.4 1.4 4.5 4.6 4.2

Recovery

The recovery of human/cynomolgus monkey B7-H1/PD-L1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 97-112
DYC002 (1X) (n=1) 96 90-100
EDTA Plasma (n=4) 90 79-100
Heparin Plasma (n=4) 85 76-95
Serum (n=4) 89 81-100
Urine (n=4) 109 100-116

Scientific Data

Human PD-L1/B7-H1 ELISA Cell Culture Supernate/Cell Lysate/Serum/Plasma Standard Curve

Human PD-L1/B7-H1 ELISA Urine Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.

Long Name:
Programmed Death Ligand 1
Entrez Gene IDs:
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names:
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. The Possible Clinical Significance of a Decreased Serum Level of Soluble PD-L1 in Discoid Lupus Erythematosus, but Not in Subacute Cutaneous Lupus Erythematosus-A Pilot Study
    Authors: Király, Z;Nagy, E;Bokor, L;Kovács, A;Marschalkó, M;Hidvégi, B;
    Journal of clinical medicine
    Species: Human
    Sample Types: Serum
  2. The screening of compounds regulating PD-L1 transcriptional activity in a cell functional high-throughput manner
    Authors: L Zhang, H Li, J Liu, G Sun, X Tang, S Xu, L Zhang, W Zhang, B Ai
    Cancer Medicine, 2023-03-25;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  3. Urine PD-L1 is a tumor tissue candidate substitute and is associated with poor survival in muscle-invasive bladder cancer patients
    Authors: J Ma, Q Zhou, W Xu, C Li, H Wang, Z Zhai, Y Zhang, W Wahafu
    International immunopharmacology, 2022-12-15;114(0):109535.
    Species: Human
    Sample Types: Urine
  4. High Pretreatment Serum PD-L1 Levels Are Associated with Muscle Invasion and Shorter Survival in Upper Tract Urothelial Carcinoma
    Authors: Á Széles, PT Kovács, A Csizmarik, M Váradi, P Riesz, T Fazekas, S Váncsa, P Hegyi, C Oláh, S Tschirdewa, C Darr, U Krafft, V Grünwald, B Hadaschik, O Horváth, P Nyirády, T Szarvas
    Biomedicines, 2022-10-13;10(10):.
    Species: Human
    Sample Types: Serum
  5. Active PD-L1 incorporation within HIV virions functionally impairs T follicular helper cells
    Authors: O Munoz, R Banga, R Schelling, FA Procopio, A Mastrangel, P Nortier, K Ohmiti, J Daraspe, M Cavassini, C Fenwick, L Perez, M Perreau
    PloS Pathogens, 2022-07-05;18(7):e1010673.
    Species: Human
    Sample Types: Plasma
  6. Circulating Levels of PD-L1, TIM-3 and MMP-7 Are Promising Biomarkers to Differentiate COVID-19 Patients That Require Invasive Mechanical Ventilation
    Authors: L Chavez-Gal, A Ruiz, K Martinez-E, H Aguilar-Du, M Torres, R Falfan-Val, G Pérez-Rubi, M Selman, I Buendia-Ro
    Biomolecules, 2022-03-14;12(3):.
    Species: Human
    Sample Types: Serum
  7. Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
    Authors: K Nakano, K Yokoyama, S Shin, K Uchida, K Tsuji, M Tanaka, K Uchimaru, T Watanabe
    Viruses, 2022-02-16;14(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Soluble PD-L1 in Serum and Urine in Urinary Bladder Cancer Patients
    Authors: A Vikerfors, S Davidsson, J Frey, T Jerlström, J Carlsson
    Cancer, 2021-11-21;13(22):.
    Species: Human
    Sample Types: Serum
  9. Protein detection in blood with single-molecule imaging
    Authors: CP Mao, SC Wang, YP Su, SH Tseng, L He, AA Wu, RBS Roden, J Xiao, CF Hung
    Science Advances, 2021-08-11;7(33):.
    Species: Human
    Sample Types: Plasma
  10. Detection of PD-L1 in the urine of patients with urothelial carcinoma of the bladder
    Authors: G Tosev, W Wahafu, P Reimold, I Damgov, C Schwab, C Aksoy, A Kaczorowsk, A Stenzinger, J Nyarangi-D, M Hohenfelln, S Duensing
    Scientific Reports, 2021-07-09;11(1):14244.
    Species: Human
    Sample Types: Urine
  11. Serum levels of soluble programmed death-ligand 1 (sPD-L1): A possible biomarker in predicting post-treatment outcomes in patients with early hepatocellular carcinoma
    Authors: T Mocan, M Ilies, I Nenu, R Craciun, A Horhat, R Susa, I Minciuna, I Rusu, LP Mocan, A Seicean, CA Iuga, NA Hajjar, M Sparchez, DC Leucuta, Z Sparchez
    International immunopharmacology, 2021-02-18;94(0):107467.
    Species: Human
    Sample Types: Serum
  12. Soluble PD-L1 improved direct ARDS by reducing monocyte-derived macrophages
    Authors: J Xu, J Wang, X Wang, R Tan, X Qi, Z Liu, H Qu, T Pan, Q Zhan, Y Zuo, W Yang, J Liu
    Cell Death Dis, 2020-10-30;11(10):934.
    Species: Human
    Sample Types: Plasma
  13. Plasma soluble PD-L1 and STAT3 predict the prognosis in diffuse large B cell lymphoma patients
    Authors: Y Fei, J Yu, Y Li, L Li, S Zhou, T Zhang, L Li, L Qiu, B Meng, Y Pan, X Ren, Z Qian, X Wang, H Zhang
    J Cancer, 2020-10-17;11(23):7001-7008.
    Species: Human
    Sample Types: Plasma
  14. Activation of JUN in fibroblasts promotes pro-fibrotic programme and modulates protective immunity
    Authors: L Cui, SY Chen, T Lerbs, JW Lee, P Domizi, S Gordon, YH Kim, G Nolan, P Betancur, G Wernig
    Nat Commun, 2020-06-03;11(1):2795.
    Species: Human
    Sample Types: Bronchoalveolar lavage fluid
  15. Soluble PD-L1 in NSCLC Patients Treated with Checkpoint Inhibitors and Its Correlation with Metabolic Parameters
    Authors: A Castello, S Rossi, L Toschi, L Mansi, E Lopci
    Cancers (Basel), 2020-05-27;12(6):.
    Species: Human
    Sample Types: Plasma
  16. Circulating Levels of PD-L1 in Mesothelioma Patients from the NIBIT-MESO-1 Study: Correlation with Survival
    Authors: C Chiarucci, S Cannito, MG Daffinà, G Amato, G Giacobini, O Cutaia, MF Lofiego, C Fazio, D Giannarell, R Danielli, AM Di Giacomo, S Coral, L Calabrò, M Maio, A Covre
    Cancers (Basel), 2020-02-05;12(2):.
    Species: Human
    Sample Types: Serum
  17. Significance of PD-L1 in the diagnosis and treatment of B-cell malignant lymphoma
    Authors: J Yang, G Hu
    Oncol Lett, 2019-01-28;17(3):3382-3386.
    Species: Human
    Sample Types: Plasma
  18. Elevated serum soluble programmed cell death ligand 1 concentration as a potential marker for poor prognosis in small cell lung cancer patients with chemotherapy
    Authors: J Jin, J Si, Y Liu, H Wang, R Ni, J Wang
    Respir. Res., 2018-10-05;19(1):197.
    Species: Human
    Sample Types: Serum
  19. Clinical significance of soluble programmed cell death ligand-1 (sPD-L1) in hepatocellular carcinoma patients treated with radiotherapy
    Authors: HJ Kim, S Park, KJ Kim, J Seong
    Radiother Oncol, 2018-01-30;0(0):.
    Species: Human
    Sample Types: Plasma

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Reviews for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

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Human PD-L1 Quantikine ELISA Kit
By Ritu Tripathi on 11/30/2018
Sample Tested: blood plasma

Human PD-L1 Quantikine ELISA Kit
By Anonymous on 10/10/2018
Sample Tested: Heparin Plasma

Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
By Leslie Priddy on 04/04/2018
Sample Tested: Serum

Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
By Anonymous on 08/02/2017
Sample Tested: A375 human melanoma cell line