Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Cystatin E/M Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin E/M
Arg29-Met149
Accession # Q15828

Specificity

Detects human Cystatin E/M in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse Cystatin E/M is observed, less than 5% cross-reactivity with recombinant human (rh) Cystatin D is observed, and less than 1% cross-reactivity with rhCystatin A, rhCystatin B, rhCystatin C, rhCystatin S, rhCystatin SA, rhCystatin SN, rhFetuin A, and rhFetuin B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Cystatin E/M Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cystatin E/M (Catalog # 1286-PI)

Reviewed Applications

Read 1 review rated 5 using BAF1286 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cystatin E/M

Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily (1, 2). It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning (1, 2). However, its protein was found only in skin and sweat glands by a third group (3). In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases (3). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age (4). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (5).

References

  1. Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
  2. Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
  3. Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
  4. Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
  5. Hong, J. et al. (2002) J. Neurochem. 81:922.

Alternate Names

CST6

Entrez Gene IDs

1474 (Human); 73720 (Mouse)

Gene Symbol

CST6

UniProt

Q15828

Additional Cystatin E/M Products

Product Documents for Human Cystatin E/M Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Human Cystatin E/M Biotinylated Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Citations for Human Cystatin E/M Biotinylated Antibody

Customer Reviews for Human Cystatin E/M Biotinylated Antibody (1)

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  • Human Cystatin E/M Biotinylated Antibody
    Name: Anonymous
    Application: Analysis of Human cancer cell lysates.
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 10/25/2020
    I used 50 μl/well, 0.2 µg/ml in PBS, pH 7.2. The result is great.
    Human Cystatin E/M Biotinylated Antibody BAF1286

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