Human Cytosolic Sulfotransferase 1B1/
SULT1B1 Antibody

Catalog #: AF5959 Datasheet / COA / SDS

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AF5959
AF5959-SP

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Detection of Human Cytosolic Sulfotransferase 1B1/SULT1B1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cytosolic Sulfotransferase 1B1/
SULT1B1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cytosolic Sulfotransferase 1B1/SULT1B1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) SULT1A1 and rhSULT1A3 is observed and less than 2% cross‑reactivity with rhSULT2A1, rhSULT4A1, rhSULT2B1, and rhSULT1E1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1B1/SULT1B1
Leu2-Ile296
Accession # O43704

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Neutralization

Measured by its ability to neutralize Recombinant Human Cytosolic Sulfotransferase 1B1/SULT1B1 (8.33 µg/mL, Catalog # 5959-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 33 μM) to 1‑Napthol (333 μM). The Neutralization Dose (ND₅₀) is typically 110 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Cytosolic Sulfotransferase 1B1/SULT1B1 by Western Blot. Western blot shows lysates of human CD4⁺T Cells and human liver tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5959) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 1B1/ SULT1B1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 1B1/SULT1B1

Cytosolic Sulfotransferases are a family of phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (1‑3). They have important functions in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. Cytosolic sulfotransferases are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchorage domains and residing only in the cytoplasm. SULT1B1 is primarily expressed in the liver, peripheral blood leukocytes, colon, spleen and small intestine and can sulfate thyroid hormones and small phenols (4). Human SULT1B1 shows 72.3% amino acid sequence identity to mouse SULT1B1.

References

Falany, C, N. (1997) FASEB J. 11:206.
Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
Allali-Hassani, A. et al. (2007) PLoS Biol. 5:e97.
Wang, J. et al. (1998) Mol. Pharmacol. 53:274.

Entrez Gene IDs

27284 (Human); 56362 (Mouse); 64305 (Rat)

Alternate Names

Cytosolic Sulfotransferase 1B1; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.1; EC 2.8.2.4; MGC13356; ST1B1; ST1B2; ST1B2sulfotransferase family cytosolic 1B member 1; Sulfotransferase 1B1; Sulfotransferase 1B2; sulfotransferase family, cytosolic, 1B, member 1; SULT1B1; SULT1B2; Thyroid hormone sulfotransferase