Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Cytosolic Sulfotransferase 1B1/SULT1B1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1B1/SULT1B1
Leu2-Ile296
Accession # O43704

Specificity

Detects human Cytosolic Sulfotransferase 1B1/SULT1B1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) SULT1A1 and rhSULT1A3 is observed and less than 2% cross‑reactivity with rhSULT2A1, rhSULT4A1, rhSULT2B1, and rhSULT1E1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Cytosolic Sulfotransferase 1B1/
SULT1B1 Antibody

Detection of Human Cytosolic Sulfotransferase 1B1/SULT1B1 antibody by Western Blot.

Detection of Human Cytosolic Sulfotransferase 1B1/SULT1B1 by Western Blot.

Western blot shows lysates of human CD4+T Cells and human liver tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5959) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 1B1/ SULT1B1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human CD4+ T Cells and human liver tissue

Neutralization

Measured by its ability to neutralize Recombinant Human Cytosolic Sulfotransferase 1B1/SULT1B1 (8.33 µg/mL, Catalog # 5959-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 33 μM) to 1‑Napthol (333 μM). The Neutralization Dose (ND50) is typically 110 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic Sulfotransferase 1B1/SULT1B1

Cytosolic Sulfotransferases are a family of phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (1‑3). They have important functions in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. Cytosolic sulfotransferases are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchorage domains and residing only in the cytoplasm. SULT1B1 is primarily expressed in the liver, peripheral blood leukocytes, colon, spleen and small intestine and can sulfate thyroid hormones and small phenols (4). Human SULT1B1 shows 72.3% amino acid sequence identity to mouse SULT1B1.

References

  1. Falany, C, N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Allali-Hassani, A. et al. (2007) PLoS Biol. 5:e97.
  4. Wang, J. et al. (1998) Mol. Pharmacol. 53:274.

Alternate Names

ST1B2, SULT1B1, SULT1B2

Entrez Gene IDs

27284 (Human); 56362 (Mouse); 64305 (Rat)

Gene Symbol

SULT1B1

UniProt

O43704

Additional Cytosolic Sulfotransferase 1B1/SULT1B1 Products

Product Documents for Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody

For research use only

Citations for Human Cytosolic Sulfotransferase 1B1/
SULT1B1 Antibody

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