Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody

Catalog # Availability Size / Price Qty
AF7458
AF7458-SP
Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cytosolic Sulfotransferase 1C2/SULT1C2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SULT1C4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 1C2/SULT1C2
Ala2-Leu296, predicted
Accession # O00338
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 by Western Blot. View Larger

Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 by Western Blot. Western blot shows lysates of Caki‑2 human clear cell carcinoma epithelial cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic Sulfotransferase 1C2/
SULT1C2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7458) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 1C2/SULT1C2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 1C2/SULT1C2

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi-resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1C2 is mainly expressed in the gastrointestinal tract (stomach, duodenum, jejunum, ileum, colon, caecum and rectum), liver and kidneys, but not in the lungs (3). In contrast, SULT1C4, a sulfotransferase that is most closely related to SULT1C2 (4), is expressed at higher levels in fetal lung and kidney and at lower levels in fetal heart. So far, SULT1C2 is found to be active only on p-nitrophenol (3). The enzymatic activity of our recombinant human SULT1C2 was determined using a phosphatase-coupled assay (5).

References
  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Hehonah, N. et al. (1999) Int J. Biochem. Cell. Biol. 31:869.
  4. Sakakibara, Y. et al. (1998) J. Biol. Chem. 273:33929.
  5. Prather, B. et al. (2012) Anal. Biochem. 423:86.
Entrez Gene IDs
6819 (Human); 316153 (Rat)
Alternate Names
Cytosolic Sulfotransferase 1C2; cytosolic, 1C, member 1; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.1; EC 2.8.2.2; humSULTC2; ST1C1; ST1C2; Sulfotransferase 1C1; sulfotransferase family, cytosolic, 1C, member 2; SULT1C#1; SULT1C1; SULT1C2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody

There are currently no reviews for this product. Be the first to review Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody and earn rewards!

Have you used Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review