Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody

AF7458 Datasheet / COA / SDS

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AF7458
AF7458-SP

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Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody in Western Blot

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Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cytosolic Sulfotransferase 1C2/SULT1C2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SULT1C4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1C2/SULT1C2
Ala2-Leu296, predicted
Accession # O00338

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 by Western Blot. Western blot shows lysates of Caki-2 human clear cell carcinoma epithelial cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic Sulfotransferase 1C2/ SULT1C2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7458) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 1C2/SULT1C2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 1C2/SULT1C2

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi-resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1C2 is mainly expressed in the gastrointestinal tract (stomach, duodenum, jejunum, ileum, colon, caecum and rectum), liver and kidneys, but not in the lungs (3). In contrast, SULT1C4, a sulfotransferase that is most closely related to SULT1C2 (4), is expressed at higher levels in fetal lung and kidney and at lower levels in fetal heart. So far, SULT1C2 is found to be active only on p-nitrophenol (3). The enzymatic activity of our recombinant human SULT1C2 was determined using a phosphatase-coupled assay (5).

References

Falany, C. N. (1997) FASEB J. 11:206.
Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
Hehonah, N. et al. (1999) Int J. Biochem. Cell. Biol. 31:869.
Sakakibara, Y. et al. (1998) J. Biol. Chem. 273:33929.
Prather, B. et al. (2012) Anal. Biochem. 423:86.

Entrez Gene IDs

6819 (Human); 316153 (Rat)

Alternate Names

Cytosolic Sulfotransferase 1C2; cytosolic, 1C, member 1; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.1; EC 2.8.2.2; humSULTC2; ST1C1; ST1C2; Sulfotransferase 1C1; sulfotransferase family, cytosolic, 1C, member 2; SULT1C#1; SULT1C1; SULT1C2