Human DAP12 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5240G-100UG
Detection of DAP12 in Human PBMCs by Flow Cytometry.
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Human DAP12 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DAP12 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 406288
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human DAP12 synthetic peptide
QGQRSDVYSDLNTQRPYYK
Accession # O43914
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of DAP12 in Human PBMCs by Flow Cytometry. View Larger

Detection of DAP12 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DAP12 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5240G) or (B) Mouse IgG1 Alexa Fluor® 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DAP12

DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.

Long Name
DNAX-activation Protein 12
Entrez Gene IDs
7305 (Human); 22177 (Mouse); 361537 (Rat)
Alternate Names
DAP12; DAP12PLOSL; DNAX-activation protein 12; KARAP; KARAPPLO-SL; KAR-associated protein; killer activating receptor associated protein; Killer-activating receptor-associated protein; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein; TYROBP

Product Datasheets

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