Human DIDO1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human DIDO1 by Western Blot.
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Product Details
Supplemental Products

Human DIDO1 Antibody Summary

Species Reactivity
Detects human DIDO1 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human DIDO1
Accession # Q9BTC0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DIDO1 antibody by Western Blot. View Larger

Detection of Human DIDO1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human DIDO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DIDO1 at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DIDO1

DIDO1 (Death-inducer obliterator 1; also DIO-1, C20orf158 and DATF-1) is a 245 kDa (predicted) intracellular protein that belongs the DIO family of proteins. It is ubiquitous in expression, and apparently serves multiple functions, depending on its splice variant. The standard form is a nuclear protein that maintains the integrity of mitotic checkpoint protein BubR1. An absence of DIDO1 results in centrosome amplification and unequal chromatin segregation. The best known short form (an 83 kDa isoform in mouse), by contrast, induces apoptosis by promoting transcription of caspase 9. Human standard form DIDO1 is 2240 amino acid (aa) in length. It contains two NLSs (aa 165-193), one PHD-type Zn finger region (aa 270-320), a TFIIS domain (aa 670-790), an SPOC protein-interaction domain (aa 1057-1163), two Pro-rich segments (aa 1257-1286 and 1725-2034) and a C-terminal Arg-rich region (aa 2108-2214). There are least 23 utilized phosphoryation sites. Multiple splice forms exist. Variants are generated by short aa substitutions for aa 531-2240, 566-2240 and 1182-2240, possibly coupled to a 36 aa insertion after Pro387. Over aa 1‑117, human DIDO1 shares 80% aa identity with mouse DIDO1.

Long Name
Death Inducer Obliterator 1
Entrez Gene IDs
11083 (Human); 23856 (Mouse); 362286 (Rat)
Alternate Names
BYE1; C20orf158; DATF1; DATF-1; DATF1DIDO3; death associated transcription factor 1; death inducer-obliterator 1; Death-associated transcription factor 1; death-inducer obliterator 1; DIDO1; DIO1; DIO1DIDO2; DIO-1hDido1; dJ885L7.8; DKFZp434P1115; FLJ11265; KIAA0333chromosome 20 open reading frame 158; MGC16140

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