Human DIDO1 Antibody

DIDO1 (Death-inducer obliterator 1; also DIO-1, C20orf158 and DATF-1) is a 245 kDa (predicted) intracellular protein that belongs the DIO family of proteins. It is ubiquitous in expression, and apparently serves multiple functions, depending on its splice variant. The standard form is a nuclear protein that maintains the integrity of mitotic checkpoint protein BubR1. An absence of DIDO1 results in centrosome amplification and unequal chromatin segregation. The best known short form (an 83 kDa isoform in mouse), by contrast, induces apoptosis by promoting transcription of caspase 9. Human standard form DIDO1 is 2240 amino acid (aa) in length. It contains two NLSs (aa 165-193), one PHD-type Zn finger region (aa 270-320), a TFIIS domain (aa 670-790), an SPOC protein-interaction domain (aa 1057-1163), two Pro-rich segments (aa 1257-1286 and 1725-2034) and a C-terminal Arg-rich region (aa 2108-2214). There are least 23 utilized phosphoryation sites. Multiple splice forms exist. Variants are generated by short aa substitutions for aa 531-2240, 566-2240 and 1182-2240, possibly coupled to a 36 aa insertion after Pro387. Over aa 1‑117, human DIDO1 shares 80% aa identity with mouse DIDO1.