Human Dkk-1 Biotinylated Antibody

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Product Details
Citations (8)
Supplemental Products
Reviews (1)

Human Dkk-1 Biotinylated Antibody Summary

Species Reactivity
Detects human Dkk-1 in ELISAs and Western blots. In sandwich immunoassays, approximately 85% cross‑reactivity with rmDkk-1 is observed and less than 0.2% cross‑reactivity with rhDkk-4 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Accession # O94907
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Dkk‑1 (Catalog # 5439-DK)

Human Dkk-1 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Dkk‑1 Antibody (Catalog # MAB10962)

Standard: Recombinant Human Dkk-1 Protein (Catalog # 5439-DK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is a member of the Dkk protein family that includes Dkk-1, -2, -3, and -4 (1). All four members are secreted proteins that are synthesized as precursor proteins with an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region. Dkk proteins have potential furin type proteolytic cleavage sites, and short forms of Dkk-2 and Dkk-4 containing only the second cysteine-rich domain can be generated by proteolytic processing (1). Dkk proteins have distinct patterns of expression in adult and embryonic tissues, suggesting that they may play diverse roles in these tissues. The Dkk proteins have distinct effects on Wnt signaling. Dkk-1 and Dkk-4 are Wnt antagonists. Dkk-3 has not been demonstrated to affect Wnt signaling, and Dkk-2 acts as an agonist or antagonist, depending on the cellular context. Wnt signaling regulates many important developmental processes including neural crest differentiation, brain development, kidney morphogenesis, and sex determination. In addition, Wnt signaling has also been implicated in tumor formation. Canonical Wnt signaling via the beta-catenin pathway is transduced by a receptor complex composed of the Frizzled proteins (Fz) and low-density lipoprotein (LDL) receptor-related proteins (LRP5/6) (2, 3). Unlike many soluble Wnt antagonists that function by binding extracellular Wnt ligands to prevent interaction of Wnt with the Fz-LRP5/6 receptor complex, Dkk-1 and Dkk-4 antagonize Wnt/ beta -catenin signaling by direct high-affinity binding to the Wnt coreceptor LRP5/6 and inhibiting interaction of LRP5/6 with the Wnt-Frizzled complex (4). Dkk-1 and Dkk-4 also bind the transmembrane proteins Kremen1 (Krm1) and Krm2 with high-affinity (5). Krm2 has been shown to form a ternary complex with Dkk-1 or -4 and LRP5/6 to trigger internalization of the complex and removal LRP6 from the cell surface. Thus, Dkk-1/4 and Kremens function synergistically to antagonize LRP5/6-mediated Wnt activity. Dkk-2 also binds to LRP5/6 and the Kremens, but Dkk-2 acts as antagonist of the Wnt signaling pathway only in the presence of Krm2 (5, 6). Dkk 2 binding to LRP5/6 in the absence of Krm2 activates rather than inhibits Wnt signaling (6).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.
  2. Zorn, A.M (2001) Current Biology R592.
  3. Mao, J. et al. (2001) Mol. Cell 7:801.
  4. Nusse, R. et al. (2001) Nature 411:255.
  5. Mao, J. et al. (2002) Nature 417:664.
  6. Mao, B. and C. Niehrs (2003) Gene 302:179.
Long Name
Entrez Gene IDs
22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)
Alternate Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like

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Citations for Human Dkk-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. CSN5/JAB1 suppresses the WNT inhibitor DKK1 in colorectal cancer cells
    Authors: S Jumpertz, T Hennes, Y Asare, AK Schütz, J Bernhagen
    Cell. Signal, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Thiazolidinediones increase the wingless-type MMTV integration site family (WNT) inhibitor Dickkopf-1 in adipocytes: a link with osteogenesis.
    Authors: Gustafson B, Eliasson B, Smith U
    Diabetologia, 2010;53(3):536-40.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  3. Decreased osteogenesis, increased cell senescence and elevated Dickkopf-1 secretion in human fracture non union stromal cells.
    Authors: Bajada S, Marshall MJ, Wright KT, Richardson JB, Johnson WE
    Bone, 2009;45(4):726-35.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Serum concentrations of DKK-1 decrease in patients with multiple myeloma responding to anti-myeloma treatment.
    Authors: Heider U, Kaiser M, Mieth M, Lamottke B, Rademacher J, Jakob C, Braendle E, Stover D, Sezer O
    Eur. J. Haematol., 2009;82(1):31-8.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  5. Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma.
    Authors: Qiang YW, Chen Y, Stephens O, Brown N, Chen B, Epstein J, Barlogie B, Shaughnessy JD
    Blood, 2008;112(1):196-207.
    Species: Human
    Sample Types: Bone Marrow Fluid
    Applications: ELISA Development
  6. Wnt signaling antagonists are potential prognostic biomarkers for the progression of radiographic hip osteoarthritis in elderly Caucasian women.
    Authors: Lane NE, Nevitt MC, Lui LY, de Leon P, Corr M
    Arthritis Rheum., 2007;56(10):3319-25.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  7. Differential gene expression in cultured osteoblasts and bone marrow stromal cells from patients with Paget's disease of bone.
    Authors: Naot D, Bava U, Matthews B, Callon KE, Gamble GD, Black M, Song S, Pitto RP, Cundy T, Cornish J, Reid IR
    J. Bone Miner. Res., 2007;22(2):298-309.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  8. Dickkopf-1 is a master regulator of joint remodeling.
    Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
    Nat. Med., 2007;13(2):156-63.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development


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Human Dkk-1 Biotinylated Antibody
By Anonymous on 12/17/2020
Application: ELISA Sample Tested: Recombinant protein Species: Human