Human DLEC/CLEC4C/BDCA-2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1376

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Human DLEC/CLEC4C/BDCA-2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DLEC/CLEC4C/BDCA‑2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DLEC/CLEC4C/BDCA‑2
Phe46-Ile213
Accession # Q8WTT0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human DLEC/CLEC4C/BDCA2 Fc Chimera (Catalog # 1376-DL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile Tris-buffered saline, pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% bovine serum albumin.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLEC/CLEC4C/BDCA-2

Dendritic cell lectin (DLEC), also known as BDCA-2, CD303, HECL, and CLEC4C/CLECSF11/CLECSF7, is a 38 kDa type II transmembrane protein in the C-type lectin family (1). Mature human DLEC consists of a 21 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain (ECD) that contains a juxtamembrane neck region and one carbohydrate recognition domain (CRD) (2, 3). Alternate splicing may generate multiple isoforms that lack the transmembrane segment and/or portions of the cytoplasmic, neck, and CRD regions (2-4). An ortholog of human DLEC has not been described in mouse or rat. DLEC expression is restricted to plasmacytoid dendritic cells (pDC) and is downregulated during their maturation (2, 3, 5). pDC play a role in the innate immune response by producing IFN-alpha / beta following exposure to TLR7 and TLR9 agonists such as microbial CpG DNA (3, 5-8). Antibody ligation of DLEC on pDC attenuates the CpG-stimulated production of interferons as well as a Th1 biased response (3, 5-9). DLEC interactions with HIV-1 gp120 and hepatitis B virus soluble antigen may therefore limit the pDC antiviral response (10, 11). Similar to other C-type lectins, DLEC can mediate antigen uptake for MHC loading and presentation to T cells (3, 12). Crosslinking of DLEC on CpG-stimulated pDC inhibits pDC maturation and induces tyrosine phosphorylation on multiple proteins involved in B cell receptor signaling and endocytosis (5, 7, 8). These functions require the association of DLEC with the ITAM-containing Fc epsilon RI gamma chain (7, 8).

References
  1. Kanazawa, N. et al. (2004) Immunobiology 209:179.
  2. Arce, I. et al. (2001) Eur. J. Immunol. 31:2733.
  3. Dzionek, A. et al. (2001) J. Exp. Med. 194:1823.
  4. Fernandes, M.J. et al. (2000) Genomics 69:263.
  5. Wu, P. et al. (2008) Clin. Immunol. 129:40.
  6. Fanning, S.L. et al. (2006) J. Immunol. 177:5829.
  7. Rock, J. et al. (2007) Eur. J. Immunol. 37:3564.
  8. Cao, W. et al. (2007) PloS Biol. 5:e248.
  9. Riboldi, E. et al. (2009) Immunobiology 214:868.
  10. Martinelli, E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:3396.
  11. Xu, Y. et al. (2009) Mol. Immunol. 46:2640.
  12. Jaehn, P.S. et al. (2008) Eur. J. Immunol. 38:1822.
Long Name
Dendritic Cell Lectin
Entrez Gene IDs
170482 (Human)
Alternate Names
BDCA2; BDCA-2; BDCA2MGC125791; blood dendritic cell antigen 2 protein; Blood dendritic cell antigen 2; CD303 antigen; CD303; CLEC4C; CLECSF11; CLECSF11PRO34150; CLECSF7; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 11; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 7; C-type lectin domain family 4 member C; C-type lectin superfamily member 7; dendritic cell lectin b; Dendritic lectin; DLEC; DLECMGC125792; HECL; HECLMGC125793; MGC125789; PRO34150

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