Human DLEC/CLEC4C/BDCA-2 Antibody Summary
Accession # Q8WTT0
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of DLEC/CLEC4C/BDCA‑2 in Human Whole Blood by Flow Cytometry. Human whole blood were stained with Goat Anti- Human DLEC/CLEC4C/BDCA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1376) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and Human IL-3 Ra Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB301P).Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).
Detection of DLEC/CLEC4C/BDCA‑2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL-3 Ra/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Goat Anti-Human DLEC/CLEC4C/BDCA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1376) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
DLEC/CLEC4C/BDCA-2 in Human Lymph Node. DLEC/CLEC4C/BDCA-2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human DLEC/CLEC4C/BDCA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1376) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
DLEC/CLEC4C/BDCA‑2 in Human Tonsil. DLEC/CLEC4C/BDCA-2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human DLEC/CLEC4C/BDCA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1376) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Dendritic cell lectin (DLEC), also known as BDCA-2, CD303, HECL, and CLEC4C/CLECSF11/CLECSF7, is a 38 kDa type II transmembrane protein in the C-type lectin family (1). Mature human DLEC consists of a 21 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain (ECD) that contains a juxtamembrane neck region and one carbohydrate recognition domain (CRD) (2, 3). Alternate splicing may generate multiple isoforms that lack the transmembrane segment and/or portions of the cytoplasmic, neck, and CRD regions (2-4). An ortholog of human DLEC has not been described in mouse or rat. DLEC expression is restricted to plasmacytoid dendritic cells (pDC) and is downregulated during their maturation (2, 3, 5). pDC play a role in the innate immune response by producing IFN-alpha / beta following exposure to TLR7 and TLR9 agonists such as microbial CpG DNA (3, 5-8). Antibody ligation of DLEC on pDC attenuates the CpG-stimulated production of interferons as well as a Th1 biased response (3, 5-9). DLEC interactions with HIV-1 gp120 and hepatitis B virus soluble antigen may therefore limit the pDC antiviral response (10, 11). Similar to other C-type lectins, DLEC can mediate antigen uptake for MHC loading and presentation to T cells (3, 12). Crosslinking of DLEC on CpG-stimulated pDC inhibits pDC maturation and induces tyrosine phosphorylation on multiple proteins involved in B cell receptor signaling and endocytosis (5, 7, 8). These functions require the association of DLEC with the ITAM-containing Fc epsilon RI gamma chain (7, 8).
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- Riboldi, E. et al. (2009) Immunobiology 214:868.
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Citations for Human DLEC/CLEC4C/BDCA-2 Antibody
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Citations: Showing 1 - 3
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Clinical Significance of Tumor-Infiltrating Conventional and Plasmacytoid Dendritic Cells in Pancreatic Ductal Adenocarcinoma
Authors: I Plesca, I Benešová, C Beer, U Sommer, L Müller, R Wehner, M Heiduk, D Aust, G Baretton, MP Bachmann, A Feldmann, J Weitz, L Seifert, AM Seifert, M Schmitz
Sample Types: Whole Cells
Tissue-specific transcriptional profiling of plasmacytoid dendritic cells reveals a hyperactivated state in chronic SIV infection
Authors: MY Lee, AA Upadhyay, H Walum, CN Chan, RA Dawoud, C Grech, JL Harper, KA Karunakara, SA Nelson, EA Mahar, KL Goss, DG Carnathan, B Cervasi, K Gill, GK Tharp, ER Wonderlich, V Velu, SM Barratt-Bo, M Paiardini, G Silvestri, JD Estes, SE Bosinger
PloS Pathogens, 2021;17(6):e1009674.
Sample Types: Whole Tissue
In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa.
Authors: Hirbod T, Kaldensjo T, Broliden K
PLoS ONE, 2011;6(9):e25551.
Sample Types: Whole Tissue
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