Human DLL4 Antibody

Catalog # Availability Size / Price Qty
MAB1506
MAB1506-SP
Detection of DLL4 in HUVEC Human Cells by Flow Cytometry.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (4)

Human DLL4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DLL4 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 447506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DLL4
Ser27-Pro524
Accession # Q9NR61
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DLL4 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of DLL4 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

Immunocytochemistry DLL4 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

DLL4 in HUVEC Human Cells. DLL4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL4

Delta-like ligand 4 (DLL4) is a transmembrane protein that contains one DSL domain and eight tandem EGF-like repeats. DLL4 is expressed on arterial endothelial cells where it signals through Notch 1 and Notch 4. DLL4 expression is induced by VEGF and regulates the proliferation of endothelial tip cells during vascular sprouting. DLL4 blockade as well as overexpression can suppress tumor growth by promoting dysregulated angiogenesis. Within the extracellular domain, human DLL4 shares 85% amino acid sequence identity with mouse and rat DLL4.

Long Name
Delta-like 4
Entrez Gene IDs
54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names
Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4

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Citations for Human DLL4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Concurrent Treatment with Anti-DLL4 Enhances Antitumor and Proapoptotic Efficacy of a ?-Secretase Inhibitor in Gastric Cancer
    Authors: M Kang, Y Zhang, X Jin, G Chen, Y Huang, D Wu, G Li, J Shan, P Huang, J Chen
    Transl Oncol, 2018;11(3):599-608.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. NOTCH Activation at the Hematovascular Mesoderm Stage Facilitates Efficient Generation of T Cells with High Proliferation Potential from Human Pluripotent Stem Cells
    Authors: A Kumar, JH Lee, K Suknuntha, SS D'Souza, AS Thakur, II Slukvin
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human DLL4 Antibody

Average Rating: 4.3 (Based on 4 Reviews)

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Human DLL4 Antibody
By Anonymous on 12/02/2020
Application: ICC/IF Sample Tested: E10.5 mouse embryo fixed in 4% PFA Species: Mouse

Staining was done on E10.5 mouse sections (pharyngeal mesoderm).
Note the arteries being positive and adjacent veins staining negative for Dll4.


By Jignesh Ganeshbhai Patel on 09/10/2018
Application: WB Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

By Jignesh Patel on 09/10/2018
Application: ICC/IF Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

By Jignesh Patel on 08/21/2018
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human