Human DPP6 Antibody

Catalog # Availability Size / Price Qty
MAB2360
MAB2360-SP
Detection of DPP6 in Human SHSY5Y Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Human DPP6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DPP6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPPIV is observed.
Source
Monoclonal Mouse IgG2B Clone # 274308
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DPP6
Thr56-Asp803 (Glu458Val)
Accession # AAA35761
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
SHSY5Y human neuroblastoma cell line or human differentiated neuron progenitor cells
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human DPP6, see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of DPP6 in Human SHSY5Y Cell Line by Flow Cytometry. Human SHSY5Y neuroblastoma cell line was stained with Mouse Anti-Human DPP6 Monoclonal Antibody (Catalog # MAB2360, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPP6

DPP6, also known as DPPX and DPPIV/CD26 related protein, is a critical component of neuronal A-type K+ channels but lacks protease activity.

Long Name
Dipeptidyl-peptidase 6
Entrez Gene IDs
1804 (Human)
Alternate Names
dipeptidyl aminopeptidase IV-related protein; dipeptidyl aminopeptidase-like protein 6; Dipeptidyl aminopeptidase-related protein; Dipeptidyl peptidase 6; Dipeptidyl peptidase IV-like protein; dipeptidyl peptidase IV-related protein; Dipeptidyl peptidase VI; dipeptidylpeptidase 6; dipeptidyl-peptidase 6; dipeptidylpeptidase VI; DPP VI; DPP6; DPPX; DPPXFLJ55680; MGC46605; VF2

Product Datasheets

Citation for Human DPP6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A nanobody-based tracer targeting DPP6 for non-invasive imaging of human pancreatic endocrine cells
    Authors: A Balhuizen, S Massa, I Mathijs, JV Turatsinze, J De Vos, S Demine, C Xavier, O Villate, I Millard, D Egrise, C Capito, R Scharfmann, P In't Veld, P Marchetti, S Muylderman, S Goldman, T Lahoutte, L Bouwens, DL Eizirik, N Devoogdt
    Sci Rep, 2017;7(1):15130.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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