Human DPP6 Antibody

Detection of DPP6 in Human SHSY5Y Cell Line by Flow Cytometry. Human SHSY5Y neuroblastoma cell line was stained with Mouse Anti-Human DPP6 Monoclonal Antibody (Catalog # MAB2360, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane Proteins protocol.

Detection of Human DPP6 by Immunohistochemistry Localization of DPP6 expression in human pancreas. (A–E) A representative human pancreas stained for DPP6 (A, red), insulin (B, white), somatostatin (C, green); overlay of DPP6 (red), insulin (white) and somatostatin (green) (D); overlay of DPP6 (red) and somatostatin (green) (E); the data indicate co-staining of insulin and DPP6, but not somatostatin and DPP6; (F–J) A representative human pancreas stained for glucagon (F, green), DPP6 (G, red), insulin (H, white); DPP6 (red) and glucagon (green) overlay (I); overlay of DPP6 (red), insulin (white) and glucagon (green) (J), the data indicate co-staining of both insulin and glucagon with DPP6; (K) Morphometric quantification of DPP6 area in pancreata from T1D patients as compared to control, non-diabetic individuals (n = 3). (L–P) A representative human pancreas from a subject with long-term type 1 diabetes (16 years of disease) stained for glucagon (L, green), DPP6 (M, red), insulin (N, white); Hoechst (O, blue); (P) overlay of DPP6 (green), glucagon (red), insulin (white) and Hoechst (blue), indicating that in the absence of insulin positive cells, the remaining glucagon positive cells co-stain for DPP6. In total, 3 pancreata from normoglycemic individuals and 3 from type 1 diabetes subjects were analysed. White scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunohistochemistry Localization of DPP6 expression in human pancreas. (A–E) A representative human pancreas stained for DPP6 (A, red), insulin (B, white), somatostatin (C, green); overlay of DPP6 (red), insulin (white) and somatostatin (green) (D); overlay of DPP6 (red) and somatostatin (green) (E); the data indicate co-staining of insulin and DPP6, but not somatostatin and DPP6; (F–J) A representative human pancreas stained for glucagon (F, green), DPP6 (G, red), insulin (H, white); DPP6 (red) and glucagon (green) overlay (I); overlay of DPP6 (red), insulin (white) and glucagon (green) (J), the data indicate co-staining of both insulin and glucagon with DPP6; (K) Morphometric quantification of DPP6 area in pancreata from T1D patients as compared to control, non-diabetic individuals (n = 3). (L–P) A representative human pancreas from a subject with long-term type 1 diabetes (16 years of disease) stained for glucagon (L, green), DPP6 (M, red), insulin (N, white); Hoechst (O, blue); (P) overlay of DPP6 (green), glucagon (red), insulin (white) and Hoechst (blue), indicating that in the absence of insulin positive cells, the remaining glucagon positive cells co-stain for DPP6. In total, 3 pancreata from normoglycemic individuals and 3 from type 1 diabetes subjects were analysed. White scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunohistochemistry Localization of DPP6 expression in human pancreas. (A–E) A representative human pancreas stained for DPP6 (A, red), insulin (B, white), somatostatin (C, green); overlay of DPP6 (red), insulin (white) and somatostatin (green) (D); overlay of DPP6 (red) and somatostatin (green) (E); the data indicate co-staining of insulin and DPP6, but not somatostatin and DPP6; (F–J) A representative human pancreas stained for glucagon (F, green), DPP6 (G, red), insulin (H, white); DPP6 (red) and glucagon (green) overlay (I); overlay of DPP6 (red), insulin (white) and glucagon (green) (J), the data indicate co-staining of both insulin and glucagon with DPP6; (K) Morphometric quantification of DPP6 area in pancreata from T1D patients as compared to control, non-diabetic individuals (n = 3). (L–P) A representative human pancreas from a subject with long-term type 1 diabetes (16 years of disease) stained for glucagon (L, green), DPP6 (M, red), insulin (N, white); Hoechst (O, blue); (P) overlay of DPP6 (green), glucagon (red), insulin (white) and Hoechst (blue), indicating that in the absence of insulin positive cells, the remaining glucagon positive cells co-stain for DPP6. In total, 3 pancreata from normoglycemic individuals and 3 from type 1 diabetes subjects were analysed. White scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunohistochemistry Localization of DPP6 expression in human pancreas. (A–E) A representative human pancreas stained for DPP6 (A, red), insulin (B, white), somatostatin (C, green); overlay of DPP6 (red), insulin (white) and somatostatin (green) (D); overlay of DPP6 (red) and somatostatin (green) (E); the data indicate co-staining of insulin and DPP6, but not somatostatin and DPP6; (F–J) A representative human pancreas stained for glucagon (F, green), DPP6 (G, red), insulin (H, white); DPP6 (red) and glucagon (green) overlay (I); overlay of DPP6 (red), insulin (white) and glucagon (green) (J), the data indicate co-staining of both insulin and glucagon with DPP6; (K) Morphometric quantification of DPP6 area in pancreata from T1D patients as compared to control, non-diabetic individuals (n = 3). (L–P) A representative human pancreas from a subject with long-term type 1 diabetes (16 years of disease) stained for glucagon (L, green), DPP6 (M, red), insulin (N, white); Hoechst (O, blue); (P) overlay of DPP6 (green), glucagon (red), insulin (white) and Hoechst (blue), indicating that in the absence of insulin positive cells, the remaining glucagon positive cells co-stain for DPP6. In total, 3 pancreata from normoglycemic individuals and 3 from type 1 diabetes subjects were analysed. White scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunohistochemistry Localization of DPP6 expression in human pancreas. (A–E) A representative human pancreas stained for DPP6 (A, red), insulin (B, white), somatostatin (C, green); overlay of DPP6 (red), insulin (white) and somatostatin (green) (D); overlay of DPP6 (red) and somatostatin (green) (E); the data indicate co-staining of insulin and DPP6, but not somatostatin and DPP6; (F–J) A representative human pancreas stained for glucagon (F, green), DPP6 (G, red), insulin (H, white); DPP6 (red) and glucagon (green) overlay (I); overlay of DPP6 (red), insulin (white) and glucagon (green) (J), the data indicate co-staining of both insulin and glucagon with DPP6; (K) Morphometric quantification of DPP6 area in pancreata from T1D patients as compared to control, non-diabetic individuals (n = 3). (L–P) A representative human pancreas from a subject with long-term type 1 diabetes (16 years of disease) stained for glucagon (L, green), DPP6 (M, red), insulin (N, white); Hoechst (O, blue); (P) overlay of DPP6 (green), glucagon (red), insulin (white) and Hoechst (blue), indicating that in the absence of insulin positive cells, the remaining glucagon positive cells co-stain for DPP6. In total, 3 pancreata from normoglycemic individuals and 3 from type 1 diabetes subjects were analysed. White scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunocytochemistry/ Immunofluorescence Expression of DPP6 in human islets and EndoC-beta H1 cells and other tissues evaluated by qPCR and histology. (A) Quantitative RT-PCR (qPCR) of DPP6 mRNA expression (detecting a shared sequence among all DPP6 splice variants) in EndoC-beta H1 cells (n = 5) and human pancreatic islets (n = 4) that were exposed or not to cytokines (IL-1 beta  + IFN-gamma ) for 48 h, as compared to pancreatic exocrine tissue (n = 6), two exocrine cell lines (Capan-2 (n = 3) and PANC (n = 3)), and 14 other non-pathological human tissues (n = 1). (B) Immunoblot of EndoC-beta H1 cells under control conditions or following a 48h exposure to cytokines (IL-1 beta and IFN-gamma ), with alpha-tubulin as a reference protein. A representative figure is shown at the top and densitometric analysis at the bottom (n = 5), this figure displays a cropped blot, the full-length version is included in supplementary figure 8; (C–F) Immunocytochemistry of EndoC-beta H1 cells. (C) An overlay with cells stained with an anti-DPP6 monoclonal antibody (mAb, red), co-stained for insulin (green) and Hoechst in blue. The separate channels are displayed in (D) insulin (green) and (E) DPP6 (red) (n = 3). The mostly surface localization of DPP6 (red) can be observed in (F) (n = 4), with blue signals indicating Hoechst staining. The negative staining control of EndoC-beta H1 cells (without the DPP6 antibody) is placed in the top right corner of panel F. White scale bar represents 1 µm. RT-qPCR and the western blot data are presented as means ± SEM. Paired and unpaired two-way ANOVA (indicated with * and $, respectively), and unpaired one-way ANOVA (indicated with #) with Šídák correction for multiple comparisons; *, $ and # p ≤ 0.05 as indicated by bars. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunohistochemistry Localization of DPP6 expression in human pancreas. (A–E) A representative human pancreas stained for DPP6 (A, red), insulin (B, white), somatostatin (C, green); overlay of DPP6 (red), insulin (white) and somatostatin (green) (D); overlay of DPP6 (red) and somatostatin (green) (E); the data indicate co-staining of insulin and DPP6, but not somatostatin and DPP6; (F–J) A representative human pancreas stained for glucagon (F, green), DPP6 (G, red), insulin (H, white); DPP6 (red) and glucagon (green) overlay (I); overlay of DPP6 (red), insulin (white) and glucagon (green) (J), the data indicate co-staining of both insulin and glucagon with DPP6; (K) Morphometric quantification of DPP6 area in pancreata from T1D patients as compared to control, non-diabetic individuals (n = 3). (L–P) A representative human pancreas from a subject with long-term type 1 diabetes (16 years of disease) stained for glucagon (L, green), DPP6 (M, red), insulin (N, white); Hoechst (O, blue); (P) overlay of DPP6 (green), glucagon (red), insulin (white) and Hoechst (blue), indicating that in the absence of insulin positive cells, the remaining glucagon positive cells co-stain for DPP6. In total, 3 pancreata from normoglycemic individuals and 3 from type 1 diabetes subjects were analysed. White scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunocytochemistry/ Immunofluorescence Expression of DPP6 in human islets and EndoC-beta H1 cells and other tissues evaluated by qPCR and histology. (A) Quantitative RT-PCR (qPCR) of DPP6 mRNA expression (detecting a shared sequence among all DPP6 splice variants) in EndoC-beta H1 cells (n = 5) and human pancreatic islets (n = 4) that were exposed or not to cytokines (IL-1 beta  + IFN-gamma ) for 48 h, as compared to pancreatic exocrine tissue (n = 6), two exocrine cell lines (Capan-2 (n = 3) and PANC (n = 3)), and 14 other non-pathological human tissues (n = 1). (B) Immunoblot of EndoC-beta H1 cells under control conditions or following a 48h exposure to cytokines (IL-1 beta and IFN-gamma ), with alpha-tubulin as a reference protein. A representative figure is shown at the top and densitometric analysis at the bottom (n = 5), this figure displays a cropped blot, the full-length version is included in supplementary figure 8; (C–F) Immunocytochemistry of EndoC-beta H1 cells. (C) An overlay with cells stained with an anti-DPP6 monoclonal antibody (mAb, red), co-stained for insulin (green) and Hoechst in blue. The separate channels are displayed in (D) insulin (green) and (E) DPP6 (red) (n = 3). The mostly surface localization of DPP6 (red) can be observed in (F) (n = 4), with blue signals indicating Hoechst staining. The negative staining control of EndoC-beta H1 cells (without the DPP6 antibody) is placed in the top right corner of panel F. White scale bar represents 1 µm. RT-qPCR and the western blot data are presented as means ± SEM. Paired and unpaired two-way ANOVA (indicated with * and $, respectively), and unpaired one-way ANOVA (indicated with #) with Šídák correction for multiple comparisons; *, $ and # p ≤ 0.05 as indicated by bars. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunocytochemistry/ Immunofluorescence Expression of DPP6 in human islets and EndoC-beta H1 cells and other tissues evaluated by qPCR and histology. (A) Quantitative RT-PCR (qPCR) of DPP6 mRNA expression (detecting a shared sequence among all DPP6 splice variants) in EndoC-beta H1 cells (n = 5) and human pancreatic islets (n = 4) that were exposed or not to cytokines (IL-1 beta  + IFN-gamma ) for 48 h, as compared to pancreatic exocrine tissue (n = 6), two exocrine cell lines (Capan-2 (n = 3) and PANC (n = 3)), and 14 other non-pathological human tissues (n = 1). (B) Immunoblot of EndoC-beta H1 cells under control conditions or following a 48h exposure to cytokines (IL-1 beta and IFN-gamma ), with alpha-tubulin as a reference protein. A representative figure is shown at the top and densitometric analysis at the bottom (n = 5), this figure displays a cropped blot, the full-length version is included in supplementary figure 8; (C–F) Immunocytochemistry of EndoC-beta H1 cells. (C) An overlay with cells stained with an anti-DPP6 monoclonal antibody (mAb, red), co-stained for insulin (green) and Hoechst in blue. The separate channels are displayed in (D) insulin (green) and (E) DPP6 (red) (n = 3). The mostly surface localization of DPP6 (red) can be observed in (F) (n = 4), with blue signals indicating Hoechst staining. The negative staining control of EndoC-beta H1 cells (without the DPP6 antibody) is placed in the top right corner of panel F. White scale bar represents 1 µm. RT-qPCR and the western blot data are presented as means ± SEM. Paired and unpaired two-way ANOVA (indicated with * and $, respectively), and unpaired one-way ANOVA (indicated with #) with Šídák correction for multiple comparisons; *, $ and # p ≤ 0.05 as indicated by bars. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunohistochemistry Localization of DPP6 expression in human pancreas. (A–E) A representative human pancreas stained for DPP6 (A, red), insulin (B, white), somatostatin (C, green); overlay of DPP6 (red), insulin (white) and somatostatin (green) (D); overlay of DPP6 (red) and somatostatin (green) (E); the data indicate co-staining of insulin and DPP6, but not somatostatin and DPP6; (F–J) A representative human pancreas stained for glucagon (F, green), DPP6 (G, red), insulin (H, white); DPP6 (red) and glucagon (green) overlay (I); overlay of DPP6 (red), insulin (white) and glucagon (green) (J), the data indicate co-staining of both insulin and glucagon with DPP6; (K) Morphometric quantification of DPP6 area in pancreata from T1D patients as compared to control, non-diabetic individuals (n = 3). (L–P) A representative human pancreas from a subject with long-term type 1 diabetes (16 years of disease) stained for glucagon (L, green), DPP6 (M, red), insulin (N, white); Hoechst (O, blue); (P) overlay of DPP6 (green), glucagon (red), insulin (white) and Hoechst (blue), indicating that in the absence of insulin positive cells, the remaining glucagon positive cells co-stain for DPP6. In total, 3 pancreata from normoglycemic individuals and 3 from type 1 diabetes subjects were analysed. White scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human DPP6 by Immunohistochemistry Localization of DPP6 expression in human pancreas. (A–E) A representative human pancreas stained for DPP6 (A, red), insulin (B, white), somatostatin (C, green); overlay of DPP6 (red), insulin (white) and somatostatin (green) (D); overlay of DPP6 (red) and somatostatin (green) (E); the data indicate co-staining of insulin and DPP6, but not somatostatin and DPP6; (F–J) A representative human pancreas stained for glucagon (F, green), DPP6 (G, red), insulin (H, white); DPP6 (red) and glucagon (green) overlay (I); overlay of DPP6 (red), insulin (white) and glucagon (green) (J), the data indicate co-staining of both insulin and glucagon with DPP6; (K) Morphometric quantification of DPP6 area in pancreata from T1D patients as compared to control, non-diabetic individuals (n = 3). (L–P) A representative human pancreas from a subject with long-term type 1 diabetes (16 years of disease) stained for glucagon (L, green), DPP6 (M, red), insulin (N, white); Hoechst (O, blue); (P) overlay of DPP6 (green), glucagon (red), insulin (white) and Hoechst (blue), indicating that in the absence of insulin positive cells, the remaining glucagon positive cells co-stain for DPP6. In total, 3 pancreata from normoglycemic individuals and 3 from type 1 diabetes subjects were analysed. White scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29123178), licensed under a CC-BY license. Not internally tested by R&D Systems.