hh Human DTX1/DTX4 Antibody MAB7157: R&D Systems

Human DTX1/DTX4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human DTX1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human DTX4 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 730612
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human DTX1/DTX4
    Met1-Phe147
    Accession # Q86Y01
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human DTX1/DTX4 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DTX1/DTX4 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
DTX1/DTX4 in K562 Human Cell Line. DTX1/DTX4 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Intracellular Staining by Flow Cytometry
Detection of DTX1/DTX4 in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DTX1/DTX4
Deltex-1 (DTX1) is an approximately 75 kDa cytoplasmic and nuclear ubiquitin ligase that interacts with the Notch-1 intracellular domain and regulates Notch-induced gene transcription. It contains two WWE domains (aa 14-94 and aa 95-171) and one RING-type zinc finger (aa 411-472). Within aa 1-147, human Deltex-1 shares 97% aa sequence identity with mouse and rat Deltex-1.
  • Long Name:
    Deltex 1/4
  • Alternate Names:
    DTX1/DTX4
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