Detection of Human DTX1/DTX4 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DTX1/DTX4 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DTX1/DTX4 in K562 Human Cell Line.
DTX1/DTX4 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of DTX1/DTX4 in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Deltex-1 (DTX1) is an approximately 75 kDa cytoplasmic and nuclear ubiquitin ligase that interacts with the Notch-1 intracellular domain and regulates Notch-induced gene transcription. It contains two WWE domains (aa 14-94 and aa 95-171) and one RING-type zinc finger (aa 411-472). Within aa 1-147, human Deltex-1 shares 97% aa sequence identity with mouse and rat Deltex-1.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.