Human DTX1/DTX4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human DTX1/DTX4 by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human DTX1/DTX4 Antibody Summary

Species Reactivity
Detects human DTX1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human DTX4 is observed.
Monoclonal Mouse IgG2B Clone # 730612
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human DTX1/DTX4
Accession # Q86Y01
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DTX1/DTX4 antibody by Western Blot. View Larger

Detection of Human DTX1/DTX4 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DTX1/DTX4 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry DTX1/DTX4 antibody in K562 Human Cell Line by Immunocytochemistry (ICC). View Larger

DTX1/DTX4 in K562 Human Cell Line. DTX1/DTX4 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry Detection of DTX1/DTX4 antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of DTX1/DTX4 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DTX1/DTX4

Deltex-1 (DTX1) is an approximately 75 kDa cytoplasmic and nuclear ubiquitin ligase that interacts with the Notch-1 intracellular domain and regulates Notch-induced gene transcription. It contains two WWE domains (aa 14-94 and aa 95-171) and one RING-type zinc finger (aa 411-472). Within aa 1-147, human Deltex-1 shares 97% aa sequence identity with mouse and rat Deltex-1.

Long Name
Deltex 1/4
Alternate Names
DTX1/DTX4; DTX1;Fxit1;hDx-1;mKIAA4160

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Citation for Human DTX1/DTX4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The deubiquitinase Usp27x as a novel regulator of cFLIPL protein expression and sensitizer to death-receptor-induced apoptosis
    Authors: Manuel Nico Dold, Xiulin Ng, Claudia Alber, Ian Edward Gentle, Georg Häcker, Arnim Weber


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