Human E-Cadherin Antibody

Catalog # Availability Size / Price Qty
Detection of Human E‑Cadherin by Western Blot.
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Product Details
Citations (12)
Supplemental Products
Reviews (1)

Human E-Cadherin Antibody Summary

Species Reactivity
Detects human E-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cadherin-8, rhCadherin-17, recombinant mouse E-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE‑Cadherin is observed.
Monoclonal Mouse IgG2B Clone # 180215
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
Accession # P12830
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
2-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human E-Cadherin antibody by Western Blot. View Larger

Detection of Human E‑Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry E-Cadherin antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

E-Cadherin in Human Colon. E-Cadherin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) at 2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human E-Cadherin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human E‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 166 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB1838). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Cadherin

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, andL-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E-Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

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Citations for Human E-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
    Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
    International Journal of Molecular Sciences, 2022;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies
    Authors: S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain
    Ocul Surf, 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. High content screening identifies monensin as an EMT-selective cytotoxic compound
    Authors: M Vanneste, Q Huang, M Li, D Moose, L Zhao, MA Stamnes, M Schultz, M Wu, MD Henry
    Sci Rep, 2019;9(1):1200.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Modeling of Aniridia-Related Keratopathy by CRISPR/Cas9 Genome Editing of Human Limbal Epithelial Cells and Rescue by Recombinant PAX6 Protein
    Authors: LN Roux, I Petit, R Domart, JP Concordet, J Qu, H Zhou, A Joliot, O Ferrigno, D Aberdam
    Stem Cells, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/??catenin pathway
    Authors: S Zeng, B Zhu, J Zeng, W Wu, C Jiang
    Mol Med Rep, 2018;0(0):.
  6. Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages
    Authors: S Shibata, R Hayashi, T Okubo, Y Kudo, T Katayama, Y Ishikawa, J Toga, E Yagi, Y Honma, AJ Quantock, K Sekiguchi, K Nishida
    Cell Rep, 2018;25(6):1668-1679.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Rose Bengal acetate photodynamic therapy (RBAc-PDT) induces exposure and release of Damage-Associated Molecular Patterns (DAMPs) in human HeLa cells.
    Authors: Panzarini, Elisa, Inguscio, Valentin, Fimia, Gian Mar, Dini, Luciana
    PLoS ONE, 2014;9(8):e105778.
    Species: Human
    Sample Types: Cell Fraction
    Applications: Western Blot
  8. Progression of oral squamous cell carcinoma accompanied with reduced e-cadherin expression but not cadherin switch.
    Authors: Hashimoto T, Soeno Y, Maeda G, Taya Y, Aoba T, Nasu M, Kawashiri S, Imai K
    PLoS ONE, 2012;7(10):e47899.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems.
    Authors: Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB
    PLoS ONE, 2010;5(9):e13002.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Vascular endothelial growth factor-A stimulates Snail expression in breast tumor cells: implications for tumor progression.
    Authors: Wanami LS, Chen HY, Peiro S, Garcia de Herreros A, Bachelder RE
    Exp. Cell Res., 2008;314(13):2448-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  12. Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.
    Authors: Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K
    Cancer Res., 2004;64(6):2024-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


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Human E-Cadherin Antibody
By Anonymous on 10/05/2021
Application: IHC Sample Tested: Cardiac carcinoma Species: Human