Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 180215
View all E-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
Asp155-Ile707
Accession # P12830

Specificity

Detects human E-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cadherin-8, rhCadherin-17, recombinant mouse E-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE‑Cadherin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human E‑Cadherin Antibody

Detection of Human E-Cadherin antibody by Western Blot.

Detection of Human E‑Cadherin by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
E-Cadherin antibody in Human Colon by Immunohistochemistry (IHC-P).

E-Cadherin in Human Colon.

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) at 2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human E-Cadherin antibody by Simple WesternTM.

Detection of Human E‑Cadherin by Simple WesternTM.

Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 166 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB1838). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human E-Cadherin by Simple Western

Detection of Human E-Cadherin by Simple Western

D492 and D492M cells carry an epithelial and a mesenchymal phenotype, respectively. (A, B) Relative protein levels of phenotype‐specific markers in D492 and D492M cells as measured by simple western immunoassay (A; representative electropherograms, where the x‐axis shows the protein size (kDa), and the y‐axis indicates signal intensity, reflecting the amount of the protein), and the RPPA (B; average ± SD from three technical replicates). (C) Cell growth shown as increase in confluence (y‐axis) during time after seeding (x‐axis) tracked by the Incucyte (average ± SEM, n ≥ 3). (D) D492 and D492M cell colonies formed during 8‐day growth in 3D Matrigel and stained with phalloidin (red) for labeling F‐actin; scale bar, 50 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33759347), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of E-Cadherin by Western Blot

Detection of E-Cadherin by Western Blot

Targeting PCa/macrophage AR leads to increased macrophage recruitment and enhanced PCa migration through CCL2 inductionqPCR of CCL2 mRNA in THP-1 scramble (scr) and THP-1 silenced AR (siAR) cells/different PCa cell lines as indicated (left) and qPCR of CCL2 mRNA in C4-2 scr and C4-2 siAR cells (right).qPCR of CCL2 mRNA in THP-1 (scr or siAR) cells co-cultured with C4-2 scr or siAR cells (left) and in C4-2 (scr or siAR) cells co-cultured with THP-1 scr or siAR cells (right).ELISA of CCL2 in 24 h CM of C4-2 scr and C4-2 siAR cells (left) and in 24 h co-cultured CM of C4-2 scr or C4-2 siAR cells/THP-1 scr or siAR cells (right).ELISA of CCL2 in 24 h co-cultured CM of parental LNCaP cells/THP-1 scr or siAR cells (left) and in 24 h co-cultured CM of parental LAPC4 cells/THP-1 scr or siAR cells (right).Migration assay of C4-2 scr and C4-2 siAR cells incubated for 24 h (upper left), parental THP-1 cells/C4-2 scr or siAR cells after co-cultured for 16 h (upper right), parental C4-2 cells/THP-1 scr cells or siAR cells after co-cultured for 24 h (lower left), and C4-2 scr or C4-2 siAR cells/THP-1 scr or siAR cells after co-cultured for 24 h (lower right), (n = 3); bars in graphs (A–E), Mean ± SEM; bars in pictures, 400 μm (magnification 100×).Western blot of CCL2, EMT markers, AR, and PSA in parental C4-2 cells treated with CM of THP-1 scr and siAR, or co-cultured with THP-1 scr and siAR cells for 24 h (left), and in C4-2 scr and siAR cells (right). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23982944), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human E‑Cadherin Antibody

Application
Recommended Usage

Immunohistochemistry

2-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon and lung

Simple Western

5 µg/mL
Sample: A549 human lung carcinoma cell line

Western Blot

0.5 µg/mL
Sample: A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 3 reviews rated 5 using MAB1838 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Cadherin

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, andL-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E-Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

References

  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human); 12550 (Mouse); 83502 (Rat)

Gene Symbol

CDH1

UniProt

P12830

Additional E-Cadherin Products

Product Documents for Human E‑Cadherin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human E‑Cadherin Antibody

For research use only

Citations for Human E‑Cadherin Antibody

Customer Reviews for Human E‑Cadherin Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
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1 Stars
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Customer Images

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  • Human E-Cadherin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/01/2026
    Whole cell lysate of human adipocytes and MCF7 cocultureWhole cell lysate of human adipocytes and MCF7 coculture
    Human E‑Cadherin Antibody MAB1838
  • Human E-Cadherin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 01/01/2026
    Used at 0.5 ug/ml
    Human E‑Cadherin Antibody MAB1838
  • Human E-Cadherin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Cardiac carcinoma
    Species: Human
    Verified Customer | Posted 10/05/2021
    Human E‑Cadherin Antibody MAB1838

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail