Human EGFR Biotinylated Antibody

(4 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human EGFR in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse EGFR and less than 0.1% cross-reactivity with recombinant human (rh) EGF, rhErbB2, and rhErbB3.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human EGFR
    Leu25-Ser645
    Accession # CAA25240
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human EGFR (Catalog # 1095-ER)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
    • Human EGFR Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human EGFR Antibody (Catalog # AF231)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human EGFR Biotinylated Antibody (Catalog # BAF231)
  • ELISA Standard
     
    Recombinant Human EGFR Protein (Catalog # 1095-ER)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of EGFR in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human EGFR Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF231, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGFR
The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha  in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis (1-3).
  • References:
    1. Daly, R.J. (1999) Growth Factors, 16:255.
    2. Schlessinger, J. (2000) Cell. 103:211.
    3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
     
  • Long Name:
    Epidermal Growth Factor Receptor
  • Entrez Gene IDs:
    1956 (Human); 13649 (Mouse)
  • Alternate Names:
    avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Species
Applications
Sample Type
  1. Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1.
    Authors: Sakanyan V, Hulin P, Alves de Sousa R, Silva V, Hambardzumyan A, Nedellec S, Tomasoni C, Loge C, Pineau C, Roussakis C, Fleury F, Artaud I
    Sci Rep, 2016;6(0):21088.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Protein shedding in urothelial bladder cancer: prognostic implications of soluble urinary EGFR and EpCAM.
    Authors: Bryan R, Regan H, Pirrie S, Devall A, Cheng K, Zeegers M, James N, Knowles M, Ward D
    Br J Cancer, 2015;112(6):1052-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR.
    Authors: Thompson C, Bloom L, Ogiue-Ikeda M, Machida K
    BMC Biotechnol, 2015;15(0):60.
    Species: Human
    Sample Type: Cell Lysates
    Application: Functional Assay
  4. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Type: Serum
    Application: ELISA Microarray Development
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Allophycocyanin

Flow F0050 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Avidin-Fluorescein

Flow F0030 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

MagCellect Streptavidin Ferrofluid

MAG999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates

ELISA CP004
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4 (Based on 1 review)

Have you used Human EGFR Biotinylated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human EGFR Biotinylated Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

Filter by Ratings
Very Good
(1)
All Ratings
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Very Good
 ELISA Human Anonymous 02/22/2016
Show

Summary

ApplicationELISA
Sample Testedunknown
SpeciesHuman

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Associated Pathways

Feedback

Customer Information