Human EGFR Antibody [Biotin]

R&D Systems | Catalog # BAF231

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Flow Cytometry

Cited:

Western Blot, Flow Cytometry, ELISA Detection, ELISA Microarray Development, Functional Assay

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EGFR
Leu25-Ser645
Accession # CAA25240

Specificity

Detects human EGFR in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse EGFR and less than 0.1% cross-reactivity with recombinant human (rh) EGF, rhErbB2, and rhErbB3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human EGFR Antibody [Biotin]

Detection of EGFR antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of EGFR in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Human EGFR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF231, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).
Detection of EGFR by Western Blot

Detection of EGFR by Western Blot

Applications of SH2-PLA assay. d, Application of SH2-PLA for cancer tissue analysis. The SH2-PLA/Western/far-Western analyses were performed using 10 lung cancer tissue samples. Upper panel shows Western and far-Western results with antibody/probe names indicated on the left. Only one sample (#3) shows an EGFR size band which also overlapped with bands detected by anti-pTyr and Grb2 SH2 (far-Western image represents 60-min exposure). The tyrosine phosphorylation level of the band is similar to the weak phosphorylation of EGFR in unstimulated A431 cells (right panel). The PLA-SH2 results for the same set of lung cancer samples are shown on the bottom. Consistent with the Grb2 far-Western result, only sample #3 had significant signal beyond the no protein control (NPC). The BG line indicates the background Ct value Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26112401), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human EGFR ELISA Standard Curve

Human EGFR ELISA Standard Curve

Recombinant Human EGFR (Catalog # 1095-ER) was serially diluted and captured by Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human EGFR Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human EGFR (Catalog # 1095-ER)

Human EGFR Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human EGFR Antibody (Catalog # AF231)
  • Standard: Recombinant Human EGFR Protein (Catalog # 1095-ER)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using BAF231 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGFR

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha  in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis (1-3).

References

  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
 

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

CAA25240

Additional EGFR Products

Product Documents for Human EGFR Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EGFR Antibody [Biotin]

For research use only

Citations for Human EGFR Antibody [Biotin]

Customer Reviews for Human EGFR Antibody [Biotin] (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human EGFR Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 03/17/2021
    Human EGFR Antibody [Biotin] BAF231
  • Human EGFR Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/08/2018
  • Human EGF R/ErbB1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: unknown
    Species: Human
    Verified Customer | Posted 02/22/2016

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