Human Endocan/ESM-1 Antibody

Catalog # Availability Size / Price Qty
AF1810
AF1810-SP
Detection  of Human Endocan/ESM‑1 by Western Blot.
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Product Details
Citations (3)
FAQs
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Human Endocan/ESM-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endocan in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Endocan is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endocan/ESM-1
Trp20-Arg184
Accession # Q9NQ30
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Neutralization
Measured by its ability to neutralize Endocan/ESM‑1-mediated adhesion of the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 20 µg/mL Recombinant Human Endocan/ESM‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human Endocan/ESM‑1 by Western Blot. View Larger

Detection of Human Endocan/ESM‑1 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endocan/ESM‑1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Cell Adhesion Mediated by Endocan/ESM‑1 and Neutral­ization by Human Endocan/ESM‑1 Antibody. View Larger

Cell Adhesion Mediated by Endocan/ESM‑1 and Neutral­ization by Human Endocan/ESM‑1 Antibody. Recombinant Human Endocan/ESM‑1 (Catalog # 1810‑EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Endocan/ESM‑1 (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810). The ND50 is typically 1-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endocan/ESM-1

Endocan, also known as endothelial-cell specific molecule-1 (ESM-1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung (1). Endocan expression has also been detected in different epithelia and in adipocytes (2, 3). The expression of endocan is upregulated by TNF‑ alpha, IL-1 beta, or lipopolysaccharide and down-regulated by IFN‑ gamma (1). The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues (1). The DS chain is covalently attached to serine 137 (4). Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen-1, LFA-1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA-1-mediated leukocyte activation (5). Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity (3, 6). Genetically engineered cells overexpressing endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo (3, 6). Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported (2, 6).

References
  1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
  2. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
  3. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217.
  4. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
  5. Bechard, D. et al. (2001) J. Immunol. 167:3099
  6. Scherpereel, A. et al. (2003) Cancer Res. 63:6084.
Entrez Gene IDs
11082 (Human); 71690 (Mouse)
Alternate Names
Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6

Product Datasheets

Citations for Human Endocan/ESM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Upregulation of endocan by Epstein-Barr virus latent membrane protein 1 and its clinical significance in nasopharyngeal carcinoma.
    Authors: Yu P, Chou S, Chen C, Hung H, Lai C, Yang P, Jeng Y, Liaw S, Kuo H, Hsu H, Chen J, Wang W
    PLoS ONE, 2013;8(12):e82254.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  2. Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis.
    Authors: Shin JW, Huggenberger R, Detmar M
    Blood, 2008;112(6):2318-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Endocan is a VEGF-A and PI3K regulated gene with increased expression in human renal cancer.
    Authors: Rennel E, Mellberg S, Dimberg A, Petersson L, Botling J, Ameur A, Westholm JO, Komorowski J, Lassalle P, Cross MJ, Gerwins P
    Exp. Cell Res., 2007;313(7):1285-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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