Human Endocan/ESM-1 Antibody

R&D Systems | Catalog # AF1810

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunoprecipitation, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Endocan/ESM-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Endocan/ESM-1
Trp20-Arg184
Accession # Q9NQ30

Specificity

Detects human Endocan in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Endocan is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Endocan/ESM-1 Antibody

Detection of Human Endocan/ESM-1 antibody by Western Blot.

Detection of Human Endocan/ESM‑1 by Western Blot.

Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endocan/ESM-1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cell Adhesion Mediated by Endocan/ESM‑1 and Neutral-ization by Human Endocan/ESM‑1 Antibody.

Cell Adhesion Mediated by Endocan/ESM‑1 and Neutral-ization by Human Endocan/ESM‑1 Antibody.

Recombinant Human Endocan/ESM-1 (Catalog # 1810-EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Endocan/ESM-1 (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810). The ND50 is typically 1-4 µg/mL.

Applications for Human Endocan/ESM-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Neutralization

Measured by its ability to neutralize Endocan/ESM‑1-mediated adhesion of the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 20 µg/mL Recombinant Human Endocan/ESM‑1.

Reviewed Applications

Read 1 review rated 4 using AF1810 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Endocan/ESM-1

Endocan, also known as endothelial-cell specific molecule-1 (ESM-1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung (1). Endocan expression has also been detected in different epithelia and in adipocytes (2, 3). The expression of endocan is upregulated by TNF‑ alpha, IL-1 beta, or lipopolysaccharide and down-regulated by IFN‑ gamma (1). The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues (1). The DS chain is covalently attached to serine 137 (4). Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen-1, LFA-1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA-1-mediated leukocyte activation (5). Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity (3, 6). Genetically engineered cells overexpressing endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo (3, 6). Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported (2, 6).

References

  1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
  2. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
  3. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217.
  4. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
  5. Bechard, D. et al. (2001) J. Immunol. 167:3099
  6. Scherpereel, A. et al. (2003) Cancer Res. 63:6084.

Alternate Names

ESM-1, ESM1, IGFBP-rp6

Entrez Gene IDs

11082 (Human); 71690 (Mouse)

Gene Symbol

ESM1

UniProt

Q9NQ30

Additional Endocan/ESM-1 Products

Product Documents for Human Endocan/ESM-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Endocan/ESM-1 Antibody

For research use only

Citations for Human Endocan/ESM-1 Antibody

Customer Reviews for Human Endocan/ESM-1 Antibody (1)

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  • Human Endocan/ESM-1 Antibody
    Name: Anuj Ahuja
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 06/09/2022
    Human Endocan/ESM-1 Antibody AF1810

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