Human eNOS Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF950
Product Details
Citations (1)
FAQs
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Human eNOS Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human eNOS.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human eNOS peptide
aa 1030-1203
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human eNOS
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eNOS

Nitric oxide (NO) is a pleiotropic signaling molecule implicated in diverse biological processes including inhibition of platelet aggregation, regulation of neurotransmission, vasodilation, immune responses, and inflammation. NO is synthesized from L-arginine and O2 by three nitric oxide synthase (NOS) enzymes termed endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS). Each enzyme isoform is expressed in a variety of tissues and cell types. While eNOS and nNOS generally exhibit constitutive expression and are involved in physiological signaling and maintenance functions, iNOS expression is induced by inflammatory stimuli and is associated with both normal and pathological immune responses.

Long Name
Endothelial Nitric Oxide Synthase
Entrez Gene IDs
4846 (Human); 18127 (Mouse)
Alternate Names
Constitutive NOS; EC 1.14.13.39; ECNOS; Endothelial NOS; endothelial; eNOS; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII

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Citation for Human eNOS Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neointimal hyperplasia on a cell-seeded polytetrafluoroethylene graft is promoted by transfer of tissue plasminogen activator gene and inhibited by transfer of nitric oxide synthase gene.
    Authors: Yu H, Dai W, Yang Z, Romaguera RL, Kirkman P, Rowe VL
    J. Vasc. Surg., 2005;41(1):122-9.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P

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