Detects human EphA1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) EphA3, rmEphA8, rmEphA4, rmEphA2, rmEphA6, rmEphA7, and recombinant rat EphA5 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human EphA1 Lys26-Glu547 Accession # P21709
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of EphA1 in MCF‑7 Human Cell Line by Flow Cytometry.
MCF‑7 human breast cancer cell line was stained with Goat Anti-Human EphA1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB638P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of EphA1 in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Goat Anti-Human EphA1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB638P) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
EphA1, also known as Eph and Esk (1), is a homodimeric member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues that are major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail that contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA1 has been shown to bind ephrin-A1 (2, 3). The extracellular domains of mouse and human EphA1 share greater than 91% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
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