Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

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DEPRU0
Human Epo ELISA Benchtop Protocol Standard Curve
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Human Erythropoietin/EPO Quantikine ELISA Kit, RUO Summary

Assay Length
4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol)
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL)
Sensitivity
0.6 mIU/mL
Assay Range
2.5 - 200 mIU/mL (Serum, EDTA Plasma)
Specificity
Natural and recombinant human Epo.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with other substances.

Product Summary

The Quantikine® Human Epo ELISA uses a monoclonal antibody and polyclonal antibody conjugate in a sandwich ELISA format. The assay is designed to measure Epo levels in serum or EDTA plasma in less than 4.5 hours, or less than 2.5 hours using the shaker protocol.

Precision

Benchtop Protocol Precision (Precision within an assay) Four samples of known concentration were tested thirty times to assess intra-assay precision
Shaker Protocol Precision (Precision between assays) Four samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components

Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3 4
n 30 30 30 30 30 30 30 30
Mean (mIU/mL) 5.82 16.01 25.18 79.02 6.45 16.22 26.35 84.14
Standard Deviation 0 0 0 0 0 0 0 0
CV% 8.25 4.3 5.9 4.24 10.3 3.74 4.2 3.71

Recovery

The recovery of human Epo spiked to levels throughout the range of the assay was evaluated in 10 serum and plasma samples.                                                                                                                                                                                          

           

Specimen Type       

           

Protocol       

           

Amount Needed       

           

Mean Recovery       

SerumBenchtop52.2 mlU/mL100%
Shaker57.5 mlU/mL93%
Plasma (EDTA)Benchtop51.8 mlU/mL102%
Shaker55.9 mlU/mL105%

Linearity

Five matched serum and plasma specimens containing elevated Epo concentrations were diluted with Specimen Diluent and assayed using the Quantikine Human Epo ELISA. Diluted specimens demonstrated very good linearity when compared to neat concentrations of Epo.
Human Epo ELISA Benchtop Protocol Linearity
Human Epo ELISA Shaker Protocol Linearity

Data Examples

Human Epo ELISA Benchtop Protocol Standard Curve

Human Epo ELISA Shaker Protocol Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Erythropoietin/EPO

Erythropoietin (Epo), a glycoprotein (~30,400 Daltons) produced primarily by the kidney, is the principal factor regulating red blood cell production (erythropoiesis) in mammals. Renal production of Epo is regulated by changes in oxygen availability. Under conditions of hypoxia, the level of Epo in the circulation increases and this leads to increased production of red blood cells. 

The over-expression of Epo may be associated with certain pathophysiological conditions (1, 2). Polycythemia exists when there is an overproduction of red blood cells (RBCs). Primary polycythemias, such as polycythemia vera, are caused by Epo-independent growth of erythrocytic progenitors from abnormal stem cells and low to normal levels of Epo are found in the serum of affected patients. On the other hand, various types of secondary polycythemias are associated with the production of higher than normal levels of Epo. The overproduction of Epo may be an adaptive response associated with conditions that produce tissue hypoxia, such as living at high altitude, chronic obstructive pulmonary disease, cyanotic heart disease, sleep apnea, high-affinity hemoglobinopathy, smoking, or localized renal hypoxia (1, 2). In other instances, excessive Epo levels are the result of production by neoplastic cells. Cases of increased Epo production and erythrocytosis have been reported for patients with renal carcinomas (3), benign renal tumors (4), Wilms' tumors, hepatomas (5), liver carcinomas (6), cerebellar hemangioblastomas (3, 7, 8), adrenal gland tumors (9), smooth muscle tumors (3, 9), and leiomyomas (10). 
Deficient Epo production is found in conjunction with certain forms of anemias. These include anemia of renal failure and end-stage renal disease (1, 2, 11), anemias of chronic disorders [chronic infections (1), autoimmune diseases (1), rheumatoid arthritis (12), AIDS (13), malignancies (14)], anemia of prematurity (2), anemia of hypothyroidism (2), and anemia of malnutrition (2). Many of these conditions are associated with the generation of IL-1 and TNF-alpha, factors that have been shown to be inhibitors of Epo activity (1, 15). Other forms of anemias, on the other hand, are due to Epo-independent causes and affected individuals show elevated levels of Epo (2). These forms include aplastic anemias, iron deficiency anemias, thalassemias, megaloblastic anemias, pure red cell aplasias, and myelodysplastic syndromes.

Entrez Gene IDs:
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names:
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citation for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
    Authors: PH Jaïs, E Decroly, E Jacquet, M Le Boulch, A Jaïs, O Jean-Jean, H Eaton, P Ponien, F Verdier, B Canard, S Goncalves, S Chiron, M Le Gall, P Mayeux, M Shmulevitz
    Nucleic Acids Res., 2019;0(0):.
    Species: Transgenic Hamster
    Sample Types: Cell Culture Supernates

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Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
By Anonymous on 11/22/2021
Application: Sample Tested: human floxp embryonal stem cell culture medium

Human Erythropoietin Quantikine ELISA Kit, RUO
By Anonymous on 04/13/2018
Application: Sample Tested: Serum