Human Erythropoietin Quantikine ELISA Kit, RUO

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DEPRU0
Human Epo ELISA Benchtop Protocol Standard Curve
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Human Erythropoietin Quantikine ELISA Kit, RUO Summary

Assay Length
4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol)
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL)
Sensitivity
0.6 mIU/mL
Assay Range
2.5 - 200 mIU/mL (Serum, EDTA Plasma)
Specificity
Natural and recombinant human Epo.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with other substances.

Product Summary

The Quantikine® Human Epo ELISA uses a monoclonal antibody and polyclonal antibody conjugate in a sandwich ELISA format. The assay is designed to measure Epo levels in serum or EDTA plasma in less than 4.5 hours, or less than 2.5 hours using the shaker protocol.

Precision

Benchtop Protocol Precision (Precision within an assay) Four samples of known concentration were tested thirty times to assess intra-assay precision
Shaker Protocol Precision (Precision between assays) Four samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components

Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3 4
n 30 30 30 30 30 30 30 30
Mean 5.82 16.01 25.18 79.02 6.45 16.22 26.35 84.14
Standard Deviation 0 0 0 0 0 0 0 0
CV% 8.25 4.3 5.9 4.24 10.3 3.74 4.2 3.71

Recovery

The recovery of human Epo spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Serum (n=)

Linearity

Five matched serum and plasma specimens containing elevated Epo concentrations were diluted with Specimen Diluent and assayed using the Quantikine Human Epo ELISA. Diluted specimens demonstrated very good linearity when compared to neat concentrations of Epo.
Human Epo ELISA Benchtop Protocol Linearity
Human Epo ELISA Shaker Protocol Linearity

Data Examples

Human Epo ELISA Benchtop Protocol Standard Curve

Human Epo ELISA Shaker Protocol Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Erythropoietin

Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

Entrez Gene IDs:
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names:
EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

FAQs

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Human Erythropoietin Quantikine ELISA Kit, RUO
By Anonymous on 04/13/2018
Sample Tested: Serum