Human Erythropoietin Quantikine IVD ELISA Kit

For In Vitro Diagnostic Use
  (52 citations)
(4 Reviews)
    
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (52)
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol)
  • Sample Type & Volume Required Per Well
    Serum (100 uL), EDTA Plasma (100 uL)
  • Sensitivity
    0.6 mIU/mL
  • Assay Range
    2.5 - 200 mIU/mL (Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant human Epo
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with other substances.
Control Available
CEP01 , Human Erythropoietin Quantikine IVD Control 1&2 (5 vials ea) - Please Inquire
CEP03 , Human Erythropoietin Quantikine IVD Control 3 (10 vials) - Please Inquire
Product Summary
The Quantikine® IVD Human Epo ELISA is designed to measure Epo levels in serum or plasma in less than 4.5 hours (or less than 2.5 hours using the shaker protocol) for the quantitative determination of erythropoietin (Epo) concentration in human serum and plasma as an aid in the diagnosis of anemia and polycythemia. For in vitro diagnostic use.

Precision
Precision within an assay: (Benchtop Protocol) Four control pools were tested on one plate to assess intra-assay precision. Thirty assays were run using each protocol over a thirty day period with three separately manufactured lots of reagents
Precision between assays: (Shaker Protocol) Four control pools were tested in separate assays to assess inter-assay precision. Thirty assays were run using each protocol over a thirty day period with three separately manufactured lots of reagents
Serum, EDTA Plasma
Benchtop Protocol Precision Shaker Protocol Precision
Sample 1 2 3 4 1 2 3 4
n 30 30 30 30 30 30 30 30
Mean 5.82 16.01 25.18 79.02 6.45 16.22 26.35 84.14
Standard Deviation 0 0 0 0 0 0 0 0
CV% 4.95 2.84 5.22 3.06 7.82 3.02 2.18 2.42

Recovery

The recovery of human Epo spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (Benchtop Protocol) (n=10) 102
EDTA Plasma (Shaker Protocol) (n=10) 105
Serum (Benchtop Protocol) (n=10) 100
Serum (Shaker Protocol) (n=10) 93
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Epo were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Erythropoietin [HRP]
 Erythropoietin [HRP]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Erythropoietin
Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

  • Entrez Gene IDs:
    2056 (Human); 13856 (Mouse); 24335 (Rat)
  • Alternate Names:
    EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and specimens to room temperature before use. It is recommended that all specimens, standards, and controls be assayed in duplicate. Benchtop and shaker protocols are provided. The same protocol must be used throughout the assay.
  1.   Prepare all reagents as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Specimen
  6.   Add 100 µL of Standard, control, or specimen to each well. Cover with a plate sealer.
  7.   For Benchtop Protocol: Incubate at room temperature for 2 hours.
    For Shaker Protocol: Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  8.   Thoroughly aspirate each well. Do not wash.

  9. 200 µL Conjugate
  10.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer.
  11.   For Benchtop Protocol: Incubate at room temperature for 2 hours.
    For Shaker Protocol: Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  12.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  13. 200 µL Substrate Solution
  14. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20-25 minutes on the benchtop.

  15. 100 µL Stop Solution
  16. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 600 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

52 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Successful reprogramming of cellular protein production through mRNA delivered by functionalized lipid nanoparticles
    Authors: M Yanez Arte, T Kjellman, S Bartesaghi, S Wallin, X Wu, AJ Kvist, A Dabkowska, N Székely, A Radulescu, J Bergenholt, L Lindfors
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. Recombinant erythropoietin acutely decreases renal perfusion and decouples the renin-angiotensin-aldosterone system
    Authors: NJ Aachmann-A, SJ Christense, K Lisbjerg, P Oturai, PI Johansson, NH Holstein-R, NV Olsen
    Physiol Rep, 2018;6(5):.
    Species: Human
    Sample Type: Plasma
  3. Hemolytic anemia repressed hepcidin level without hepatocyte iron overload: lesson from G�nther disease model
    Authors: S Millot, C Delaby, B Moulouel, T Lefebvre, N Pilard, N Ducrot, C Ged, P Lett‚ron, L de Frances, JC Deybach, C Beaumont, L Gouya, H De Verneui, S Lyoumi, H Puy, Z Karim
    Haematologica, 2017;102(2):260-270.
    Species: Human
    Sample Type: Serum
  4. Renal Handling of Ketones in Response to Sodium-Glucose Cotransporter 2 Inhibition in Patients With Type 2 Diabetes
    Authors: E Ferrannini, S Baldi, S Frascerra, B Astiarraga, E Barsotti, A Clerico, E Muscelli
    Diabetes Care, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  5. Anaemia in hospitalised preschool children from a rural area in Mozambique: a case control study in search for aetiological agents
    Authors: C Moraleda, R Aguilar, L Quintó, T Nhampossa, M Renom, A Nhabomba, S Acácio, JJ Aponte, D Nhalungo, AH Achtman, L Schofield, H Martins, E Macete, PL Alonso, C Menéndez
    BMC Pediatr, 2017;17(1):63.
    Species: Human
    Sample Type:
  6. Erythropoietin Slows Photoreceptor Cell Death in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa
    Authors: Tonia S Rex
    PLoS ONE, 2016;11(6):e0157411.
    Species: Mouse
    Sample Type: Serum
  7. PlanHab: Hypoxia counteracts the erythropoietin suppression, but seems to exaggerate the plasma volume reduction induced by 3�weeks of bed rest
    Authors: ME Keramidas, IB Mekjavic, R Kölegård, A Choukèr, C Strewe, O Eiken
    Physiol Rep, 2016;4(7):.
    Species: Human
    Sample Type: Serum
  8. Diurnal changes of arterial oxygen saturation and erythropoietin concentration in male and female highlanders
    Physiol Rep, 2016;4(17):.
    Species: Human
    Sample Type: Plasma
  9. Improved Human Erythropoiesis and Platelet Formation in Humanized NSGW41 Mice
    Stem Cell Reports, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  10. Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system
    Gene Ther., 2016;23(10):699-707.
    Species: Human
    Sample Type: Serum
  11. Revisiting Biomarkers of Total-Body and Partial-Body Exposure in a Baboon Model of Irradiation.
    Authors: Valente M, Denis J, Grenier N, Arvers P, Foucher B, Desangles F, Martigne P, Chaussard H, Drouet M, Abend M, Herodin F
    PLoS ONE, 2015;10(7):e0132194.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Type: Plasma
  12. Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
    Authors: de Lucas Cerrillo A, Bond W, Rex T
    Gene Ther, 2015;22(5):365-73.
    Species: Hamster
    Sample Type: Cell Culture Supernates
  13. Sequence-engineered mRNA Without Chemical Nucleoside Modifications Enables an Effective Protein Therapy in Large Animals.
    Authors: Thess A, Grund S, Mui B, Hope M, Baumhof P, Fotin-Mleczek M, Schlake T
    Mol Ther, 2015;23(9):1456-64.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Serum
  14. Mimicking hypoxia to treat anemia: HIF-stabilizer BAY 85-3934 (Molidustat) stimulates erythropoietin production without hypertensive effects.
    Authors: Flamme I, Oehme F, Ellinghaus P, Jeske M, Keldenich J, Thuss U
    PLoS ONE, 2014;9(11):e111838.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Plasma
  15. Erythropoietin rs1617640 G allele associates with an attenuated rise of serum erythropoietin and a marked decline of hemoglobin in hepatitis C patients undergoing antiviral therapy.
    Authors: Amanzada A, Goralczyk A, Reinhardt L, Moriconi F, Cameron S, Mihm S
    BMC Infect Dis, 2014;14(0):503.
    Species: Human
    Sample Type: Serum
  16. Hypoxic response contributes to altered gene expression and precapillary pulmonary hypertension in patients with sickle cell disease.
    Authors: Zhang X, Zhang W, Ma S, Desai A, Saraf S, Miasniakova G, Sergueeva A, Ammosova T, Xu M, Nekhai S, Abbasi T, Casanova N, Steinberg M, Baldwin C, Sebastiani P, Prchal J, Kittles R, Garcia J, Machado R, Gordeuk V
    Circulation, 2014;129(16):1650-8.
    Species: Human
    Sample Type: Serum
  17. The relation of hepcidin to iron disorders, inflammation and hemoglobin in chronic kidney disease.
    Authors: Mercadel L, Metzger M, Haymann J, Thervet E, Boffa J, Flamant M, Vrtovsnik F, Houillier P, Froissart M, Stengel B
    PLoS ONE, 2014;9(6):e99781.
    Species: Human
    Sample Type: Serum
  18. Hematological parameters, and hematopoietic growth factors: EPO and IL-3 in response to whole-body cryostimulation (WBC) in military academy students.
    Authors: Szygula Z, Lubkowska A, Giemza C, Skrzek A, Bryczkowska I, Dolegowska B
    PLoS ONE, 2014;9(4):e93096.
    Species: Human
    Sample Type: Plasma
  19. Targeted delivery of erythropoietin by transcranial focused ultrasound for neuroprotection against ischemia/reperfusion-induced neuronal injury: a long-term and short-term study.
    Authors: Wu, Sheng-Ka, Yang, Ming-Tao, Kang, Kai-Hsia, Liou, Houng-Ch, Lu, Dai-Hua, Fu, Wen-Mei, Lin, Win-Li
    PLoS ONE, 2014;9(2):e90107.
    Species: Rat
    Sample Type: Tissue Homogenates
  20. The effect of the neonatal Fc receptor on human IgG biodistribution in mice.
    Authors: Chen, Nancy, Wang, Weirong, Fauty, Scott, Fang, Yulin, Hamuro, Lora, Hussain, Azher, Prueksaritanont, Thomayan
    MAbs, 2014;6(2):502-8.
    Species: Mouse
    Sample Type: Plasma
  21. Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease.
    Authors: Krautkramer E, Grouls S, Hettwer D, Rafat N, Tonshoff B, Zeier M
    J Virol, 2014;88(1):483-9.
    Species: Human
    Sample Type: Plasma
  22. Transgene regulation using the tetracycline-inducible TetR-KRAB system after AAV-mediated gene transfer in rodents and nonhuman primates.
    Authors: Le Guiner C, Stieger K, Toromanoff A, Guilbaud M, Mendes-Madeira A, Devaux M, Guigand L, Cherel Y, Moullier P, Rolling F, Adjali O
    PLoS ONE, 2014;9(9):e102538.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Serum
  23. Bone marrow mesenchymal stem cells in patients with beta thalassemia major: molecular analysis with attenuated total reflection-Fourier transform infrared spectroscopy study as a novel method.
    Stem Cells Dev., 2012;21(11):2000-11.
    Species: Human
    Sample Type: Bone Marrow Plasma
  24. Longevity of rAAV vector and plasmid DNA in blood after intramuscular injection in nonhuman primates: implications for gene doping.
    Authors: Ni W, Le Guiner C, Gernoux G
    Gene Ther., 2011;18(7):709-18.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Serum
  25. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
    Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
    Blood, 2011;117(13):3485-93.
    Species: Human
    Sample Type: Plasma
  26. Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
    Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
    J. Cardiothorac. Vasc. Anesth., 2009;23(3):316-23.
    Species: Human
    Sample Type: Plasma
  27. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
    Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
    Exp. Hematol., 2009;37(6):673-8.
    Species: Human
    Sample Type: Plasma
  28. Influence of inflammation as measured by alpha-1-acid glycoprotein on iron status indicators among HIV-positive postpartum Zimbabwean women.
    Authors: Rawat R, Stoltzfus RJ, Ntozini R, Mutasa K, Iliff PJ, Humphrey JH
    Eur J Clin Nutr, 2009;63(6):787-93.
    Species: Human
    Sample Type: Serum
  29. Alterations of systemic and muscle iron metabolism in human subjects treated with low-dose recombinant erythropoietin.
    Authors: Robach P, Recalcati S, Girelli D, Gelfi C, Aachmann-Andersen NJ, Thomsen JJ, Norgaard AM, Alberghini A, Campostrini N, Castagna A, Vigano A, Santambrogio P, Kempf T, Wollert KC, Moutereau S, Lundby C, Cairo G
    Blood, 2009;113(26):6707-15.
    Species: Human
    Sample Type: Plasma
  30. Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
    Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
    2008;84(0):481.
    Species: Human
    Sample Type: Plasma
  31. Iron metabolism in heterozygotes for hemoglobin E (HbE), alpha-thalassemia 1, or beta-thalassemia and in compound heterozygotes for HbE/beta-thalassemia.
    Authors: Zimmermann MB, Fucharoen S, Winichagoon P, Sirankapracha P, Zeder C, Gowachirapant S, Judprasong K, Tanno T, Miller JL, Hurrell RF
    Am. J. Clin. Nutr., 2008;88(4):1026-31.
    Species: Human
    Sample Type: Serum
  32. Role of Gas6 in erythropoiesis and anemia in mice.
    Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
    J. Clin. Invest., 2008;118(2):583-96.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  33. Pulmonary arterial hypertension and left-sided heart disease in sickle cell disease: clinical characteristics and association with soluble adhesion molecule expression.
    Authors: Klings ES, Anton Bland D, Rosenman D, Princeton S, Odhiambo A, Li G, Bernard SA, Steinberg MH, Farber HW
    Am. J. Hematol., 2008;83(7):547-53.
    Species: Human
    Sample Type: Plasma
  34. Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure.
    Authors: Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM
    J. Am. Coll. Cardiol., 2007;49(24):2341-9.
    Species: Human
    Sample Type: Serum
  35. Increased circulating endothelial progenitor cells in septic patients: correlation with survival.
    Authors: Rafat N, Hanusch C, Brinkkoetter PT, Schulte J, Brade J, Zijlstra JG, van der Woude FJ, van Ackern K, Yard BA, Beck GCh
    Crit. Care Med., 2007;35(7):1677-84.
    Species: Human
    Sample Type: Serum
  36. Strong iron demand during hypoxia-induced erythropoiesis is associated with down-regulation of iron-related proteins and myoglobin in human skeletal muscle.
    Authors: Robach P, Cairo G, Gelfi C, Bernuzzi F, Pilegaard H, Vigano A, Santambrogio P, Cerretelli P, Calbet JA, Moutereau S, Lundby C
    Blood, 2007;109(11):4724-31.
    Species: Human
    Sample Type: Serum
  37. Cerebrospinal fluid erythropoietin (EPO) in amyotrophic lateral sclerosis.
    Authors: Brettschneider J, Widl K, Schattauer D, Ludolph AC, Tumani H
    Neurosci. Lett., 2007;416(3):257-60.
    Species: Human
    Sample Type: Serum
  38. Oral administration of doxycycline allows tight control of transgene expression: a key step towards gene therapy of retinal diseases.
    Authors: Stieger K, Mendes-Madeira A, Meur GL, Weber M, Deschamps JY, Nivard D, Provost N, Moullier P, Rolling F
    Gene Ther., 2007;14(23):1668-73.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Aqueous humor
  39. Progress toward a nonviral gene therapy protocol for the treatment of anemia.
    Authors: Sebestyen MG, Hegge JO, Noble MA, Lewis DL, Herweijer H, Wolff JA
    Hum. Gene Ther., 2007;18(3):269-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Serum
  40. Long-term doxycycline-regulated transgene expression in the retina of nonhuman primates following subretinal injection of recombinant AAV vectors.
    Authors: Stieger K, Le Meur G, Lasne F, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Martin L, Moullier P, Rolling F
    Mol. Ther., 2006;13(5):967-75.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Vitreous Humor
  41. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
    Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
    Am. J. Respir. Crit. Care Med., 2006;174(1):51-7.
    Species: Human
    Sample Type: Plasma
  42. Effects of chronic hypoxia in vivo on the expression of human placental glucose transporters.
    Authors: Zamudio S, Baumann MU, Illsley NP
    Placenta, 2006;27(1):49-55.
    Species: Human
    Sample Type: Serum
  43. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
    Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
    Br. J. Cancer, 2006;94(4):524-31.
    Species: Human
    Sample Type: Serum
  44. Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
    Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
    Exp. Hematol., 2006;34(10):1312-22.
    Species: Human
    Sample Type: Serum
  45. Long-term pharmacologically regulated expression of erythropoietin in primates following AAV-mediated gene transfer.
    Authors: Rivera VM, Gao GP, Grant RL, Schnell MA, Zoltick PW, Rozamus LW, Clackson T, Wilson JM
    Blood, 2005;105(4):1424-30.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Serum
  46. Haemoglobin E beta thalassaemia in Sri Lanka.
    Authors: Premawardhena A, Fisher CA, Olivieri NF, De Silva S, Arambepola M, Perera W, O&amp;amp;apos;Donnell A, O'Donnell A, O'Donnell A, O'Donnell A, O'Donnell A, Peto TE, Viprakasit V, Merson L, Muraca G, Weatherall DJ
    Lancet, 2005;366(9495):1467-70.
    Species: Human
    Sample Type: Serum
  47. Monoclonal myelopoiesis and subnormal erythropoietin concentration are independent risk factors for thromboembolic complications in essential thrombocythemia.
    Authors: Andreasson B, Harrison C, Lindstedt G, Linch D, Kutti J
    Blood, 2003;101(2):783.
    Species: Human
    Sample Type: Serum
  48. Expansion of human SCID-repopulating cells under hypoxic conditions.
    Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
    J. Clin. Invest., 2003;112(1):126-35.
    Species: Human
    Sample Type: Cell Culture Supernates
  49. Erythropoietin therapy for acute stroke is both safe and beneficial.
    Authors: Ehrenreich H, Hasselblatt M, Dembowski C, Cepek L, Lewczuk P, Stiefel M, Rustenbeck HH, Breiter N, Jacob S, Knerlich F, Bohn M, Poser W, Ruther E, Kochen M, Gefeller O, Gleiter C, Wessel TC, De Ryck M, Itri L, Prange H, Cerami A, Brines M, Siren AL
    Mol. Med., 2002;8(8):495-505.
    Species: Human
    Sample Type: Serum
  50. Evaluation of tumor necrosis factor-alpha and erythropoietin serum levels in B-cell chronic lymphocytic leukemia patients with anemia.
    Authors: Capalbo S, Battista C, Delia M, Ciancio A, De Santis G, Dargenio M, Diomede D, Liso V
    Acta Haematol., 2002;108(2):84-9.
    Species: Human
    Sample Type: Serum
  51. Lack of an immune response against the tetracycline-dependent transactivator correlates with long-term doxycycline-regulated transgene expression in nonhuman primates after intramuscular injection of recombinant adeno-associated virus.
    Authors: Favre D, Blouin V, Provost N, Spisek R, Porrot F, Bohl D, Marme F, Cherel Y, Salvetti A, Hurtrel B, Heard JM, Riviere Y, Moullier P
    J. Virol., 2002;76(22):11605-11.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Serum
  52. Adeno-associated virus-mediated delivery of erythropoietin leads to sustained elevation of hematocrit in nonhuman primates.
    Authors: Zhou S, Murphy JE, Escobedo JA, Dwarki VJ
    Gene Ther., 1998;5(5):665-70.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Serum
Expand to show all Citations

FAQs

  1. What type of EPO is used in the standard for the Human Erythropoietin Quantikine® IVD® ELISA kit (Catalog # DEP00)?

    • The standard in this kit contains CHO (Chinese Hamster Ovary) cell-derived Recombinant Human EPO.

  2. Can the Human Erythropoietin Quantikine® IVD® ELISA kit (Catalog # DEP00) be used with heparin plasma?

    • Heparin plasma samples are not validated nor recommended for use in the Human Erythropoietin Quantikine® IVD® ELISA kit. During kit validation, results of in-house testing did not meet our specifications.

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Human Erythropoietin Quantikine IVD Control 1&2 (5 vials ea)

ELISA CEP01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Human Erythropoietin Quantikine IVD Control 3 (10 vials)

ELISA CEP03
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 4 reviews)

Have you used Human Erythropoietin Quantikine IVD ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human Erythropoietin Quantikine IVD ELISA Kit.

Filter by Ratings
Excellent
(4)
All Ratings
Filter by Applications
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
  Anonymous 04/02/2018
Show

Summary

Sample Tested mouse serum
  Excellent
  Anonymous 04/01/2018
Show

Summary

Sample Tested Serum
  Excellent
  Anonymous 12/01/2017
Show

Summary

Sample Tested A375 human melanoma cell line
 Human Erythropoietin Quantikine IVD ELISA Kit DEP00 Array DEP00
Enlarge
Excellent
  Anonymous 01/23/2017
Show
Image Details
 Human Erythropoietin Quantikine IVD ELISA Kit DEP00
: Human Erythropoietin Quantikine IVD ELISA Kit [DEP00].

Summary

Sample Tested EDTA Plasma

Other Experimental Details

Other Experimental Details For a series of measurement of EPO concentrations in human blood samples, we internally validated this assay. Our results were: inter-assay CV for 6 samples with differernt concentrations throughout the assay range 5.0 - 9.5 % (5 runs on 5 days), intra-Assay precision was 4.1%. 2 different kit lots were used. The Assay performs very well and reliable. Analysis of human blood samples was working well.

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee