Human Erythropoietin Quantikine IVD ELISA Kit

For In Vitro Diagnostic Use
(35 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol)
  • Sample Type & Volume Required Per Well
    Serum (100 uL), EDTA Plasma (100 uL)
  • Sensitivity
    0.6 mIU/mL
  • Assay Range
    2.5 - 200 mIU/mL (Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant human Epo
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with other substances.
Control Available
Product Summary
The Quantikine IVD Human Epo ELISA is designed to measure Epo levels in serum or plasma in less than 4.5 hours (or less than 2.5 hours using the shaker protocol) for the quantitative determination of erythropoietin (Epo) concentration in human serum and plasma as an aid in the diagnosis of anemia and polycythemia. For in vitro diagnostic use.

Intra-Assay Precision (Precision within an assay) Four control pools were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Four control pools were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0000000000000000


The recovery of human Epo spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (Benchtop Protocol) (n=10) 102
EDTA Plasma (Shaker Protocol) (n=10) 105
Serum (Benchtop Protocol) (n=10) 100
Serum (Shaker Protocol) (n=10) 93
To assess the linearity of the assay, samples spiked with high concentrations of Epo were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Erythropoietin Quantikine IVD ELISA Kit
Human Erythropoietin Quantikine IVD ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Erythropoietin
Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

    • Entrez Gene IDs
      2056 (Human); 13856 (Mouse); 24335 (Rat);
    • Alternate Names
      EP; EPO; epoetin; erythropoietin; MGC138142; MVCD2;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and specimens to room temperature before use. It is recommended that all specimens, standards, and controls be assayed in duplicate. Benchtop and shaker protocols are provided. The same protocol must be used throughout the assay.
    1.   Prepare all reagents as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Specimen
    6.   Add 100 µL of Standard, control, or specimen to each well. Cover with a plate sealer.
    7.   For Benchtop Protocol: Incubate at room temperature for 2 hours.
      For Shaker Protocol: Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
    8.   Thoroughly aspirate each well. Do not wash.

    9. 200 µL Conjugate
    10.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer.
    11.   For Benchtop Protocol: Incubate at room temperature for 2 hours.
      For Shaker Protocol: Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
    12.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    13. 200 µL Substrate Solution
    14. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20-25 minutes on the benchtop.

    15. 100 µL Stop Solution
    16. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 600 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 35
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    Sample Type
    1. Revisiting Biomarkers of Total-Body and Partial-Body Exposure in a Baboon Model of Irradiation.
      Authors: Valente M, Denis J, Grenier N, Arvers P, Foucher B, Desangles F, Martigne P, Chaussard H, Drouet M, Abend M, Herodin F
      PLoS ONE, 2015;10(7):e0132194.
      Species: Primate - Papio Anubis
      Sample Type: Plasma
    2. Sequence-engineered mRNA Without Chemical Nucleoside Modifications Enables an Effective Protein Therapy in Large Animals.
      Authors: Thess A, Grund S, Mui B, Hope M, Baumhof P, Fotin-Mleczek M, Schlake T
      Mol Ther, 2015;23(9):1456-64.
      Species: Primate - macaca fascicularis
      Sample Type: Serum
    3. Transgene regulation using the tetracycline-inducible TetR-KRAB system after AAV-mediated gene transfer in rodents and nonhuman primates.
      Authors: Le Guiner C, Stieger K, Toromanoff A, Guilbaud M, Mendes-Madeira A, Devaux M, Guigand L, Cherel Y, Moullier P, Rolling F, Adjali O
      PLoS ONE, 2014;9(9):e102538.
      Species: Macaca fascicularis
      Sample Type: Serum
    4. Mimicking hypoxia to treat anemia: HIF-stabilizer BAY 85-3934 (Molidustat) stimulates erythropoietin production without hypertensive effects.
      Authors: Flamme I, Oehme F, Ellinghaus P, Jeske M, Keldenich J, Thuss U
      PLoS ONE, 2014;9(11):e111838.
      Species: Primate - Cynomolgus Monkey
      Sample Type: Plasma
    5. Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease.
      Authors: Krautkramer E, Grouls S, Hettwer D, Rafat N, Tonshoff B, Zeier M
      J Virol, 2014;88(1):483-9.
      Species: Human
      Sample Type: Plasma
    6. Bone marrow mesenchymal stem cells in patients with beta thalassemia major: molecular analysis with attenuated total reflection-Fourier transform infrared spectroscopy study as a novel method.
      Stem Cells Dev., 2012;21(11):2000-11.
      Species: Human
      Sample Type: Bone Marrow Plasma
    7. Longevity of rAAV vector and plasmid DNA in blood after intramuscular injection in nonhuman primates: implications for gene doping.
      Authors: Ni W, Le Guiner C, Gernoux G
      Gene Ther., 2011;18(7):709-18.
      Species: Primate - Macaca fascicularis (Crab-eating or Cynomolgus macaque)
      Sample Type: Serum
    8. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
      Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
      Blood, 2011;117(13):3485-93.
      Species: Human
      Sample Type: Plasma
    9. Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
      Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
      J. Cardiothorac. Vasc. Anesth., 2009;23(3):316-23.
      Species: Human
      Sample Type: Plasma
    10. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
      Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
      Exp. Hematol., 2009;37(6):673-8.
      Species: Human
      Sample Type: Plasma
    11. Influence of inflammation as measured by alpha-1-acid glycoprotein on iron status indicators among HIV-positive postpartum Zimbabwean women.
      Authors: Rawat R, Stoltzfus RJ, Ntozini R, Mutasa K, Iliff PJ, Humphrey JH
      Eur J Clin Nutr, 2009;63(6):787-93.
      Species: Human
      Sample Type: Serum
    12. Alterations of systemic and muscle iron metabolism in human subjects treated with low-dose recombinant erythropoietin.
      Authors: Robach P, Recalcati S, Girelli D, Gelfi C, Aachmann-Andersen NJ, Thomsen JJ, Norgaard AM, Alberghini A, Campostrini N, Castagna A, Vigano A, Santambrogio P, Kempf T, Wollert KC, Moutereau S, Lundby C, Cairo G
      Blood, 2009;113(26):6707-15.
      Species: Human
      Sample Type: Plasma
    13. Pulmonary arterial hypertension and left-sided heart disease in sickle cell disease: clinical characteristics and association with soluble adhesion molecule expression.
      Authors: Klings ES, Anton Bland D, Rosenman D, Princeton S, Odhiambo A, Li G, Bernard SA, Steinberg MH, Farber HW
      Am. J. Hematol., 2008;83(7):547-53.
      Species: Human
      Sample Type: Plasma
    14. Iron metabolism in heterozygotes for hemoglobin E (HbE), alpha-thalassemia 1, or beta-thalassemia and in compound heterozygotes for HbE/beta-thalassemia.
      Authors: Zimmermann MB, Fucharoen S, Winichagoon P, Sirankapracha P, Zeder C, Gowachirapant S, Judprasong K, Tanno T, Miller JL, Hurrell RF
      Am. J. Clin. Nutr., 2008;88(4):1026-31.
      Species: Human
      Sample Type: Serum
    15. Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
      Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
      Species: Human
      Sample Type: Plasma
    16. Role of Gas6 in erythropoiesis and anemia in mice.
      Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
      J. Clin. Invest., 2008;118(2):583-96.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    17. Cerebrospinal fluid erythropoietin (EPO) in amyotrophic lateral sclerosis.
      Authors: Brettschneider J, Widl K, Schattauer D, Ludolph AC, Tumani H
      Neurosci. Lett., 2007;416(3):257-60.
      Species: Human
      Sample Type: Serum
    18. Strong iron demand during hypoxia-induced erythropoiesis is associated with down-regulation of iron-related proteins and myoglobin in human skeletal muscle.
      Authors: Robach P, Cairo G, Gelfi C, Bernuzzi F, Pilegaard H, Vigano A, Santambrogio P, Cerretelli P, Calbet JA, Moutereau S, Lundby C
      Blood, 2007;109(11):4724-31.
      Species: Human
      Sample Type: Serum
    19. Increased circulating endothelial progenitor cells in septic patients: correlation with survival.
      Authors: Rafat N, Hanusch C, Brinkkoetter PT, Schulte J, Brade J, Zijlstra JG, van der Woude FJ, van Ackern K, Yard BA, Beck GCh
      Crit. Care Med., 2007;35(7):1677-84.
      Species: Human
      Sample Type: Serum
    20. Oral administration of doxycycline allows tight control of transgene expression: a key step towards gene therapy of retinal diseases.
      Authors: Stieger K, Mendes-Madeira A, Meur GL, Weber M, Deschamps JY, Nivard D, Provost N, Moullier P, Rolling F
      Gene Ther., 2007;14(23):1668-73.
      Species: Primate - Macaca mulatta (Rhesus monkey)
      Sample Type: anterior chamber eye fluid
    21. Progress toward a nonviral gene therapy protocol for the treatment of anemia.
      Authors: Sebestyen MG, Hegge JO, Noble MA, Lewis DL, Herweijer H, Wolff JA
      Hum. Gene Ther., 2007;18(3):269-85.
      Species: Primate - Macaca mulatta (Rhesus monkey)
      Sample Type: Serum
    22. Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure.
      Authors: Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM
      J. Am. Coll. Cardiol., 2007;49(24):2341-9.
      Species: Human
      Sample Type: Serum
    23. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
      Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
      Am. J. Respir. Crit. Care Med., 2006;174(1):51-7.
      Species: Human
      Sample Type: Plasma
    24. Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
      Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
      Exp. Hematol., 2006;34(10):1312-22.
      Species: Human
      Sample Type: Serum
    25. Long-term doxycycline-regulated transgene expression in the retina of nonhuman primates following subretinal injection of recombinant AAV vectors.
      Authors: Stieger K, Le Meur G, Lasne F, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Martin L, Moullier P, Rolling F
      Mol. Ther., 2006;13(5):967-75.
      Species: Primate - Macaca mulatta (Rhesus monkey)
      Sample Type: Vitreous Humor
    26. Effects of chronic hypoxia in vivo on the expression of human placental glucose transporters.
      Authors: Zamudio S, Baumann MU, Illsley NP
      Placenta, 2006;27(1):49-55.
      Species: Human
      Sample Type: Serum
    27. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
      Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
      Br. J. Cancer, 2006;94(4):524-31.
      Species: Human
      Sample Type: Serum
    28. Haemoglobin E beta thalassaemia in Sri Lanka.
      Authors: Premawardhena A, Fisher CA, Olivieri NF, De Silva S, Arambepola M, Perera W, O&amp;amp;apos;Donnell A, O'Donnell A, O'Donnell A, O'Donnell A, O'Donnell A, Peto TE, Viprakasit V, Merson L, Muraca G, Weatherall DJ
      Lancet, 2005;366(9495):1467-70.
      Species: Human
      Sample Type: Serum
    29. Long-term pharmacologically regulated expression of erythropoietin in primates following AAV-mediated gene transfer.
      Authors: Rivera VM, Gao GP, Grant RL, Schnell MA, Zoltick PW, Rozamus LW, Clackson T, Wilson JM
      Blood, 2005;105(4):1424-30.
      Species: Primate - Macaca mulatta (Rhesus monkey)
      Sample Type: Serum
    30. Expansion of human SCID-repopulating cells under hypoxic conditions.
      Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
      J. Clin. Invest., 2003;112(1):126-35.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. Monoclonal myelopoiesis and subnormal erythropoietin concentration are independent risk factors for thromboembolic complications in essential thrombocythemia.
      Authors: Andreasson B, Harrison C, Lindstedt G, Linch D, Kutti J
      Blood, 2003;101(2):783.
      Species: Human
      Sample Type: Serum
    32. Erythropoietin therapy for acute stroke is both safe and beneficial.
      Authors: Ehrenreich H, Hasselblatt M, Dembowski C, Cepek L, Lewczuk P, Stiefel M, Rustenbeck HH, Breiter N, Jacob S, Knerlich F, Bohn M, Poser W, Ruther E, Kochen M, Gefeller O, Gleiter C, Wessel TC, De Ryck M, Itri L, Prange H, Cerami A, Brines M, Siren AL
      Mol. Med., 2002;8(8):495-505.
      Species: Human
      Sample Type: Serum
    33. Lack of an immune response against the tetracycline-dependent transactivator correlates with long-term doxycycline-regulated transgene expression in nonhuman primates after intramuscular injection of recombinant adeno-associated virus.
      Authors: Favre D, Blouin V, Provost N, Spisek R, Porrot F, Bohl D, Marme F, Cherel Y, Salvetti A, Hurtrel B, Heard JM, Riviere Y, Moullier P
      J. Virol., 2002;76(22):11605-11.
      Species: Primate - Macaca fascicularis (Crab-eating or Cynomolgus macaque)
      Sample Type: Serum
    34. Evaluation of tumor necrosis factor-alpha and erythropoietin serum levels in B-cell chronic lymphocytic leukemia patients with anemia.
      Authors: Capalbo S, Battista C, Delia M, Ciancio A, De Santis G, Dargenio M, Diomede D, Liso V
      Acta Haematol., 2002;108(2):84-9.
      Species: Human
      Sample Type: Serum
    35. Adeno-associated virus-mediated delivery of erythropoietin leads to sustained elevation of hematocrit in nonhuman primates.
      Authors: Zhou S, Murphy JE, Escobedo JA, Dwarki VJ
      Gene Ther., 1998;5(5):665-70.
      Species: Primate – Macaca fascicularis (Cynomolgus/Crab-eating Monkey)
      Sample Type: Serum
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    1. What type of EPO is used in the standard for the Human Erythropoietin Quantikine® IVD® ELISA kit (Catalog # DEP00)?

      • The standard in this kit contains CHO (Chinese Hamster Ovary) cell-derived Recombinant Human EPO.

    2. Can the Human Erythropoietin Quantikine® IVD® ELISA kit (Catalog # DEP00) be used with heparin plasma?

      • Heparin plasma samples are not validated nor recommended for use in the Human Erythropoietin Quantikine® IVD® ELISA kit. During kit validation, results of in-house testing did not meet our specifications.

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