Human Erythropoietin Quantikine IVD ELISA Kit DEP00: R&D Systems

Human Erythropoietin Quantikine IVD ELISA Kit

For In Vitro Diagnostic Use.
(49 citations)
(2 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol)
  • Sample Type & Volume Required Per Well
    Serum (100 uL), EDTA Plasma (100 uL)
  • Sensitivity
    0.6 mIU/mL
  • Assay Range
    2.5 - 200 mIU/mL (Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant human Epo
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with other substances.
Control Available
CEP01, Human Erythropoietin Quantikine IVD Control 1&2 (5 vials ea) - Please Inquire
CEP03, Human Erythropoietin Quantikine IVD Control 3 (10 vials) - Please Inquire
Product Summary
The Quantikine IVD Human Epo ELISA is designed to measure Epo levels in serum or plasma in less than 4.5 hours (or less than 2.5 hours using the shaker protocol) for the quantitative determination of erythropoietin (Epo) concentration in human serum and plasma as an aid in the diagnosis of anemia and polycythemia. For in vitro diagnostic use.

Precision
Intra-Assay Precision (Precision within an assay) Four control pools were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Four control pools were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample11223344
n3030303030303030
Mean5.826.4516.2216.0125.1826.3584.1479.02
Standard Deviation00000000
CV%4.957.823.022.845.222.182.423.06

Recovery

The recovery of human Epo spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (Benchtop Protocol) (n=10) 102
EDTA Plasma (Shaker Protocol) (n=10) 105
Serum (Benchtop Protocol) (n=10) 100
Serum (Shaker Protocol) (n=10) 93
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Epo were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Erythropoietin Quantikine IVD ELISA Kit
Human Erythropoietin Quantikine IVD ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Erythropoietin
Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

    • Entrez Gene IDs
      2056 (Human); 13856 (Mouse); 24335 (Rat);
    • Alternate Names
      EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and specimens to room temperature before use. It is recommended that all specimens, standards, and controls be assayed in duplicate. Benchtop and shaker protocols are provided. The same protocol must be used throughout the assay.
    1.   Prepare all reagents as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Specimen
    6.   Add 100 µL of Standard, control, or specimen to each well. Cover with a plate sealer.
    7.   For Benchtop Protocol: Incubate at room temperature for 2 hours.
      For Shaker Protocol: Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
    8.   Thoroughly aspirate each well. Do not wash.

    9. 200 µL Conjugate
    10.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer.
    11.   For Benchtop Protocol: Incubate at room temperature for 2 hours.
      For Shaker Protocol: Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
    12.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    13. 200 µL Substrate Solution
    14. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20-25 minutes on the benchtop.

    15. 100 µL Stop Solution
    16. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 600 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    49 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Anaemia in hospitalised preschool children from a rural area in Mozambique: a case control study in search for aetiological agents
      Authors: C Moraleda, R Aguilar, L Quintó, T Nhampossa, M Renom, A Nhabomba, S Acácio, JJ Aponte, D Nhalungo, AH Achtman, L Schofield, H Martins, E Macete, PL Alonso, C Menéndez
      BMC Pediatr, 2017;17(1):63.
      Species: Human
      Sample Type:
    2. Hemolytic anemia repressed hepcidin level without hepatocyte iron overload: lesson from G�nther disease model
      Authors: S Millot, C Delaby, B Moulouel, T Lefebvre, N Pilard, N Ducrot, C Ged, P Lett‚ron, L de Frances, JC Deybach, C Beaumont, L Gouya, H De Verneui, S Lyoumi, H Puy, Z Karim
      Haematologica, 2017;102(2):260-270.
      Species: Human
      Sample Type: Serum
    3. PlanHab: Hypoxia counteracts the erythropoietin suppression, but seems to exaggerate the plasma volume reduction induced by 3�weeks of bed rest
      Authors: ME Keramidas, IB Mekjavic, R Kölegård, A Choukèr, C Strewe, O Eiken
      Physiol Rep, 2016;4(7):.
      Species: Human
      Sample Type: Serum
    4. Diurnal changes of arterial oxygen saturation and erythropoietin concentration in male and female highlanders
      Physiol Rep, 2016;4(17):.
      Species: Human
      Sample Type: Plasma
    5. Erythropoietin Slows Photoreceptor Cell Death in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa
      Authors: Tonia S Rex
      PLoS ONE, 2016;11(6):e0157411.
      Species: Mouse
      Sample Type: Serum
    6. Improved Human Erythropoiesis and Platelet Formation in Humanized NSGW41 Mice
      Stem Cell Reports, 2016;0(0):.
      Species: Human
      Sample Type: Serum
    7. Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system
      Gene Ther., 2016;23(10):699-707.
      Species: Human
      Sample Type: Serum
    8. Sequence-engineered mRNA Without Chemical Nucleoside Modifications Enables an Effective Protein Therapy in Large Animals.
      Authors: Thess A, Grund S, Mui B, Hope M, Baumhof P, Fotin-Mleczek M, Schlake T
      Mol Ther, 2015;23(9):1456-64.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Serum
    9. Revisiting Biomarkers of Total-Body and Partial-Body Exposure in a Baboon Model of Irradiation.
      Authors: Valente M, Denis J, Grenier N, Arvers P, Foucher B, Desangles F, Martigne P, Chaussard H, Drouet M, Abend M, Herodin F
      PLoS ONE, 2015;10(7):e0132194.
      Species: Primate - Papio anubis (Olive Baboon)
      Sample Type: Plasma
    10. Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
      Authors: de Lucas Cerrillo A, Bond W, Rex T
      Gene Ther, 2015;22(5):365-73.
      Species: Hamster
      Sample Type: Cell Culture Supernates
    11. Transgene regulation using the tetracycline-inducible TetR-KRAB system after AAV-mediated gene transfer in rodents and nonhuman primates.
      Authors: Le Guiner C, Stieger K, Toromanoff A, Guilbaud M, Mendes-Madeira A, Devaux M, Guigand L, Cherel Y, Moullier P, Rolling F, Adjali O
      PLoS ONE, 2014;9(9):e102538.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Serum
    12. Hypoxic response contributes to altered gene expression and precapillary pulmonary hypertension in patients with sickle cell disease.
      Authors: Zhang X, Zhang W, Ma S, Desai A, Saraf S, Miasniakova G, Sergueeva A, Ammosova T, Xu M, Nekhai S, Abbasi T, Casanova N, Steinberg M, Baldwin C, Sebastiani P, Prchal J, Kittles R, Garcia J, Machado R, Gordeuk V
      Circulation, 2014;129(16):1650-8.
      Species: Human
      Sample Type: Serum
    13. Mimicking hypoxia to treat anemia: HIF-stabilizer BAY 85-3934 (Molidustat) stimulates erythropoietin production without hypertensive effects.
      Authors: Flamme I, Oehme F, Ellinghaus P, Jeske M, Keldenich J, Thuss U
      PLoS ONE, 2014;9(11):e111838.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Plasma
    14. Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease.
      Authors: Krautkramer E, Grouls S, Hettwer D, Rafat N, Tonshoff B, Zeier M
      J Virol, 2014;88(1):483-9.
      Species: Human
      Sample Type: Plasma
    15. Erythropoietin rs1617640 G allele associates with an attenuated rise of serum erythropoietin and a marked decline of hemoglobin in hepatitis C patients undergoing antiviral therapy.
      Authors: Amanzada A, Goralczyk A, Reinhardt L, Moriconi F, Cameron S, Mihm S
      BMC Infect Dis, 2014;14(0):503.
      Species: Human
      Sample Type: Serum
    16. The relation of hepcidin to iron disorders, inflammation and hemoglobin in chronic kidney disease.
      Authors: Mercadel L, Metzger M, Haymann J, Thervet E, Boffa J, Flamant M, Vrtovsnik F, Houillier P, Froissart M, Stengel B
      PLoS ONE, 2014;9(6):e99781.
      Species: Human
      Sample Type: Serum
    17. Targeted delivery of erythropoietin by transcranial focused ultrasound for neuroprotection against ischemia/reperfusion-induced neuronal injury: a long-term and short-term study.
      Authors: Wu, Sheng-Ka, Yang, Ming-Tao, Kang, Kai-Hsia, Liou, Houng-Ch, Lu, Dai-Hua, Fu, Wen-Mei, Lin, Win-Li
      PLoS ONE, 2014;9(2):e90107.
      Species: Rat
      Sample Type: Tissue Homogenates
    18. The effect of the neonatal Fc receptor on human IgG biodistribution in mice.
      Authors: Chen, Nancy, Wang, Weirong, Fauty, Scott, Fang, Yulin, Hamuro, Lora, Hussain, Azher, Prueksaritanont, Thomayan
      MAbs, 2014;6(2):502-8.
      Species: Mouse
      Sample Type: Plasma
    19. Hematological parameters, and hematopoietic growth factors: EPO and IL-3 in response to whole-body cryostimulation (WBC) in military academy students.
      Authors: Szygula Z, Lubkowska A, Giemza C, Skrzek A, Bryczkowska I, Dolegowska B
      PLoS ONE, 2014;9(4):e93096.
      Species: Human
      Sample Type: Plasma
    20. Bone marrow mesenchymal stem cells in patients with beta thalassemia major: molecular analysis with attenuated total reflection-Fourier transform infrared spectroscopy study as a novel method.
      Stem Cells Dev., 2012;21(11):2000-11.
      Species: Human
      Sample Type: Bone Marrow Plasma
    21. Longevity of rAAV vector and plasmid DNA in blood after intramuscular injection in nonhuman primates: implications for gene doping.
      Authors: Ni W, Le Guiner C, Gernoux G
      Gene Ther., 2011;18(7):709-18.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Serum
    22. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
      Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
      Blood, 2011;117(13):3485-93.
      Species: Human
      Sample Type: Plasma
    23. Alterations of systemic and muscle iron metabolism in human subjects treated with low-dose recombinant erythropoietin.
      Authors: Robach P, Recalcati S, Girelli D, Gelfi C, Aachmann-Andersen NJ, Thomsen JJ, Norgaard AM, Alberghini A, Campostrini N, Castagna A, Vigano A, Santambrogio P, Kempf T, Wollert KC, Moutereau S, Lundby C, Cairo G
      Blood, 2009;113(26):6707-15.
      Species: Human
      Sample Type: Plasma
    24. Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
      Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
      J. Cardiothorac. Vasc. Anesth., 2009;23(3):316-23.
      Species: Human
      Sample Type: Plasma
    25. Influence of inflammation as measured by alpha-1-acid glycoprotein on iron status indicators among HIV-positive postpartum Zimbabwean women.
      Authors: Rawat R, Stoltzfus RJ, Ntozini R, Mutasa K, Iliff PJ, Humphrey JH
      Eur J Clin Nutr, 2009;63(6):787-93.
      Species: Human
      Sample Type: Serum
    26. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
      Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
      Exp. Hematol., 2009;37(6):673-8.
      Species: Human
      Sample Type: Plasma
    27. Role of Gas6 in erythropoiesis and anemia in mice.
      Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
      J. Clin. Invest., 2008;118(2):583-96.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    28. Iron metabolism in heterozygotes for hemoglobin E (HbE), alpha-thalassemia 1, or beta-thalassemia and in compound heterozygotes for HbE/beta-thalassemia.
      Authors: Zimmermann MB, Fucharoen S, Winichagoon P, Sirankapracha P, Zeder C, Gowachirapant S, Judprasong K, Tanno T, Miller JL, Hurrell RF
      Am. J. Clin. Nutr., 2008;88(4):1026-31.
      Species: Human
      Sample Type: Serum
    29. Pulmonary arterial hypertension and left-sided heart disease in sickle cell disease: clinical characteristics and association with soluble adhesion molecule expression.
      Authors: Klings ES, Anton Bland D, Rosenman D, Princeton S, Odhiambo A, Li G, Bernard SA, Steinberg MH, Farber HW
      Am. J. Hematol., 2008;83(7):547-53.
      Species: Human
      Sample Type: Plasma
    30. Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
      Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
      2008;84(0):481.
      Species: Human
      Sample Type: Plasma
    31. Progress toward a nonviral gene therapy protocol for the treatment of anemia.
      Authors: Sebestyen MG, Hegge JO, Noble MA, Lewis DL, Herweijer H, Wolff JA
      Hum. Gene Ther., 2007;18(3):269-85.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Serum
    32. Oral administration of doxycycline allows tight control of transgene expression: a key step towards gene therapy of retinal diseases.
      Authors: Stieger K, Mendes-Madeira A, Meur GL, Weber M, Deschamps JY, Nivard D, Provost N, Moullier P, Rolling F
      Gene Ther., 2007;14(23):1668-73.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Aqueous humor
    33. Strong iron demand during hypoxia-induced erythropoiesis is associated with down-regulation of iron-related proteins and myoglobin in human skeletal muscle.
      Authors: Robach P, Cairo G, Gelfi C, Bernuzzi F, Pilegaard H, Vigano A, Santambrogio P, Cerretelli P, Calbet JA, Moutereau S, Lundby C
      Blood, 2007;109(11):4724-31.
      Species: Human
      Sample Type: Serum
    34. Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure.
      Authors: Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM
      J. Am. Coll. Cardiol., 2007;49(24):2341-9.
      Species: Human
      Sample Type: Serum
    35. Cerebrospinal fluid erythropoietin (EPO) in amyotrophic lateral sclerosis.
      Authors: Brettschneider J, Widl K, Schattauer D, Ludolph AC, Tumani H
      Neurosci. Lett., 2007;416(3):257-60.
      Species: Human
      Sample Type: Serum
    36. Increased circulating endothelial progenitor cells in septic patients: correlation with survival.
      Authors: Rafat N, Hanusch C, Brinkkoetter PT, Schulte J, Brade J, Zijlstra JG, van der Woude FJ, van Ackern K, Yard BA, Beck GCh
      Crit. Care Med., 2007;35(7):1677-84.
      Species: Human
      Sample Type: Serum
    37. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
      Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
      Br. J. Cancer, 2006;94(4):524-31.
      Species: Human
      Sample Type: Serum
    38. Long-term doxycycline-regulated transgene expression in the retina of nonhuman primates following subretinal injection of recombinant AAV vectors.
      Authors: Stieger K, Le Meur G, Lasne F, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Martin L, Moullier P, Rolling F
      Mol. Ther., 2006;13(5):967-75.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Vitreous Humor
    39. Effects of chronic hypoxia in vivo on the expression of human placental glucose transporters.
      Authors: Zamudio S, Baumann MU, Illsley NP
      Placenta, 2006;27(1):49-55.
      Species: Human
      Sample Type: Serum
    40. Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
      Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
      Exp. Hematol., 2006;34(10):1312-22.
      Species: Human
      Sample Type: Serum
    41. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
      Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
      Am. J. Respir. Crit. Care Med., 2006;174(1):51-7.
      Species: Human
      Sample Type: Plasma
    42. Long-term pharmacologically regulated expression of erythropoietin in primates following AAV-mediated gene transfer.
      Authors: Rivera VM, Gao GP, Grant RL, Schnell MA, Zoltick PW, Rozamus LW, Clackson T, Wilson JM
      Blood, 2005;105(4):1424-30.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Serum
    43. Haemoglobin E beta thalassaemia in Sri Lanka.
      Authors: Premawardhena A, Fisher CA, Olivieri NF, De Silva S, Arambepola M, Perera W, O&amp;amp;apos;Donnell A, O'Donnell A, O'Donnell A, O'Donnell A, O'Donnell A, Peto TE, Viprakasit V, Merson L, Muraca G, Weatherall DJ
      Lancet, 2005;366(9495):1467-70.
      Species: Human
      Sample Type: Serum
    44. Expansion of human SCID-repopulating cells under hypoxic conditions.
      Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
      J. Clin. Invest., 2003;112(1):126-35.
      Species: Human
      Sample Type: Cell Culture Supernates
    45. Monoclonal myelopoiesis and subnormal erythropoietin concentration are independent risk factors for thromboembolic complications in essential thrombocythemia.
      Authors: Andreasson B, Harrison C, Lindstedt G, Linch D, Kutti J
      Blood, 2003;101(2):783.
      Species: Human
      Sample Type: Serum
    46. Evaluation of tumor necrosis factor-alpha and erythropoietin serum levels in B-cell chronic lymphocytic leukemia patients with anemia.
      Authors: Capalbo S, Battista C, Delia M, Ciancio A, De Santis G, Dargenio M, Diomede D, Liso V
      Acta Haematol., 2002;108(2):84-9.
      Species: Human
      Sample Type: Serum
    47. Erythropoietin therapy for acute stroke is both safe and beneficial.
      Authors: Ehrenreich H, Hasselblatt M, Dembowski C, Cepek L, Lewczuk P, Stiefel M, Rustenbeck HH, Breiter N, Jacob S, Knerlich F, Bohn M, Poser W, Ruther E, Kochen M, Gefeller O, Gleiter C, Wessel TC, De Ryck M, Itri L, Prange H, Cerami A, Brines M, Siren AL
      Mol. Med., 2002;8(8):495-505.
      Species: Human
      Sample Type: Serum
    48. Lack of an immune response against the tetracycline-dependent transactivator correlates with long-term doxycycline-regulated transgene expression in nonhuman primates after intramuscular injection of recombinant adeno-associated virus.
      Authors: Favre D, Blouin V, Provost N, Spisek R, Porrot F, Bohl D, Marme F, Cherel Y, Salvetti A, Hurtrel B, Heard JM, Riviere Y, Moullier P
      J. Virol., 2002;76(22):11605-11.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Serum
    49. Adeno-associated virus-mediated delivery of erythropoietin leads to sustained elevation of hematocrit in nonhuman primates.
      Authors: Zhou S, Murphy JE, Escobedo JA, Dwarki VJ
      Gene Ther., 1998;5(5):665-70.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Serum
    Expand to show all 49 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Human Erythropoietin Quantikine IVD Control 1&2 (5 vials ea)

    ELISA CEP01
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    Human Erythropoietin Quantikine IVD Control 3 (10 vials)

    ELISA CEP03
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    Sample TestedA375 human melanoma cell line
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     Human Erythropoietin Quantikine IVD ELISA Kit DEP00
    : Human Erythropoietin Quantikine IVD ELISA Kit [DEP00]

    Summary

    Sample TestedEDTA Plasma

    Other Experimental Details

    Other Experimental DetailsFor a series of measurement of EPO concentrations in human blood samples, we internally validated this assay. Our results were: inter-assay CV for 6 samples with differernt concentrations throughout the assay range 5.0 - 9.5 % (5 runs on 5 days), intra-Assay precision was 4.1%. 2 different kit lots were used. The Assay performs very well and reliable. Analysis of human blood samples was working well.

    FAQs

    1. What type of EPO is used in the standard for the Human Erythropoietin Quantikine® IVD® ELISA kit (Catalog # DEP00)?

      • The standard in this kit contains CHO (Chinese Hamster Ovary) cell-derived Recombinant Human EPO.

    2. Can the Human Erythropoietin Quantikine® IVD® ELISA kit (Catalog # DEP00) be used with heparin plasma?

      • Heparin plasma samples are not validated nor recommended for use in the Human Erythropoietin Quantikine® IVD® ELISA kit. During kit validation, results of in-house testing did not meet our specifications.

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