Human ETV6 Antibody

ETV6 (ETS Translocation Variant 6; also TEL [Translocated ETS Leukemia]) is a 58-62 kDa member of the ETS (E-Twenty Six avian leukemia virus) family of proteins. It belongs to a particular class of repressors that are controlled by cell-cycle dependent signals. ETV6 is ubiquitously expressed, and acts as a sequence-specific transcriptional repressor of ETS-binding site-containing gene promoters. It likely does this by recruiting histone deacetylase to its binding site. It is also involved in steroid signaling, and is reported to bind to the DNA binding domains of both RAR and RXR. ETV6 appears to be important in coordinating bone marrow-mediated hematopoiesis. Genes known to be responsive to ETV6 regulation include FLI1, IDI and MMP-3. Human ETV6 is 452 amino acids (aa) in length. It contains one PNT (pointed)/B domain that mediates oligomerization (aa 40-124) followed by an ETS domain that binds DNA (aa 338-422). There are five known utilized Thr/Ser phosphorylation sites plus at least one alternative start site at Met43. This short form is very effective at gene repression due to the absence of a SUMOylation site at Lys11 that, when occupied, blocks DNA binding. Although ETV6 activity is dependent upon homooligomerization, it is also known to bind to TEL2 and FLI1. ETS genes are often involved in chromosomal translocations. ETV6 itself is associated with multiple rearrangements, including the genes encoding AML1, PDGFR beta and BTL (Brx-like Translocated in Leukemia). The translocated sequences of ETV6 differ from chimera to chimera. For example, aa 1-336 are fused to a portion of AML1, aa 1-154 are fused to a portion of PDGFR beta, and aa 12-433 are fused to a portion of BTL. Over aa 2-111, human ETV6 shares 91% aa sequence identity with mouse ETV6.