Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all F-box protein 15/FBXO15 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human F‑box protein 15/FBXO15
aa 298-434
Accession # NP_689889

Specificity

Detects human F‑box protein 15/FBXO15 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse F‑box protein 15/FBXO15 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human F‑box protein 15/FBXO15 Antibody

Detection of Human F-box protein 15/FBXO15 antibody by Western Blot.

Detection of Human F-box protein 15/FBXO15 by Western Blot.

Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human F-box protein 15/FBXO15 Antibody Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6035) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for F-box protein 15/FBXO15 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human F‑box protein 15/FBXO15 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: F-box protein 15/FBXO15

FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site, and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15.

Alternate Names

Fbox protein 15, FBX15, FBXO15

Entrez Gene IDs

201456 (Human); 361354 (Rat)

Gene Symbol

FBXO15

UniProt

NP_689889

Additional F-box protein 15/FBXO15 Products

Product Documents for Human F‑box protein 15/FBXO15 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human F‑box protein 15/FBXO15 Antibody

For research use only

Related Research Areas

E3 Ubiquitin Ligases

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