Detection of Human F‑box protein 15/FBXO15 by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human F‑box protein 15/FBXO15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6035) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for F‑box protein 15/FBXO15 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: F-box protein 15/FBXO15
F-box protein 15 (FBXO15) is a member of the F-box family “other” category. These proteins contribute to substrate specificity of cullin-based E3 ubiquitin ligase complexes. FBXO15 is a target of the Oct3/4 transcription factor in undifferentiated embryonic stem cells and germline cells.
Entrez Gene IDs:
201456 (Human); 361354 (Rat)
F-box only protein 15; Fbox protein 15; F-box protein 15; FBX15; FBX15MGC39671; FBXO15
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