Fatty acid binding protein-2 (FABP2; also I- or intestinal FABP) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1‑3). FABP2 is one of nine cytoplasmic FABPs that are 14‑15 kDa in size and range from 126‑134 amino acids (aa) in length (2). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Nevertheless, based on aa sequence, the nine FABP family members have been shown to form three subgroups, with FABP2/I-FABP linked with liver/L-FABP and heart/H-FABP (2). The designation of a tissue type, such as intestinal, does not suggest the binding protein is universally expressed in all cell types that make up the organ or tissue. Human I-FABP, the product of the FABP-2 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure (called a beta -clam) generated by a series of antiparallel beta -strands and two alpha -helices (1, 2, 4). Preferred ligands for FABP2 include sixteen to twenty carbon long chain fatty acids (4). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in “internalization” of the ligand.(1) An Ala-to-Thr polymorphism at position # 54 has been reported to potentially impact FABP2 function (2). This polymorphism has been suggested to be associated with an increased risk of type II diabetes. To date, the evidence appears to be equivocal (1, 2). This polymorphism may, however, have unusual metabolic effects depending upon the type of diet involved (1, 5). Human FABP-2 is 78%, 82% and 86% aa identical to mouse, rat and canine FABP2, respectively. It also shows 33% and 24% aa identity to human H-FABP and L‑FABP, respectively. FABP2 is proposed to transport fatty acids (FA) into cells, increase FA availability to enzymes, protect cell structures from FA attack, and target FA to transcription factors in the nuclear lumen (3).
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human FABP2/I‑FABP
Ala2-Asp132
Accession # P12104
Ala2-Asp132
Accession # P12104
Specificity
Detects human FABP2/I‑FABP in ELISAs and Western blots. In sandwich immunoassays, less than 0.4% cross-reactivity with recombinant rat FABP2 is observed, and less than 0.1% cross-reactivity with recombinant human (rh) FABP1, -3, -5, -6, -7, -8, rhFABP9, recombinant mouse (rm) FABP4, and rmFABP9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human FABP2/I‑FABP Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human FABP2/I‑FABP (Catalog # 2694-CL/CF)
Sample: Recombinant Human FABP2/I‑FABP (Catalog # 2694-CL/CF)
Human FABP2 Sandwich Immunoassay
ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
Use in combination with these reagents:
- Capture Reagent: Human/Mouse FABP2/I‑FABP Antibody (Catalog # MAB30781)
- Standard: Recombinant Human FABP2/I-FABP Protein, CF (Catalog # 2694-CL/CF)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using BAF3078 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FABP2/I-FABP
References
- Weiss, E.P. et al. (2002) Physiol. Genomics 10:145.
- Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
- Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
- Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
- Dworatzek, P. et al. (2004) Am. J. Clin. Nutr. 79:1110.
Long Name
Fatty Acid-Binding Protein 2/Intestinal FABP
Alternate Names
I-FABP, IFABP, Intestinal FABP
Gene Symbol
FABP2
UniProt
Additional FABP2/I-FABP Products
Product Documents for Human FABP2/I‑FABP Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FABP2/I‑FABP Biotinylated Antibody
For research use only
Related Research Areas
Customer Reviews for Human FABP2/I‑FABP Biotinylated Antibody (2)
4.5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: Human cellsSpecies: MouseVerified Customer | Posted 08/21/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Troubleshooting Guide
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