Human FABP2/I-FABP Antibody [Biotin]

R&D Systems | Catalog # BAF3078

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all FABP2/I-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP2/I‑FABP
Ala2-Asp132
Accession # P12104

Specificity

Detects human FABP2/I‑FABP in ELISAs and Western blots. In sandwich immunoassays, less than 0.4% cross-reactivity with recombinant rat FABP2 is observed, and less than 0.1% cross-reactivity with recombinant human (rh) FABP1, -3, -5, -6, -7, -8, rhFABP9, recombinant mouse (rm) FABP4, and rmFABP9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FABP2/I-FABP Antibody [Biotin]

Human FABP2 / I-FABP ELISA Standard Curve

Human FABP2 / I-FABP ELISA Standard Curve

Recombinant Human FABP2/I‑FABP (Catalog # 2694-CL/CF) was serially diluted and captured by Mouse Anti-Human/Mouse FABP2/I‑FABP Monoclonal Antibody (Catalog # MAB30781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FABP2/I‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3078) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human FABP2/I-FABP Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human FABP2/I‑FABP (Catalog # 2694-CL/CF)

Human FABP2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Mouse FABP2/I‑FABP Antibody (Catalog # MAB30781)
  • Standard: Recombinant Human FABP2/I-FABP Protein, CF (Catalog # 2694-CL/CF)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF3078 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP2/I-FABP

Fatty acid binding protein-2 (FABP2; also I- or intestinal FABP) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1‑3). FABP2 is one of nine cytoplasmic FABPs that are 14‑15 kDa in size and range from 126‑134 amino acids (aa) in length (2). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Nevertheless, based on aa sequence, the nine FABP family members have been shown to form three subgroups, with FABP2/I-FABP linked with liver/L-FABP and heart/H-FABP (2). The designation of a tissue type, such as intestinal, does not suggest the binding protein is universally expressed in all cell types that make up the organ or tissue. Human I-FABP, the product of the FABP-2 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure (called a beta -clam) generated by a series of antiparallel beta -strands and two alpha -helices (1, 2, 4). Preferred ligands for FABP2 include sixteen to twenty carbon long chain fatty acids (4). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in “internalization” of the ligand.(1) An Ala-to-Thr polymorphism at position # 54 has been reported to potentially impact FABP2 function (2). This polymorphism has been suggested to be associated with an increased risk of type II diabetes. To date, the evidence appears to be equivocal (1, 2). This polymorphism may, however, have unusual metabolic effects depending upon the type of diet involved (1, 5). Human FABP-2 is 78%, 82% and 86% aa identical to mouse, rat and canine FABP2, respectively. It also shows 33% and 24% aa identity to human H-FABP and L‑FABP, respectively. FABP2 is proposed to transport fatty acids (FA) into cells, increase FA availability to enzymes, protect cell structures from FA attack, and target FA to transcription factors in the nuclear lumen (3).

References

  1. Weiss, E.P. et al. (2002) Physiol. Genomics 10:145.
  2. Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
  3. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
  4. Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
  5. Dworatzek, P. et al. (2004) Am. J. Clin. Nutr. 79:1110.

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Alternate Names

I-FABP, IFABP, Intestinal FABP

Entrez Gene IDs

2169 (Human); 25598 (Rat)

Gene Symbol

FABP2

UniProt

P12104

Additional FABP2/I-FABP Products

Product Documents for Human FABP2/I-FABP Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Human FABP2/I-FABP Antibody [Biotin]

For research use only

Related Research Areas

Early Endodermal Lineage Markers
Obesity

Customer Reviews for Human FABP2/I-FABP Antibody [Biotin] (2)

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  • Human FABP2/I-FABP Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human cells
    Species: Mouse
    Verified Customer | Posted 08/21/2018
  • Human FABP2/I-FABP Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 12/05/2017

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