FABP2/I-FABP Antibody (323730)

R&D Systems | Catalog # MAB30781

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 323730
View all FABP2/I-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP2/I‑FABP
Ala2-Asp132
Accession # P12104

Specificity

Detects human FABP2/I‑FABP in ELISAs. Detects human and mouse FABP2/I‑FABP in Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human FABP1, 3, 5, 6, 7, 8, 9, recombinant mouse FABP4, 9, or recombinant rat FABP2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FABP2/I-FABP Antibody (323730)

Detection of Human and Mouse FABP2/I-FABP antibody by Western Blot.

Detection of Human and Mouse FABP2/I‑FABP by Western Blot.

Western blot shows lysates of human small intestine tissue and mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FABP2/I-FABP Monoclonal Antibody (Catalog # MAB30781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP2/I-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP2/I-FABP antibody in Human Small Intestine by Immunohistochemistry (IHC-P).

FABP2/I‑FABP in Human Small Intestine.

FABP2/I-FABP was detected in perfusion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human/Mouse FABP2/I-FABP Monoclonal Antibody (Catalog # MAB30781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Human FABP2 / I-FABP ELISA Standard Curve

Human FABP2 / I-FABP ELISA Standard Curve

Recombinant Human FABP2/I‑FABP (Catalog # 2694-CL/CF) was serially diluted and captured by Mouse Anti-Human/Mouse FABP2/I‑FABP Monoclonal Antibody (Catalog # MAB30781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FABP2/I‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3078) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for FABP2/I-FABP Antibody (323730)

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample:

Perfusion fixed paraffin-embedded sections of human small intestine

Western Blot

2 µg/mL
Sample: Human small intestine tissue and mouse small intestine tissue

Human FABP2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human FABP2/I‑FABP Biotinylated Antibody (Catalog # BAF3078)
  • Standard: Recombinant Human FABP2/I-FABP Protein, CF (Catalog # 2694-CL/CF)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB30781 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: FABP2/I-FABP

Fatty acid binding protein-2 (FABP2; also I- or intestinal FABP) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1‑3). FABP2 is one of nine cytoplasmic FABPs that are 14‑15 kDa in size and range from 126‑134 amino acids (aa) in length (2). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Nevertheless, based on aa sequence, the nine FABP family members have been shown to form three subgroups, with FABP2/I-FABP linked with liver/L-FABP and heart/H-FABP (2). The designation of a tissue type, such as intestinal, does not suggest the binding protein is universally expressed in all cell types that make up the organ or tissue. Human I-FABP, the product of the FABP-2 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure (called a beta -clam) generated by a series of antiparallel beta -strands and two alpha -helices (1, 2, 4). Preferred ligands for FABP2 include sixteen to twenty carbon long chain fatty acids (4). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in “internalization” of the ligand.(1) An Ala-to-Thr polymorphism at position # 54 has been reported to potentially impact FABP2 function (2). This polymorphism has been suggested to be associated with an increased risk of type II diabetes. To date, the evidence appears to be equivocal (1, 2). This polymorphism may, however, have unusual metabolic effects depending upon the type of diet involved (1, 5). Human FABP-2 is 78%, 82% and 86% aa identical to mouse, rat and canine FABP2, respectively. It also shows 33% and 24% aa identity to human H-FABP and L‑FABP, respectively. FABP2 is proposed to transport fatty acids (FA) into cells, increase FA availability to enzymes, protect cell structures from FA attack, and target FA to transcription factors in the nuclear lumen (3).

References

  1. Weiss, E.P. et al. (2002) Physiol. Genomics 10:145.
  2. Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
  3. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
  4. Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
  5. Dworatzek, P. et al. (2004) Am. J. Clin. Nutr. 79:1110.

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Alternate Names

I-FABP, IFABP, Intestinal FABP

Entrez Gene IDs

2169 (Human); 25598 (Rat)

Gene Symbol

FABP2

UniProt

P12104

Additional FABP2/I-FABP Products

Product Documents for FABP2/I-FABP Antibody (323730)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Select SDS Language

Can't find your language? Request a SDS in your language here.

Product Specific Notices for FABP2/I-FABP Antibody (323730)

For research use only

Related Research Areas

Early Endodermal Lineage Markers
Obesity

Citations for FABP2/I-FABP Antibody (323730)

Customer Reviews for FABP2/I-FABP Antibody (323730) (4)

4.5 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used FABP2/I-FABP Antibody (323730)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human/Mouse FABP2/I-FABP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Intestine
    Species: Mouse
    Verified Customer | Posted 06/16/2022
    FABP2/I-FABP Antibody (323730) MAB30781
  • Human/Mouse FABP2/I-FABP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Mesothelioma
    Species: Human
    Verified Customer | Posted 12/15/2021
    FABP2/I-FABP Antibody (323730) MAB30781
  • Human/Mouse FABP2/I-FABP Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human cells
    Species: Mouse
    Verified Customer | Posted 08/21/2018
  • Human/Mouse FABP2/I-FABP Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 12/05/2017

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies