Human FCRLB/FCRY Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FCRLB/FCRY in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) FCRLB/FCRY is observed, and less than 1% cross-reactivity with recombinant human FCRN and rmFCRN is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FCRLB/FCRY
    Ala18-Ser426
    Accession # Q6BAA4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FCRLB/FCRY by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human FCRLB/FCRY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4705) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FCRLB/FCRY at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 57-60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified human IgG with high affinity. Human FCRLB shares 82% and 85% amino acid sequence identity with mouse and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1 - 3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1 - 3). Alternative splicing generates isoforms with deletions in the putative signal peptide and substitutions and/or truncations in the third Ig-like domain (3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (3, 4). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (3, 4). It is upregulated in B cells by BAFF and LPS (5). Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 5). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (4, 5).

  • References:
    1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
    2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
    3. Chikaev, N.A. et al. (2005) Genomics 85:264.
    4. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
    5. Masuda, K. et al. (2005) Gene 363:32.
  • Long Name:
    Fc Receptor-like B
  • Entrez Gene IDs:
    127943 (Human); 435653 (Mouse); 498275 (Rat)
  • Alternate Names:
    Fc receptor homolog expressed in B cells protein 2; Fc receptor like 2; Fc receptor-like and mucin-like 2; Fc receptor-like and mucin-like protein 2; Fc receptor-like B; Fc receptor-like protein 2; Fc receptor-related protein Y; FcRL2; FCRL2FCRLM2; FCRLB; FCRLM2; FCRLY; FCRY; FLJ31052; FREB2; FREB-2; FREB-2FcRY; RP11-474I16.6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

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Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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