Human FCRLB/FCRY Antibody

Catalog # Availability Size / Price Qty
AF4705
AF4705-SP
Detection of Human FCRLB/FCRY by Western Blot.
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Human FCRLB/FCRY Antibody Summary

Species Reactivity
Human
Specificity
Detects human FCRLB/FCRY in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) FCRLB/FCRY is observed, and less than 1% cross-reactivity with recombinant human FCRN and rmFCRN is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FCRLB/FCRY
Ala18-Ser426
Accession # Q6BAA4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human FCRLB/FCRY by Western Blot. View Larger

Detection of Human FCRLB/FCRY by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human FCRLB/FCRY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4705) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FCRLB/FCRY at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 57-60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified human IgG with high affinity. Human FCRLB shares 82% and 85% amino acid sequence identity with mouse and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1 - 3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1 - 3). Alternative splicing generates isoforms with deletions in the putative signal peptide and substitutions and/or truncations in the third Ig-like domain (3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (3, 4). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (3, 4). It is upregulated in B cells by BAFF and LPS (5). Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 5). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (4, 5).

References
  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Chikaev, N.A. et al. (2005) Genomics 85:264.
  4. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
  5. Masuda, K. et al. (2005) Gene 363:32.
Long Name
Fc Receptor-like B
Entrez Gene IDs
127943 (Human); 435653 (Mouse); 498275 (Rat)
Alternate Names
Fc receptor homolog expressed in B cells protein 2; Fc receptor like 2; Fc receptor-like and mucin-like 2; Fc receptor-like and mucin-like protein 2; Fc receptor-like B; Fc receptor-like protein 2; Fc receptor-related protein Y; FcRL2; FCRL2FCRLM2; FCRLB; FCRLM2; FCRLY; FCRY; FLJ31052; FREB2; FREB-2; FREB-2FcRY; RP11-474I16.6

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