Human FERMT3 Antibody

Catalog # Availability Size / Price Qty
AF7004
AF7004-SP
Detection of Human FERMT3 by Western Blot.
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Human FERMT3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FERMT3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) FERMT2 is observed and less than 1% cross-reactivity with rhFERMT1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FERMT3
Ala498-Phe667
Accession # Q86UX7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human FERMT3 by Western Blot. View Larger

Detection of Human FERMT3 by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human FERMT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7004) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FERMT3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FERMT3

 

FERMT3 (Fermitin family homolog 3; also kindling-3, MIG2B and URP2) is a 72-78 kDa member of the kindling family of proteins. It is a cytoplasmic protein that is expressed by B cells, T cells, platelets, neutrophils and vascular endothelial cells. FERMT3 acts on the cytoplasmic tails of beta -integrins 1 thru 3 to induce a conformational change and activation. This allows for expressing cell adhesion and arrest. Human FERMT3 is 667 amino acids (aa) in length. It contains a poly-Lys region (aa 147-155) plus a FERM domain (aa 229-558) that itself possesses a membrane-anchoring Pleckstrin homology domain (aa 354-457). There are three utilized phosphorylation sites at Ser8, Tyr11 and Tyr504. There are also two splice variants. One is 56 kDa in size and represents the use of an alternative start site at Met181, while a second contains a deletion of aa 360-363. Over aa 498-667, human FERMT3 shares 99% aa identity with mouse FERMT3.

Long Name
Fermitin Family Member 3
Entrez Gene IDs
83706 (Human); 108101 (Mouse); 309186 (Rat)
Alternate Names
fermitin family member 3; FERMT3; KIND3; KIND3fermitin family homolog 3 (Drosophila); Kindlin 3; Kindlin-3; MGC10966; MIG-2; MIG2B; MIG2BURP2SF; MIG2-like protein; UNC-112 related protein 2; UNC112C; Unc-112-related protein 2; URP2; URP2fermitin family homolog 3; URP2SF

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