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Human FGF basic/FGF2/bFGF Quantikine ELISA Kit

Catalog #: DFB50
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PDFB50
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QC20: Quantikine Immunoassay Control Group 3
Human FGF basic ELISA Standard Curve
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Citations (103)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human FGF basic/FGF2/bFGF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL)
Sensitivity
3 pg/mL
Assay Range
10.0 - 640 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant human FGF basic.  This assay cross-reacts 40% with natural Bovine FGF-basic, 0.15% with natural Bovine FGF-acidic, 17% with recombinant Bovine FGF-basic, 18% with recombinant Mouse FGF-basic and 57% with recombinant Rat FGF-basic.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human FGF basic Immunoassay kit is a 4.5 hour solid phase ELISA designed to measure human FGF basic in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 28.8 104 234 32.8 108 229
Standard Deviation 2.8 3.2 7.1 3 8 17.3
CV% 9.7 3.1 3 9.1 7.4 7.6

Recovery

The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 108 94-119
EDTA Plasma (n=5) 103 90-124
Serum (n=5) 106 86-124

Linearity

To assess the linearity of the assay, the following samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and then assayed.
Human FGF basic ELISA Linearity

Scientific Data

N/A Human FGF basic ELISA Standard Curve View Larger

Human FGF basic ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name:
Fibroblast Growth Factor basic
Entrez Gene IDs:
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names:
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human FGF basic/FGF2/bFGF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

103 Citations: Showing 1 - 10
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  1. A comparative study of pathogen inactivation technologies in human platelet lysate and its optimal efficiency in human placenta-derived stem cells culture
    Authors: E Razani, HR Aghayan, S Alavi-Mogh, F Yari, A Gharehbagh, Z Sharifi
    Journal of virological methods, 2022;0(0):114478.
    Species: Human
    Sample Types: Cell Lysates
  2. Diphenylarsinic acid induced activation of MAP kinases, transcription factors, and oxidative stress-responsive factors and hypersecretion of cytokines in cultured normal human cerebellar astrocytes
    Authors: S Sasaki, T Negishi, T Tsuzuki, K Yukawa
    Neurotoxicology, 2021;88(0):196-207.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Matrix stiffness modulates hepatic stellate cell activation into tumor-promoting myofibroblasts via E2F3-dependent signaling and regulates malignant progression
    Authors: Z Liu, H Mo, R Liu, Y Niu, T Chen, Q Xu, K Tu, N Yang
    Cell Death & Disease, 2021;12(12):1134.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Circulating fibroblast growth factor-2 precipitates HIV nephropathy in mice
    Authors: JR Das, M Jerebtsova, P Tang, J Li, J Yu, PE Ray
    Disease Models & Mechanisms, 2021;14(7):.
    Species: Mouse
    Sample Types: Plasma
  5. Defined MSC exosome with high yield and purity to improve regenerative activity
    Authors: JY Kim, WK Rhim, YI Yoo, DS Kim, KW Ko, Y Heo, CG Park, DK Han
    Journal of tissue engineering, 2021;12(0):2041731421100.
    Species: Human
    Sample Types: Cell Lysates
  6. Circulating Fibroblast Growth Factor-2 precipitates HIV-nephropathy in mice
    Authors: JR Das, M Jerebtsova, P Tang, J Li, J Yu, PE Ray
    Disease Models & Mechanisms, 2021;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
    Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
    International Journal of Molecular Sciences, 2021;22(6):.
    Species: Human
    Sample Types: Plasma
  8. Repeated Freezing Procedures Preserve Structural and Functional Properties of Amniotic Membrane for Application in Ophthalmology
    Authors: O Pogozhykh, N Hofmann, O Gryshkov, C von Kaisen, M Mueller, B Glasmacher, D Pogozhykh, M Börgel, R Blasczyk, C Figueiredo
    Int J Mol Sci, 2020;21(11):.
    Species: Human
    Sample Types: Tissue Homogenates
  9. Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors
    Authors: K Hosaka, Y Yang, T Seki, Q Du, X Jing, X He, J Wu, Y Zhang, H Morikawa, M Nakamura, M Scherzer, X Sun, Y Xu, T Cheng, X Li, X Liu, Q Li, Y Liu, A Hong, Y Chen, Y Cao
    Nat Commun, 2020;11(1):3704.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. FGF signal is not required for hepatoblast differentiation of human iPS cells
    Authors: Y Toba, A Kiso, S Nakamae, F Sakurai, K Takayama, H Mizuguchi
    Sci Rep, 2019;9(1):3713.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
    Authors: ZJ Liang, X Lu, DD Zhu, XL Yi, FX Wu, N He, C Tang, CY Wei, HM Li
    Cell Transplant, 2019;0(0):9636897198256.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Collagen/Gelatin Sponges (CGSs) Provide Both Protection and Release of bFGF: An In Vitro Study
    Authors: MC Munisso, N Morimoto, SC Notodihard, T Mitsui, N Kakudo, K Kusumoto
    Biomed Res Int, 2019;2019(0):4016351.
    Species: Human
    Sample Types: Sponge Fluid
  13. Impact of HOXB7 overexpression on human adipose-derived mesenchymal progenitors
    Authors: EM Foppiani, O Candini, I Mastrolia, A Murgia, G Grisendi, AV Samarelli, G Boscaini, L Pacchioni, M Pinelli, G De Santis, EM Horwitz, E Veronesi, M Dominici
    Stem Cell Res Ther, 2019;10(1):101.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Stromal fibroblast growth factor 2 reduces the efficacy of bromodomain inhibitors in uveal melanoma
    Authors: V Chua, M Orloff, JL Teh, T Sugase, C Liao, TJ Purwin, BQ Lam, M Terai, G Ambrosini, RD Carvajal, G Schwartz, T Sato, AE Aplin
    EMBO Mol Med, 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing
    Authors: A Vijayan, S A, GSV Kumar
    Sci Rep, 2019;9(1):19165.
    Species: Human
    Sample Types: Hydrogel Supernates
  16. Circulating pro- and anti-angiogenic factors in multi-stage liver disease and hepatocellular carcinoma progression
    Authors: YY Joo, JW Jang, SW Lee, SH Yoo, JH Kwon, SW Nam, SH Bae, JY Choi, SK Yoon
    Sci Rep, 2019;9(1):9137.
    Species: Human
    Sample Types: Serum
  17. Enhanced rapamycin delivery to hemangiomas by lipid polymer nanoparticles coupled with anti-VEGFR antibody
    Authors: H Li, Y Teng, X Xu, J Liu
    Int. J. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures
    Authors: F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato
    PLoS ONE, 2018;13(9):e0203048.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. NF-?B activation is an early event of changes in gene regulation for acquiring drug resistance in human adenocarcinoma PC-9 cells
    Authors: M Fukuoka, K Yoshioka, H Hohjoh
    PLoS ONE, 2018;13(8):e0201796.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis
    Authors: CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho
    Cell Death Dis, 2018;9(3):401.
    Species: Human
    Sample Types: Synovial Fluid
  21. Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches
    Authors: C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K Rouger
    Stem Cell Res Ther, 2018;9(1):128.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Evaluation of autologous skeletal muscle-derived factors for regenerative medicine applications
    Authors: M Yoshikawa, T Nakasa, M Ishikawa, N Adachi, M Ochi
    Bone Joint Res, 2017;6(5):277-283.
    Species: Human
    Sample Types: Tissue Homogenates
  23. Comparative analyses of industrial-scale human platelet lysate preparations.
    Authors: Jan Pierce, Eric Benedetti, Amber Preslar, Pam Jacobson, Ping Jin, David F Stroncek, Jo-Anna Reems
    Transfusion, 2017;0(0):1537-2995.
    Species: Human
    Sample Types: Plasma
  24. Comparison of fibrin glue and Vicryl sutures in conjunctival autografting for pterygium surgery
    Authors: X Wang, Y Zhang, L Zhou, R Wei, L Dong
    Mol. Vis., 2017;23(0):275-285.
    Species: Human
    Sample Types: Tears
  25. Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
    Authors: Y Zhang, BJ Morgan, R Smith, CR Fellows, C Thornton, M Snow, LW Francis, IM Khan
    Sci Rep, 2017;7(1):3699.
    Species: Human
    Sample Types: Serum
  26. Prognostic impact of blood and urinary angiogenic factor levels at diagnosis and during treatment in patients with osteosarcoma: a prospective study
    Authors: MD Tabone, L Brugières, S Piperno-Ne, MA Selva, P Marec-Béra, H Pacquement, C Lervat, N Corradini, JC Gentet, R Couderc, A Chevance, C Mahier-Ait, N Entz-Werle, JY Blay, MC Le Deley
    BMC Cancer, 2017;17(1):419.
    Species: Human
    Sample Types: Serum
  27. PH20 is not expressed in murine CNS and oligodendrocyte precursor cells
    Authors: M Marella, J Ouyang, J Zombeck, C Zhao, L Huang, RJ Connor, KB Phan, MC Jorge, MA Printz, RD Paladini, AB Gelb, Z Huang, GI Frost, BJ Sugarman, L Steinman, G Wei, HM Shepard, DC Maneval, PJ Lapinskas
    Ann Clin Transl Neurol, 2017;4(3):191-211.
  28. The inhibition of FGF receptor 1 activity mediates sorafenib antiproliferative effects in human malignant pleural mesothelioma tumor-initiating cells
    Authors: A Pattarozzi, E Carra, RE Favoni, R Würth, D Marubbi, RA Filiberti, L Mutti, T Florio, F Barbieri, A Daga
    Stem Cell Res Ther, 2017;8(1):119.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
    Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
    J Transl Med, 2017;15(1):90.
    Species: Human
    Sample Types: Cell Lysates
  30. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
    Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
    Sci Rep, 2017;7(1):8484.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes
    PLoS ONE, 2016;11(12):e0168058.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. A promising scaffold with excellent cytocompatibility and pro-angiogenesis action for dental tissue engineering: Strontium-doped calcium polyphosphate
    Authors: H Qin, Z Yang, L Li, X Yang, J Liu, X Chen, X Yu
    Dent Mater J, 2016;35(2):241-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates
    Authors: Anne Fischer-Ni
    Cytotherapy, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
    Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
    J Am Heart Assoc, 2016;5(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016;6(0):29029.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Adipogenic impairment of adipose tissue-derived mesenchymal stem cells in subjects with metabolic syndrome: possible protective role of FGF2
    Authors: Wilfredo Oliva-Oliv
    J. Clin. Endocrinol. Metab, 2016;0(0):jc20162256.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Expression of Heat Shock Protein 27 in Melanoma Metastases Is Associated with Overall Response to Bevacizumab Monotherapy: Analyses of Predictive Markers in a Clinical Phase II Study
    PLoS ONE, 2016;11(5):e0155242.
    Species: Human
    Sample Types: Serum
  38. In situ normoxia enhances survival and proliferation rate of human adipose tissue-derived stromal cells without increasing the risk of tumourigenesis.
    Authors: Choi, Jane Ru, Pingguan-Murphy, Belinda, Wan Abas, Wan Abu, Yong, Kar Wey, Poon, Chi Tat, Noor Azmi, Mat Aden, Omar, Siti Zaw, Chua, Kien Hui, Xu, Feng, Wan Safwani, Wan Kama
    PLoS ONE, 2015;10(1):e0115034.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Breeding Strategy Determines Rupture Incidence in Post-Infarct Healing WARPing Cardiovascular Research.
    Authors: Deckx S, Carai P, Bateman J, Heymans S, Papageorgiou A
    PLoS ONE, 2015;10(9):e0139199.
    Species: Rat
    Sample Types: Cell Culture Supernates
  40. Mesenchymal progenitors aging highlights a miR-196 switch targeting HOXB7 as master regulator of proliferation and osteogenesis.
    Authors: Candini O, Spano C, Murgia A, Grisendi G, Veronesi E, Piccinno M, Ferracin M, Negrini M, Giacobbi F, Bambi F, Horwitz E, Conte P, Paolucci P, Dominici M
    Stem Cells, 2015;33(3):939-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells.
    Authors: Iudicone, Paola, Fioravanti, Daniela, Bonanno, Giuseppi, Miceli, Michelin, Lavorino, Claudio, Totta, Pierange, Frati, Luigi, Nuti, Marianna, Pierelli, Luca
    J Transl Med, 2014;12(0):28.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growth.
    Authors: Li D, Wei X, Xie K, Chen K, Li J, Fang J
    Br J Cancer, 2014;111(1):68-77.
    Species: Human
    Sample Types: Recombinant Protein
  43. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
    Authors: Chou M, Burnouf T, Wang T
    PLoS ONE, 2014;9(6):e99145.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Increased pericyte coverage mediated by endothelial-derived fibroblast growth factor-2 and interleukin-6 is a source of smooth muscle-like cells in pulmonary hypertension.
    Authors: Ricard N, Tu L, Le Hiress M, Huertas A, Phan C, Thuillet R, Sattler C, Fadel E, Seferian A, Montani D, Dorfmuller P, Humbert M, Guignabert C
    Circulation, 2014;129(15):1586-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Malondialdehyde mediates oxidized LDL-induced coronary toxicity through the Akt-FGF2 pathway via DNA methylation.
    Authors: Yang T, Chen Y, Chang S, Chen C, Chang P, Lu S
    J Biomed Sci, 2014;21(0):11.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. The evolution of cellular deficiency in GATA2 mutation.
    Authors: Dickinson R, Milne P, Jardine L, Zandi S, Swierczek S, McGovern N, Cookson S, Ferozepurwalla Z, Langridge A, Pagan S, Gennery A, Heiskanen-Kosma T, Hamalainen S, Seppanen M, Helbert M, Tholouli E, Gambineri E, Reykdal S, Gottfreethsson M, Thaventhiran J, Morris E, Hirschfield G, Richter A, Jolles S, Bacon C, Hambleton S, Haniffa M, Bryceson Y, Allen C, Prchal J, Dick J, Bigley V, Collin M
    Blood, 2014;123(6):863-74.
    Species: Human
    Sample Types: Serum
  47. A novel splice variant of calcium and integrin-binding protein 1 mediates protein kinase D2-stimulated tumour growth by regulating angiogenesis.
    Authors: Armacki, M, Joodi, G, Nimmagadda, S C, de Kimpe, L, Pusapati, G V, Vandoninck, S, Van Lint, J, Illing, A, Seufferlein, T
    Oncogene, 2014;33(9):1167-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
    Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
    Stem Cells, 2013;31(1):146-55.
    Species: Human
    Sample Types: Tissue Homogenates
  49. The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro.
    Authors: Li Q, Zhang A, Tao C, Li X, Jin P
    Biochem Biophys Res Commun, 2013;441(3):675-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
    Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
    BMC Cell Biol, 2013;14(0):48.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Study of the quantitative, functional, cytogenetic, and immunoregulatory properties of bone marrow mesenchymal stem cells in patients with B-cell chronic lymphocytic leukemia.
    Authors: Pontikoglou C, Kastrinaki M, Klaus M, Kalpadakis C, Katonis P, Alpantaki K, Pangalis G, Papadaki H
    Stem Cells Dev, 2013;22(9):1329-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Biomarkers for antitumor activity of bevacizumab in gastric cancer models.
    Authors: Yamashita-Kashima Y, Fujimoto-Ouchi K, Yorozu K, Kurasawa M, Yanagisawa M, Yasuno H, Mori K
    BMC Cancer, 2012;12(0):37.
    Species: Human
    Sample Types: Tissue Homogenates
  53. Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model.
    Authors: Tabata T, Petitt M, Fang-Hoover J, Rivera J, Nozawa N, Shiboski S, Inoue N, Pereira L
    Am J Pathol, 2012;181(5):1540-59.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy.
    Authors: Qin W, Zhang K, Kliethermes B, Ruhlen RL, Browne EP, Arcaro KF, Sauter ER
    BMC Cancer, 2012;12(0):100.
    Species: Human
    Sample Types: Milk
  55. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
    Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
    ALTEX, 2011;28(4):305-16.
    Species: Human
    Sample Types: Cell Lysates
  56. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
    Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
    Cardiovasc. Res., 2011;89(1):157-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer.
    Authors: Landt S, Heidecke H, Korlach S, Reuter C, Schwidde I, Barinoff J, Thill M, Sehouli J, Kummel S
    Anticancer Res., 2011;31(8):2609-15.
    Species: Human
    Sample Types: Serum
  58. Fibroblast growth factor receptors are components of autocrine signaling networks in head and neck squamous cell carcinoma cells.
    Authors: Marshall ME, Hinz TK, Kono SA, Singleton KR, Bichon B, Ware KE, Marek L, Frederick BA, Raben D, Heasley LE
    Clin. Cancer Res., 2011;17(15):5016-25.
    Species: Human
    Sample Types: Cell Lysates
  59. Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
    Authors: Ding W, Knox TR, Tschumper RC
    Blood, 2010;116(16):2984-93.
    Species: Human
    Sample Types: Platelet Poor Plasma
  60. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
    Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
    Angiogenesis, 2010;13(4):337-47.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. A urinary biomarker profile for children with HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
    Kidney Int., 2009;76(2):207-14.
    Species: Human
    Sample Types: Urine
  62. Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
    Authors: Farrow B, Berger DH, Rowley D
    J. Surg. Res., 2009;156(1):155-60.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Lenalidomide plus prednisone results in durable clinical, histopathologic, and molecular responses in patients with myelofibrosis.
    Authors: Quintas-Cardama A, Kantarjian HM, Manshouri T, Thomas D, Cortes J, Ravandi F, Garcia-Manero G, Ferrajoli A, Bueso-Ramos C, Verstovsek S
    J. Clin. Oncol., 2009;27(28):4760-6.
    Species: Human
    Sample Types: Plasma
  64. Inhibitory effect of atractylenolide I on angiogenesis in chronic inflammation in vivo and in vitro.
    Authors: Wang C, Duan H, He L
    Eur. J. Pharmacol., 2009;612(1):143-52.
    Species: Mouse
    Sample Types: Air Pouch Exudate
  65. Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
    Authors: Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP
    Biomaterials, 2008;29(24):3429-37.
    Species: Porcine
    Sample Types: Plasma
  66. Elevated fibroblast growth factor-2 increases tumor necrosis factor-alpha induced endothelial cell death in high glucose.
    Authors: Clyne AM, Zhu H, Edelman ER
    J. Cell. Physiol., 2008;217(1):86-92.
    Species: Porcine
    Sample Types: Whole Cells
  67. Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo.
    Authors: Nakano Y, Oyamada M, Dai P, Nakagami T, Kinoshita S, Takamatsu T
    Invest. Ophthalmol. Vis. Sci., 2008;49(1):93-104.
    Species: Rat
    Sample Types: Aqueous Humor
  68. Clinical correlations and prognostic relevance of HGF, VEGF AND FGF expression in Brazilian patients with non-Hodgkin lymphoma.
    Authors: Etto L, Lacerda E, Baiocchi O, Silva V, Dalboni M, Alves A, Silva M, Vettore A, Colleoni G
    Leuk. Lymphoma, 2008;49(2):257-64.
    Species: Human
    Sample Types: Serum
  69. Sca-1(+) Hematopoietic Cell-based Gene Therapy with a Modified FGF-2 Increased Endosteal/Trabecular Bone Formation in Mice.
    Authors: Hall SL, Lau KH, Chen ST, Wergedal JE, Srivastava A, Klamut H, Sheng MH, Gridley DS, Mohan S, Baylink DJ
    Mol. Ther., 2007;15(10):1881-9.
    Species: Mouse
    Sample Types: Serum
  70. Electronegative LDL impairs vascular endothelial cell integrity in diabetes by disrupting fibroblast growth factor 2 (FGF2) autoregulation.
    Authors: Lu J, Jiang W, Yang JH, Chang PY, Walterscheid JP, Chen HH, Marcelli M, Tang D, Lee YT, Liao WS, Yang CY, Chen CH
    Diabetes, 2007;57(1):158-66.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  71. Angiogenic growth factors in maternal and fetal serum in pregnancies complicated by intrauterine growth restriction.
    Authors: Wallner W, Sengenberger R, Strick R, Strissel PL, Meurer B, Beckmann MW, Schlembach D
    Clin. Sci., 2007;112(1):51-7.
    Species: Human
    Sample Types: Serum
  72. Fibroblast growth factor-2 expression is altered in lambs with increased pulmonary blood flow and pulmonary hypertension.
    Authors: Wedgwood S, Devol JM, Grobe A, Benavidez E, Azakie A, Fineman JR, Black SM
    Pediatr. Res., 2007;61(1):32-6.
    Species: Ovine
    Sample Types: Cell Culture Supernates
  73. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
    Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
    Int. J. Cancer, 2007;120(3):686-93.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
    Authors: Herrmann S, Green J, Weber T, Scharfenberger A
    Bone, 2006;39(5):1156-63.
    Species: Human
    Sample Types: Tissue Homogenates
  75. Targeted deletion of CCR2 impairs deep vein thombosis resolution in a mouse model.
    Authors: Henke PK, Pearce CG, Moaveni DM, Moore AJ, Lynch EM, Longo C, Varma M, Dewyer NA, Deatrick KB, Upchurch GR, Wakefield TW, Hogaboam C, Kunkel SL
    J. Immunol., 2006;177(5):3388-97.
    Species: Mouse
    Sample Types: Tissue Homogenates
  76. A phase I biological and pharmacologic study of the heparanase inhibitor PI-88 in patients with advanced solid tumors.
    Authors: Basche M, Gustafson DL, Holden SN, O'Bryant CL, Gore L, Witta S, Schultz MK, Morrow M, Levin A, Creese BR, Kangas M, Roberts K, Nguyen T, Davis K, Addison RS, Moore JC, Eckhardt SG
    Clin. Cancer Res., 2006;12(18):5471-80.
    Species: Human
    Sample Types: Urine
  77. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
    Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
    Glia, 2006;53(8):845-57.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease.
    Authors: Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A
    Leuk. Lymphoma, 2006;47(6):1034-40.
    Species: Human
    Sample Types: Serum
  79. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. Repair of articular cartilage defect by intraarticular administration of basic fibroblast growth factor gene, using adeno-associated virus vector.
    Authors: Hiraide A, Yokoo N, Xin KQ, Okuda K, Mizukami H, Ozawa K, Saito T
    Hum. Gene Ther., 2005;16(12):1413-21.
    Species: Rat
    Sample Types: Cell Culture Supernates
  81. Alpha-tocopheryl succinate inhibits malignant mesothelioma by disrupting the fibroblast growth factor autocrine loop: mechanism and the role of oxidative stress.
    Authors: Stapelberg M, Gellert N, Swettenham E, Tomasetti M, Witting PK, Procopio A, Neuzil J
    J. Biol. Chem., 2005;280(27):25369-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  82. Relation of various plasma growth factor levels in patients with stable angina pectoris and total occlusion of a coronary artery to the degree of coronary collaterals.
    Authors: Briguori C, Testa U, Colombo A, Petrucci E, Condorelli G, Airoldi F, Peschle C, Condorelli G
    Am. J. Cardiol., 2005;97(4):472-6.
    Species: Human
    Sample Types: Serum
  83. Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis.
    Authors: Iversen PO, Wiig H
    Clin. Cancer Res., 2005;11(19):6793-9.
    Species: Human
    Sample Types: Plasma
  84. Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension.
    Authors: Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO
    Am. J. Physiol. Heart Circ. Physiol., 2005;290(3):H978-84.
    Species: Porcine
    Sample Types: Tissue Homogenates
  85. Transplantation of transduced retinal pigment epithelium in rats.
    Authors: Saigo Y, Abe T, Hojo M, Tomita H, Sugano E, Tamai M
    Invest. Ophthalmol. Vis. Sci., 2004;45(6):1996-2004.
    Species: Rat
    Sample Types: Cell Culture Supernates
  86. A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
    Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
    Biochem. Biophys. Res. Commun., 2004;325(1):24-31.
    Species: Human
    Sample Types: Cell Lysates
  87. Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury.
    Authors: Mahmood A, Lu D, Chopp M
    J. Neurotrauma, 2004;21(1):33-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  88. Diabetes can alter the signal transduction pathways in the lens of rats.
    Authors: Zatechka DS, Kador PF, Garcia-Castineiras S, Lou MF
    Diabetes, 2003;52(4):1014-22.
    Species: Rat
    Sample Types: Vitreous Humor
  89. beta-Catenin regulates vascular endothelial growth factor expression in colon cancer.
    Authors: Easwaran V, Lee SH, Inge L, Guo L, Goldbeck C, Garrett E, Wiesmann M, Garcia PD, Fuller JH, Chan V, Randazzo F, Gundel R, Warren RS, Escobedo J, Aukerman SL, Taylor RN, Fantl WJ
    Cancer Res., 2003;63(12):3145-53.
    Species: Mouse
    Sample Types: Serum
  90. Low-density lipoprotein in hypercholesterolemic human plasma induces vascular endothelial cell apoptosis by inhibiting fibroblast growth factor 2 transcription.
    Authors: Chen CH, Jiang T, Yang JH, Jiang W, Lu J, Marathe GK, Pownall HJ, Ballantyne CM, McIntyre TM, Henry PD, Yang CY
    Circulation, 2003;107(16):2102-8.
    Species: Bovine
    Sample Types: Cell Lysates
  91. Have urinary levels of the angiogenic factors, basic fibroblast growth factor and vascular endothelial growth factor, a prognostic value in childhood acute lymphoblastic leukaemia?
    Authors: Schneider P, Vasse M, Legrand E, Callat MP, Vannier JP
    Br. J. Haematol., 2003;122(1):163-4.
    Species: Human
    Sample Types: Urine
  92. High basic fibroblast growth factor levels in nipple aspirate fluid are correlated with breast cancer.
    Authors: Hsiung R, Zhu W, Klein G, Qin W, Rosenberg A, Park P, Rosato E, Sauter E
    Cancer J, 2002;8(4):303-10.
    Species: Human
    Sample Types: Tissue Secretion
  93. Circadian variations of transforming growth factor-beta2 and basic fibroblast growth factor in the rabbit aqueous humor.
    Authors: Liu JH
    Curr. Eye Res., 2002;24(1):75-80.
    Species: Rabbit
    Sample Types: Aqueous Humor
  94. Retinoscleral control of scleral remodelling in refractive development: a role for endogenous FGF-2?
    Authors: Gentle A, { –!áà§S|†, McBrien NA
    , 2002;18(6):344-8.
    Species: Tree Shrew
    Sample Types: Tissue Homogenates
  95. Increase in gap junctional intercellular communication by high molecular weight hyaluronic acid associated with fibroblast growth factor 2 and keratinocyte growth factor production in normal human dermal fibroblasts.
    Authors: Park JU, Tsuchiya T
    Tissue Eng., 2002;8(3):419-27.
    Species: Human
    Sample Types: Cell Culture Supernates
  96. Basic fibroblast growth factor in human saliva decreases with aging.
    Authors: Westermark A, Pyykko I, Magnusson M, Ishizaki H, Jantti P, van Setten G
    Laryngoscope, 2002;112(5):887-9.
    Species: Human
    Sample Types: Saliva
  97. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
    Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
    Exp. Neurol., 2002;175(0):318.
    Species: Rat
    Sample Types: Cell Culture Supernates
  98. Sugar-induced modification of fibroblast growth factor 2 reduces its angiogenic activity in vivo.
    Authors: Facchiano F, Lentini A, Fogliano V, Mancarella S, Rossi C, Facchiano A, Capogrossi MC
    Am. J. Pathol., 2002;161(2):531-41.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  99. Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts.
    Authors: Skutek M, van Griensven M, Zeichen J, Brauer N, Bosch U
    Eur. J. Appl. Physiol., 2001;86(1):48-52.
    Species: Human
    Sample Types: Cell Culture Supernates
  100. Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo.
    Authors: Westphal JR, Van't Hullenaar R, Peek R, Willems RW, Crickard K, Crickard U, Askaa J, Clemmensen I, Ruiter DJ, De Waal RM
    Int. J. Cancer, 2000;86(6):768-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  101. Intestinal mucosal secretion of basic fibroblast growth factor in patients with ulcerative colitis.
    Authors: Thorn M, Raab Y, Larsson A, Gerdin B, Hallgren R
    Scand. J. Gastroenterol., 2000;35(4):408-12.
    Species: Human
    Sample Types: Tissue Perfusion Fluid
  102. Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome.
    Authors: Soubrier M, Sauron C, Souweine B, Larroche C, Wechsler B, Guillevin L, Piette JC, Rousset H, Deteix P
    Am. J. Kidney Dis., 1999;34(4):633-8.
    Species: Human
    Sample Types: Serum
  103. Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
    Authors: Gu JW, Adair TH
    Am. J. Physiol., 1997;273(2):H628-33.
    Species: Canine
    Sample Types: Cell Culture Supernates

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