Human FGF-BP DuoSet ELISA

R&D Systems | Catalog # DY1593-05

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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

23.4-1500 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human FGF-BP DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all FGF-BP ELISA Kits »

Product Summary for Human FGF-BP DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human FGF-BP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human FGF-BP DuoSet ELISA

Human FGF-BP ELISA Standard Curve

Human FGF-BP ELISA Standard Curve

Kit Contents for Human FGF-BP DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-BP

FGF binding protein (FGF-BP) was originally identified as a 17 kDa low-affinity heparin-binding protein in the conditioned medium of human A431 epidermoid carcinoma cells that bind FGF acidic and basic in a non-covalent, reversible manner. Rat FGF-BP cDNA encodes a 238 amino acid residue (aa) precursor protein with a 33 aa signal peptide. All FGF-BPs share ten conserved cysteine residues which form five intracellular disufide bridges.

Long Name

Fibroblast Growth Factor Binding Protein

Alternate Names

FGF-BP1, FGFBP, FGFBP-1, FGFBP1, HBp17

Entrez Gene IDs

9982 (Human); 14181 (Mouse); 64535 (Rat)

Gene Symbol

FGFBP1

Additional FGF-BP Products

Product Documents for Human FGF-BP DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FGF-BP DuoSet ELISA

For research use only

Related Research Areas

Developmental Biology
FGF Family

Citations for Human FGF-BP DuoSet ELISA

Customer Reviews for Human FGF-BP DuoSet ELISA (2)

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  • Human FGF-BP DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 05/16/2022
    We used this kit to quantify FGF-BP in human serum and plasma, need more serum/plasma sample.
    Human FGF-BP DuoSet ELISA DY1593-05
  • Human FGF-BP DuoSet ELISA
    Name: Jeff Pimentel
    Sample Tested: Serum
    Verified Customer | Posted 11/02/2018

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