Human Fibroblast Activation Protein  alpha /FAP Biotinylated Antibody

(1 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Fibroblast Activation Protein alpha /FAP in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein  alpha /FAP
    Leu26-Asp760
    Accession # Q12884
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
    • Human Fibroblast Activation Protein alpha /FAP Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human Fibroblast Activation Protein  alpha /FAP Antibody (Catalog # MAB3715)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Fibroblast Activation Protein  alpha /FAP Biotinylated Antibody (Catalog # BAF3715)
  • ELISA Standard
     
    Recombinant Human FAP Protein, CF (Catalog # 3715-SE)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Fibroblast Activation Protein  alpha /FAP by Western Blot. Western blot shows lysates of WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein  alpha /FAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3715) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for Fibroblast Activation Protein  alpha /FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.

  • References:
    1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
    2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
    3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
    4. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
    5. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
  • Entrez Gene IDs:
    2191 (Human); 14089 (Mouse)
  • Alternate Names:
    170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Cancer associated fibroblasts in pancreatic cancer are reprogrammed by tumor-induced alterations in genomic DNA methylation
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: cell separation
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Biotinylated Control

Ctrl BAF020 1
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human Fibroblast Activation Protein  alpha /FAP Biotinylated Antibody BAF3715
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 Flow Mouse Anonymous 11/10/2017
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Flow Cytometry Human Fibroblast Activation Protein  alpha /FAP Biotinylated Antibody BAF3715
Flow Cytometry: Human Fibroblast Activation Protein  alpha /FAP Biotinylated Antibody [BAF3715]

Summary

ApplicationFlow Cytometry
Sample TestedTumor cell lyastes
SpeciesMouse

Other Experimental Details

Other Experimental DetailsSince the mouse FAP shares ~90% of the human protein sequence, in our hands this antibody works good also mouse cells.We are using it for flow cytometry on primary mouse cells in combination with APC Streptavidin (Biolegend, 405207). The staining works properly with a concentration of 1:20 of FAP Biotinylated Antibody and 1:100 of APC Streptavidin.

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