Detects human Follistatin in direct ELISAs and Western blots. It recognizes the 288 aa, 300 aa, and 315 aa isoforms of human Follistatin. In direct ELISAs and Western blots, this antibody shows approximately 50% cross-reactivity with recombinant mouse Follistatin.
Monoclonal Mouse IgG2A Clone # 85918
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Follistatin Gly30-Asp329 Accession # P19883
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human Follistatin 300 aa 30-329 (Catalog # 669-FO) under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Follistatin in Human Pituitary. Follistatin was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Follistatin Monoclonal Antibody (Catalog # MAB669) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(FST) is a secreted glycoprotein that was first identified as a
follicle-stimulating hormone inhibiting substance in ovarian follicular
fluid (1, 2). Human Follistatin cDNA encodes a 344 amino acid (aa)
protein with a 29 aa signal sequence, an N-terminal atypical TGF binding
domain, three Follistatin domains that contain EGF-like and kazal-like
motifs, and a highly acidic C-terminal tail. The first Follistatin
domain (FS1) contains a heparin binding site, while FS1 and FS2 are most
critical for activin binding and neutralization (3, 4). In addition to
activin, Follistatin regulates bioavailability of many non-TGF-beta members
of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). It also
regulates hematopoietic stem cell adhesion to fibronectin via FS2, and
binds angiogenin via FS2 and FS3 (6, 7). Some Follistatin binding
partners will also bind Follistatin-like proteins such as FSL-3
(3, 5, 6). Of three Follistatin isoforms, the full-length mature
Follistatin (FST315) is the most abundant and the sole form in plasma,
but has lower binding affinity for both activins and heparins than
alternative isoforms (5, 8, 9). The acidic tail is missing in the splice
variant FST288 which shows the highest affinity for activins, while
a partial tail exists in the proteolytically produced FST303, which
shows intermediate activin affinity (5, 8, 9). FST315 shares 98% aa
identity with mouse, rat, equine and ovine FST, 99% with porcine and 97%
with bovine FST. Genetic deletion of Follistatin in mice, or expression
of only the FST288 form, is perinatally lethal due to defects of lung,
skin and musculoskeletal system (10). Expression of only the FST315
isoform allows survival, with defects in vascularization and female
Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218.
Thompson, T.B. et al. (2005) Dev. Cell 9:535.
Sidis, Y. et al. (2005) Endocrinology 146:130.
Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228.
Sidis, Y. et al. (2006) Endocrinology 147:3586.
Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
Gao, X. et al. (2007) FEBS Lett. 581:5505.
Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.