Human Follistatin Antibody

Catalog # Availability Size / Price Qty
MAB669-SP
MAB669-100
MAB669-500
Follistatin in Human Pituitary.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (2)

Human Follistatin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Follistatin in direct ELISAs and Western blots. It recognizes the 288 aa, 300 aa, and 315 aa isoforms of human Follistatin. In direct ELISAs and Western blots, this antibody shows approximately 50% cross-reactivity with recombinant mouse Follistatin.
Source
Monoclonal Mouse IgG2A Clone # 85918
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Follistatin
Gly30-Asp329
Accession # P19883
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Follistatin 300 aa 30-329 (Catalog # 669-FO)
under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Follistatin antibody in Human Pituitary by Immunohistochemistry (IHC-P). View Larger

Follistatin in Human Pituitary. Follistatin was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Follistatin Monoclonal Antibody (Catalog # MAB669) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin

Follistatin (FST) is a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human Follistatin cDNA encodes a 344 amino acid (aa) protein with a 29 aa signal sequence, an N-terminal atypical TGF binding domain, three Follistatin domains that contain EGF-like and kazal-like motifs, and a highly acidic C-terminal tail. The first Follistatin domain (FS1) contains a heparin binding site, while FS1 and FS2 are most critical for activin binding and neutralization (3, 4). In addition to activin, Follistatin regulates bioavailability of many non-TGF-beta members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). It also regulates hematopoietic stem cell adhesion to fibronectin via FS2, and binds angiogenin via FS2 and FS3 (6, 7). Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3 (3, 5, 6). Of three Follistatin isoforms, the full-length mature Follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative isoforms (5, 8, 9). The acidic tail is missing in the splice variant FST288 which shows the highest affinity for activins, while a partial tail exists in the proteolytically produced FST303, which shows intermediate activin affinity (5, 8, 9). FST315 shares 98% aa identity with mouse, rat, equine and ovine FST, 99% with porcine and 97% with bovine FST. Genetic deletion of Follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility (10).

References
  1. Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218. 
  2. Thompson, T.B. et al. (2005) Dev. Cell 9:535. 
  3. Sidis, Y. et al. (2005) Endocrinology 146:130. 
  4. Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228. 
  5. Sidis, Y. et al. (2006) Endocrinology 147:3586. 
  6. Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
  7. Gao, X. et al. (2007) FEBS Lett. 581:5505.
  8. Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
  9. Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
  10. Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.
Entrez Gene IDs
10468 (Human); 14313 (Mouse)
Alternate Names
follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST

Product Datasheets

Citations for Human Follistatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Follistatin impacts Tumor Angiogenesis and Outcome in Thymic Epithelial Tumors
    Authors: S Janik, C Bekos, P Hacker, T Raunegger, AI Schiefer, L Müllauer, C Veraar, B Dome, W Klepetko, HJ Ankersmit, B Moser
    Sci Rep, 2019;9(1):17359.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Differentiation of nestin?negative human hair follicle outer root sheath cells into neurons in�vitro
    Authors: W Wu, XL Wu, YQ Ji, Z Gao
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. BMP-driven NRF2 activation in esophageal basal cell differentiation and eosinophilic esophagitis.
    Authors: Jiang, Ming, Ku, Wei-Yao, Zhou, Zhongren, Dellon, Evan S, Falk, Gary W, Nakagawa, Hiroshi, Wang, Mei-Lun, Liu, Kuancan, Wang, Jun, Katzka, David A, Peters, Jeffrey, Lan, Xiaopeng, Que, Jianwen
    J Clin Invest, 2015;125(4):1557-68.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Canine follicle stem cell candidates reside in the bulge and share characteristic features with human bulge cells.
    Authors: Kobayashi T, Iwasaki T, Amagai M
    J. Invest. Dermatol., 2010;130(8):1988-95.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Nucleolar follistatin promotes cancer cell survival under glucose-deprived conditions through inhibiting cellular rRNA synthesis.
    Authors: Gao X, Wei S, Lai K
    J. Biol. Chem., 2010;285(47):36857-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. The activin-follistatin system and in vitro early follicle development in goats.
    Authors: Silva JR, Tharasanit T, Taverne MA, van der Weijden GC, Santos RR, Figueiredo JR, van den Hurk R
    J. Endocrinol., 2006;189(1):113-25.
    Species: Goat
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Thapsigargin resistance in human prostate cancer cells.
    Authors: O'Neill JP, Velalar CN, Lee DI, Zhang B, Nakanishi T, Tang Y, Selaru F, Ross D, Meltzer SJ, Hussain A
    Cancer, 2006;107(3):649-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts.
    Authors: Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J
    Arthritis Rheum., 2004;50(8):2521-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells.
    Authors: Abe Y, Abe T, Aida Y, Hara Y, Maeda K
    J. Bone Miner. Res., 2004;19(8):1302-7.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization

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Isotype Controls

Mouse IgG2A Isotype Control

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Secondary Antibodies

Reviews for Human Follistatin Antibody

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Human Follistatin Antibody
By Anonymous on 10/19/2021
Application: IHC Sample Tested: ductal carcinoma Species: Human

Human Follistatin Antibody
By Anonymous on 08/20/2021
Application: IHC Sample Tested: Liver tissue Species: Human