Human Follistatin Antibody

(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Follistatin in direct ELISAs and Western blots. It recognizes the 288 aa, 300 aa, and 315 aa isoforms of human Follistatin. In direct ELISAs and Western blots, this antibody shows approximately 50% cross-reactivity with recombinant mouse Follistatin.
  • Source
    Monoclonal Mouse IgG2A Clone # 85918
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Follistatin
    Gly30-Asp329
    Accession # P19883
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Follistatin 300 aa 30-329 (Catalog # 669-FO)
    under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Follistatin in Human Pituitary. Follistatin was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Follistatin Monoclonal Antibody (Catalog # MAB669) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Follistatin
Follistatin (FST) is a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human Follistatin cDNA encodes a 344 amino acid (aa) protein with a 29 aa signal sequence, an N-terminal atypical TGF binding domain, three Follistatin domains that contain EGF-like and kazal-like motifs, and a highly acidic C-terminal tail. The first Follistatin domain (FS1) contains a heparin binding site, while FS1 and FS2 are most critical for activin binding and neutralization (3, 4). In addition to activin, Follistatin regulates bioavailability of many non-TGF-beta members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). It also regulates hematopoietic stem cell adhesion to fibronectin via FS2, and binds angiogenin via FS2 and FS3 (6, 7). Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3 (3, 5, 6). Of three Follistatin isoforms, the full-length mature Follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative isoforms (5, 8, 9). The acidic tail is missing in the splice variant FST288 which shows the highest affinity for activins, while a partial tail exists in the proteolytically produced FST303, which shows intermediate activin affinity (5, 8, 9). FST315 shares 98% aa identity with mouse, rat, equine and ovine FST, 99% with porcine and 97% with bovine FST. Genetic deletion of Follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility (10).
  • References:
    1. Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218. 
    2. Thompson, T.B. et al. (2005) Dev. Cell 9:535. 
    3. Sidis, Y. et al. (2005) Endocrinology 146:130. 
    4. Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228. 
    5. Sidis, Y. et al. (2006) Endocrinology 147:3586. 
    6. Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
    7. Gao, X. et al. (2007) FEBS Lett. 581:5505.
    8. Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
    9. Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
    10. Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.
  • Entrez Gene IDs:
    10468 (Human); 14313 (Mouse)
  • Alternate Names:
    follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Differentiation of nestin?negative human hair follicle outer root sheath cells into neurons in�vitro
    Authors: W Wu, XL Wu, YQ Ji, Z Gao
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. BMP-driven NRF2 activation in esophageal basal cell differentiation and eosinophilic esophagitis.
    Authors: Jiang, Ming, Ku, Wei-Yao, Zhou, Zhongren, Dellon, Evan S, Falk, Gary W, Nakagawa, Hiroshi, Wang, Mei-Lun, Liu, Kuancan, Wang, Jun, Katzka, David A, Peters, Jeffrey, Lan, Xiaopeng, Que, Jianwen
    J Clin Invest, 2015;125(4):1557-68.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Canine follicle stem cell candidates reside in the bulge and share characteristic features with human bulge cells.
    Authors: Kobayashi T, Iwasaki T, Amagai M
    J. Invest. Dermatol., 2010;130(8):1988-95.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Nucleolar follistatin promotes cancer cell survival under glucose-deprived conditions through inhibiting cellular rRNA synthesis.
    Authors: Gao X, Wei S, Lai K
    J. Biol. Chem., 2010;285(47):36857-64.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Thapsigargin resistance in human prostate cancer cells.
    Authors: O'Neill JP, Velalar CN, Lee DI, Zhang B, Nakanishi T, Tang Y, Selaru F, Ross D, Meltzer SJ, Hussain A
    Cancer, 2006;107(3):649-59.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. The activin-follistatin system and in vitro early follicle development in goats.
    Authors: Silva JR, Tharasanit T, Taverne MA, van der Weijden GC, Santos RR, Figueiredo JR, van den Hurk R
    J. Endocrinol., 2006;189(1):113-25.
    Species: Goat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts.
    Authors: Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J
    Arthritis Rheum., 2004;50(8):2521-30.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells.
    Authors: Abe Y, Abe T, Aida Y, Hara Y, Maeda K
    J. Bone Miner. Res., 2004;19(8):1302-7.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
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