Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 553701
View all FOLR3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FOLR3 long isoform
Ala22-Ser245
Accession # NP_000795

Specificity

Detects human FOLR3 in direct ELISAs and Western blots. In direct ELISAs, 10% cross-reactivity with recombinant human (rh) FOLR2 is observed and no cross-reactivity with rhFOLR4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FOLR3 Antibody

Detection of Human FOLR3 by Western Blot.

Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FOLR3 Monoclonal Antibody (Catalog # MAB5319) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for FOLR3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 2.

Applications for Human FOLR3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: JEG‑3 human epithelial choriocarcinoma cell line

Reviewed Applications

Read 2 reviews rated 4 using MAB5319 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FOLR3

Folate receptor 3 (FOLR3), also known as folate receptor gamma, is a 32 kDa protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1). Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence (2, 3). A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein (2). Mature human FOLR3 shares 74% and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139 aa (2, 4). FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas (2, 3). FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas (5). Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates (3).

References

  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Shen, F. et al. (1994) Biochemistry 33:1209.
  3. Shen, F. et al. (1995) Biochemistry 34:5660.
  4. Wang, H. et al. (1998) Nucl. Acids Res. 26:2132.
  5. Yuan, Y. et al. (2009) Hum. Pathol. May 18 epub.

Long Name

Folate Receptor 3

Alternate Names

FR-gamma

Entrez Gene IDs

2352 (Human)

Gene Symbol

FOLR3

UniProt

NP_000795

Additional FOLR3 Products

Product Documents for Human FOLR3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FOLR3 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Customer Reviews for Human FOLR3 Antibody (2)

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  • Human FOLR3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human FOLR3 Antibody MAB5319
  • Human FOLR3 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018

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