Human FOLR3 Antibody Summary
Accession # P41439
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of FOLR3 in KG‑1 Human Cell Line by Flow Cytometry. KG-1 human acute myelogenous leukemia cell line was stained with Sheep Anti-Human FOLR3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5319, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human FOLR3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FOLR3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5319) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Folate receptor 3 (FOLR3), also known as folate receptor gamma, is a 32 kDa protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1). Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence (2, 3). A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein (2). Mature human FOLR3 shares 74% and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139 aa (2, 4). FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas (2, 3). FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas (5). Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates (3).
- Fowler, B. et al. (2001) Kidney Int. 59:S-221.
- Shen, F. et al. (1994) Biochemistry 33:1209.
- Shen, F. et al. (1995) Biochemistry 34:5660.
- Wang, H. et al. (1998) Nucl. Acids Res. 26:2132.
- Yuan, Y. et al. (2009) Hum. Pathol. May 18 epub.
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