Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FOLR3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FOLR3 long isoform
Ala20-Ser243
Accession # P41439

Specificity

Detects human FOLR3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) FOLR2 is observed and less than 1% cross-reactivity with rhFOLR1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FOLR3 Antibody

Detection of Human FOLR3 by Western Blot.

Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FOLR3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5319) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for FOLR3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of FOLR3 antibody in KG-1 Human Cell Line antibody by Flow Cytometry.

Detection of FOLR3 in KG‑1 Human Cell Line by Flow Cytometry.

KG-1 human acute myelogenous leukemia cell line was stained with Sheep Anti-Human FOLR3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5319, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human FOLR3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: KG‑1 human acute myelogenous leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: JEG‑3 human epithelial choriocarcinoma cell line

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FOLR3

Folate receptor 3 (FOLR3), also known as folate receptor gamma, is a 32 kDa protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1). Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence (2, 3). A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein (2). Mature human FOLR3 shares 74% and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139 aa (2, 4). FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas (2, 3). FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas (5). Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates (3).

References

  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Shen, F. et al. (1994) Biochemistry 33:1209.
  3. Shen, F. et al. (1995) Biochemistry 34:5660.
  4. Wang, H. et al. (1998) Nucl. Acids Res. 26:2132.
  5. Yuan, Y. et al. (2009) Hum. Pathol. May 18 epub.

Long Name

Folate Receptor 3

Alternate Names

FR-gamma

Entrez Gene IDs

2352 (Human)

Gene Symbol

FOLR3

UniProt

P41439

Additional FOLR3 Products

Product Documents for Human FOLR3 Antibody

Certificate of Analysis

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Product Specific Notices for Human FOLR3 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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