Human FoxO1/FKHR Antibody (597554) [Alexa Fluor® 594]

R&D Systems | Catalog # FAB5939T

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 597554
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Product Specifications

Immunogen

E. coli- derived recombinant human FoxO1/FKHR
Ala353-Gly655
Accession # Q12778

Specificity

Detects human FoxO1/FKHR in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FoxA1 (aa 253-472), B1 (aa 191-324), C1 (aa 208-322), D3 (aa 1-140), F1 (aa 129-354), G1 (aa 283-489), J1 (aa 301-421), J3 (aa 375-552), K1 (aa 493-670

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human FoxO1/FKHR Antibody (597554) [Alexa Fluor® 594]

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months, 2 to 8 °C under sterile conditions after opening.
  • Background: FoxO1/FKHR

    FoxO1, also called FKHR (forkhead in ­rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity. A 60 kDa form, cleaved at R537, has been found in androgen-treated prostate cancer cells. Over aa 353-655, human FoxO1 shares 93% aa identity with mouse and rat FoxO1.

    Long Name

    Forkhead Box O1

    Alternate Names

    FKH1, FKHR

    Entrez Gene IDs

    2308 (Human); 56458 (Mouse); 84482 (Rat)

    Gene Symbol

    FOXO1

    UniProt

    Additional FoxO1/FKHR Products

    Product Documents for Human FoxO1/FKHR Antibody (597554) [Alexa Fluor® 594]

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Human FoxO1/FKHR Antibody (597554) [Alexa Fluor® 594]


    This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

    For research use only

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