Human Frk Antibody Summary
Accession # P42685
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Frk in Human Breast Cancer Tissue. Frk was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Rat Anti-Human Frk Monoclonal Antibody (Catalog # MAB3766) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus of epithelial cells in interlobular ducts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fyn-related kinase (Frk) is a tyrosine kinase of the Src family. Also known as Rak, Gtk, and Iyk, Frk is predominantly expressed in epithelial tissues. Frk protein is 49% and 47% identical to Fyn and Csk, respectively. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.
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