Human Frk Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Frk in direct ELISAs.
  • Source
    Monoclonal Rat IgG1 Clone # 393812
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Frk
    Met1-Glu107
    Accession # P42685
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Frk in Human Breast Cancer Tissue. Frk was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Rat Anti-Human Frk Monoclonal Antibody (Catalog # MAB3766) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus of epithelial cells in interlobular ducts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frk
Fyn-related kinase (Frk) is a tyrosine kinase of the Src family. Also known as Rak, Gtk, and Iyk, Frk is predominantly expressed in epithelial tissues. Frk protein is 49% and 47% identical to Fyn and Csk, respectively. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.
  • Long Name:
    FYN-related Kinase
  • Entrez Gene IDs:
    2444 (Human); 14302 (Mouse); 79209 (Rat)
  • Alternate Names:
    EC 2.7.10; Frk; fyn-related kinaseProtein-tyrosine kinase 5; Gtk; GTKEC 2.7.10.2; Nuclear tyrosine protein kinase RAK; PTK5 protein tyrosine kinase 5; PTK5; Rak; RAKPTK5; tyrosine-protein kinase FRK
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Rat IgG1 Isotype Control

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Goat Anti-Rat IgG Biotinylated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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