Detection of Human FUBI/MNSF beta by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FUBI/MNSF beta at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FUBI/MNSF beta in HEK293 Human Cell Line.
FUBI/MNSF beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasmic puncta. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FUBI/MNSF beta
The ubiquitin-like protein FUBI/ MNSF beta (Monoclonal Nonspecific Suppressor Factor Beta) is encoded by the FAU gene, and is translated as a pro-form consisting of FUBI fused to the ribosomal S30 protein. S30 is cleaved in a post-translational reaction, releasing the mature 74 amino acid FUBI/MNSF beta protein. FUBI has a C-terminal gly-gly motif common to many ubiquitin-like proteins. FUBI/ MNSF beta conjugation to Bcl-G has been shown to regulate the ERK1/2-MAPK cascade in macrophage cell lines, and may be implicated in TLR4-mediated signal transduction. Conjugation to endophilin II regulates dectin-1-mediated phagocytosis and inflammatory responses, and may be implicated in TLR2 signaling pathway.
Rossman,T.G. et al. (2003) Oncogene 22:1817.
Nakamura, M. & Omura, S. (2008) Biosci. Biotech. Biochem. 72:1915.
Nakamura, M. & Yamaguchi, S. (2006) J. Biol. Chem. 281:16861.
Nakamura, M & Watanabe, N. (2010) Biochem, Biophys. Res. Comm. 15:275.
Nakamura, M. et al. (2012) Mol. Cell Biochem. 364:39.
Entrez Gene IDs:
2197 (Human); 100039316 (Mouse); 29752 (Rat)
40S Ribosomal Protein S30; asr1; FAU; FAU1; FAU-encoded ubiquitin-like protein; FBR-MuSV-associated ubiquitously expressed; Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed; Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed(fox derived); FLJ22986; Fub1; FUBI; MNSFbeta; monoclonal nonspecific suppressor factor beta; ribosomal protein S30; RPS30; S30; ubiquitin-like protein fubi and ribosomal protein S30; ubiquitin-like-S30 fusion protein
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