Human FUBI/MNSF beta Antibody (861504)

R&D Systems | Catalog # MAB9036

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 861504
View all FUBI/MNSF beta Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human FUBI/MNSF beta
Met1-Gly74
Accession # P35544

Specificity

Detects human FUBI in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human FUBI/MNSF beta Antibody (861504)

Detection of Human FUBI/MNSF beta antibody by Western Blot.

Detection of Human FUBI/MNSF beta by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FUBI/MNSF beta at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FUBI/MNSF beta antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

FUBI/MNSF beta in HEK293 Human Cell Line.

FUBI/MNSF beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasmic puncta. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FUBI/MNSF beta Antibody (861504)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FUBI/MNSF beta

The ubiquitin-like protein FUBI/ MNSF beta (Monoclonal Nonspecific Suppressor Factor Beta) is encoded by the FAU gene, and is translated as a pro-form consisting of FUBI fused to the ribosomal S30 protein. S30 is cleaved in a post-translational reaction, releasing the mature 74 amino acid FUBI/MNSF beta protein. FUBI has a
C-terminal gly-gly motif common to many ubiquitin-like proteins.  FUBI/ MNSF beta conjugation to Bcl-G has been shown to regulate the ERK1/2-MAPK cascade in macrophage cell lines, and may be implicated in TLR4-mediated signal transduction. Conjugation to endophilin II regulates dectin-1-mediated phagocytosis and inflammatory responses, and may be implicated in TLR2 signaling pathway.

References

  1. Rossman,T.G. et al. (2003) Oncogene 22:1817.
  2. Nakamura, M. & Omura, S. (2008) Biosci. Biotech. Biochem. 72:1915.
  3. Nakamura, M. & Yamaguchi, S. (2006) J. Biol. Chem. 281:16861.
  4. Nakamura, M & Watanabe, N. (2010) Biochem, Biophys. Res. Comm. 15:275.
  5. Nakamura, M. et al. (2012) Mol. Cell Biochem. 364:39.

Alternate Names

40S Ribosomal Protein S30, asr1, FAU, Fub1, MNSFbeta, RPS30, S30

Entrez Gene IDs

2197 (Human); 100039316 (Mouse); 29752 (Rat)

Gene Symbol

FAU

UniProt

P35544

Additional FUBI/MNSF beta Products

Product Documents for Human FUBI/MNSF beta Antibody (861504)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FUBI/MNSF beta Antibody (861504)

For research use only

Citations for Human FUBI/MNSF beta Antibody (861504)

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Protocols

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