Human FUBI/MNSF beta Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FUBI in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 861504
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human FUBI/MNSF beta
    Met1-Gly74
    Accession # P35544
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FUBI/MNSF beta by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FUBI/MNSF beta at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
FUBI/MNSF beta in HEK293 Human Cell Line. FUBI/MNSF beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody
(Catalog # MAB9036) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasmic puncta. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FUBI/MNSF beta
The ubiquitin-like protein FUBI/ MNSF beta (Monoclonal Nonspecific Suppressor Factor Beta) is encoded by the FAU gene, and is translated as a pro-form consisting of FUBI fused to the ribosomal S30 protein. S30 is cleaved in a post-translational reaction, releasing the mature 74 amino acid FUBI/MNSF beta protein. FUBI has a
C-terminal gly-gly motif common to many ubiquitin-like proteins.  FUBI/ MNSF beta conjugation to Bcl-G has been shown to regulate the ERK1/2-MAPK cascade in macrophage cell lines, and may be implicated in TLR4-mediated signal transduction. Conjugation to endophilin II regulates dectin-1-mediated phagocytosis and inflammatory responses, and may be implicated in TLR2 signaling pathway.

  • References:
    1. Rossman,T.G. et al. (2003) Oncogene 22:1817.
    2. Nakamura, M. & Omura, S. (2008) Biosci. Biotech. Biochem. 72:1915.
    3. Nakamura, M. & Yamaguchi, S. (2006) J. Biol. Chem. 281:16861.
    4. Nakamura, M & Watanabe, N. (2010) Biochem, Biophys. Res. Comm. 15:275.
    5. Nakamura, M. et al. (2012) Mol. Cell Biochem. 364:39.
  • Entrez Gene IDs:
    2197 (Human); 100039316 (Mouse); 29752 (Rat)
  • Alternate Names:
    40S Ribosomal Protein S30; asr1; FAU; FAU1; FAU-encoded ubiquitin-like protein; FBR-MuSV-associated ubiquitously expressed; Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed; Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed(fox derived); FLJ22986; Fub1; FUBI; MNSFbeta; monoclonal nonspecific suppressor factor beta; ribosomal protein S30; RPS30; S30; ubiquitin-like protein fubi and ribosomal protein S30; ubiquitin-like-S30 fusion protein
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