Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
31.2-2000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human G-CSF DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Background: G-CSF
Granulocyte-colony
stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates
the proliferation, differentiation, and activation of hematopoietic cells in the
neutrophilic granulocyte lineage. Mature human G-CSF is a 178 amino acid (aa) O-glycosylated
protein that contains two intrachain disulfide bridges. In humans, alternate
splicing generates a second minor isoform with a 3 aa deletion. Mouse and human
G-CSF share 76% aa sequence identity, and the two proteins show species
cross-reactivity. G-CSF is produced by activated monocytes and macrophages,
fibroblasts, endothelial cells, astrocytes, neurons, and bone marrow stroma
cells. In addition, various tumor cells express G-CSF constitutively.
Human G-CSF
receptor (G-CSF R) is a 120 kDa type I transmembrane glycoprotein that belongs to the
hematopoietin receptor superfamily. The mature protein consists of a 603 aa extracellular
domain (ECD), a 23 aa transmembrane segment, and a 186 aa cytoplasmic domain.
The ECD contains an N-terminal Ig-like domain, a cytokine receptor homology
domain, and three fibronectin type III domains. Alternate splicing of human
G-CSF R generates additional isoforms including a potentially soluble form of
the receptor. The ECDs of mouse and human G-CSF R share 63% aa sequence identity.
G-CSF R forms a complex with the ligand in a 2:2 ratio. It is expressed on
monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors,
trophoblasts and placenta, endothelial cells, and various tumor cell types.
G-CSF is an
important regulator for granulopoiesis in vivo, and mutations in G-CSF R are associated
with congenital neutropenia. G-CSF can support the growth of multilineage
hematopoietic progenitor cells and mobilize them from the bone marrow into the bloodstream.
G-CSF enhances the functional capacity of mature neutrophils and supports their
survival by limiting the rate of apoptosis. G-CSF also enhances M-CSF
induced monocytopoiesis from hematopoietic progenitor cells and stimulates the proliferation
of peripheral Th2-inducing dendritic cells (30, 31). It promotes the
development of T cell immune tolerance as well as tissue recovery following
myocardial infarction and cerebral ischemia.
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
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