Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GADD45 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GADD45 alpha
Met1-Arg165
Accession # P24522

Specificity

Detects human GADD45 alpha in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human GADD45 alpha Antibody

Detection of Human GADD45a antibody by Western Blot.

Detection of Human GADD45 alpha by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with full length human GADD45a. PVDF membrane was probed with 1 µg/mL Human GADD45a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5510) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for GADD45a was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GADD45 alpha Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line transfected with full length human GADD45 alpha

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GADD45 alpha

GADD45 alpha (Growth arrest and DNA damage-inducible protein 45 alpha) is a 19 kDa member of the GADD45 family of proteins. It is ubiquitously expressed, and induced by DNA damage. Upon induction, GADD45 alpha binds to PCNA, stimulates DNA excision repair, and initiates G2 cell cycle arrest. Human GADD45 alpha is 165 amino acids (aa) in length. It is reported to dimerize with GADD45-beta and -gamma, and to homo-dimerize, -trimerize and -tetramerize via residues 33‑61 and 133‑165. There is one splice variant that shows a Ser substitution for aa 14‑49. GADD45 alpha is 55% aa identical to GADD45-beta and -gamma, and shares 94% aa identity with mouse GADD45 alpha.

Long Name

Growth Arrest and DNA Damage Gene alpha

Alternate Names

GADD45a

Entrez Gene IDs

1647 (Human); 13197 (Mouse); 25112 (Rat)

Gene Symbol

GADD45A

UniProt

P24522

Additional GADD45 alpha Products

Product Documents for Human GADD45 alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GADD45 alpha Antibody

For research use only

Related Research Areas

Adult Neurogenesis
Cancer Biomarkers

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