Human GATA-3 Alexa Fluor® 594-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human GATA-3 in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 634919
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human GATA-3
    Accession # P23771
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of GATA‑3 in Human PBMCs by Flow Cytometry. Th2-stimulated human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3791N) and either (A) Mouse Anti-Human GATA‑3 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC63301T) or (B) Mouse IgG2B Alexa Fluor 594 Isotype Control (Catalog # IC0041T). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GATA-3
GATA-3 is a 54-60 kDa member of the GATA family of transcription regulating factors. There are currently six mammalian members, each of which binds to a G-A-T-A motif found in gene promoters. Although GATA-3 is traditionally described as being a hematopoietic transcriptional regulator, it has been found in multiple cell types, both embryonic and adult. Postnatal cells reported to express GATA-3 include NK cells, ILCs, NKT cells, B cells, thymocytes (DN, CD4 SP, and CD8 SP) and T cells, plus keratinocytes, sympathetic neurons, renal distal convoluted tubule and mammary duct epithelium. Human GATA-3 is 443 amino acids (aa) in length. It contains two GAGA-type Zn finger domains (aa 263-287 and 317-341) and multiple phosphorylation sites. GATAs as a group are known to either activate, or repress, gene expression, maintain transcriptional activity, and regulate gene expression levels. Within this framework, GATA-3 is best known to drive naïve CD4+ T cells into a Th2 phenotype, induce Th2 proliferation, and inhibit Th1 cell development via T-bet repression. Other effects attributed to GATA-33 include the promotion of Th9 and Treg formation, and the inhibition of Th1, TH17 and B cell development. Over aa 135-258, human and mouse GATA-3 share 94% aa sequence identity.
  • Entrez Gene IDs:
    2625 (Human); 14462 (Mouse)
  • Alternate Names:
    131320; GATA binding protein 3; GATA3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC0041T
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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