Detection of GATA‑3 in Human PBMCs by Flow Cytometry.
Th2-stimulated human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3791N) and either (A) Mouse Anti-Human GATA‑3 APC‑conjugated Monoclonal Antibody (Catalog # IC63301A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
GATA-3 is a 54-60 kDa member of the GATA family of
transcription regulating factors. There
are currently six mammalian members, each of which binds to a G-A-T-A motif
found in gene promoters. Although GATA-3
is traditionally described as being a hematopoietic transcriptional regulator,
it has been found in multiple cell types, both embryonic and adult. Postnatal cells reported to express GATA-3
include NK cells, ILCs, NKT cells, B cells, thymocytes (DN, CD4 SP, and CD8 SP)
and T cells, plus keratinocytes, sympathetic neurons, renal distal convoluted
tubule and mammary duct epithelium.
Human GATA-3 is 443 amino acids (aa) in length. It contains two GAGA-type Zn finger domains
(aa 263-287 and 317-341) and multiple phosphorylation sites. GATAs as a group are known to either activate,
or repress, gene expression, maintain transcriptional activity, and regulate
gene expression levels. Within this
framework, GATA-3 is best known to drive naïve CD4+ T cells into a Th2
phenotype, induce Th2 proliferation, and inhibit Th1 cell development via T-bet
repression. Other effects attributed to
GATA-33 include the promotion of Th9 and Treg formation, and the inhibition of
Th1, TH17 and B cell development. Over
aa 135-258, human and mouse GATA-3 share 94% aa sequence identity.
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