Intracellular Staining by Flow Cytometry
|Detection of GATA‑3 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human GATA‑3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC26051F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.
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