Human GATA-3 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of GATA‑3 in MCF‑7 Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Reviews (1)

Human GATA-3 PE-conjugated Antibody Summary

Species Reactivity
Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human GATA-1, -2, -4, -5, or ‑6.
Monoclonal Mouse IgG1 Clone # 291106
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human GATA-3
Accession # P23771
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of GATA-3 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of GATA‑3 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human GATA-3 PE-conjugated Monoclonal Antibody (Catalog # IC26051P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.

Entrez Gene IDs
2625 (Human); 14462 (Mouse)
Alternate Names
131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3

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Citations for Human GATA-3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
    Authors: SF Costa, VO Gomes, MO Dos Santos, LM Melo, GL Venturin, JP Bragato, GT Rebech, C de Oliveir, BM Nascimento, G Gileno de, VM Felix de L
    PLoS Negl Trop Dis, 2020;14(1):e0008021.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes
    Authors: LM Melo, JP Bragato, GL Venturin, GT Rebech, SF Costa, LE Garcia, FL Lopes, FR Eugênio, PS Patto Dos, VMF de Lima
    PLoS ONE, 2019;14(12):e0226192.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human GATA-3 PE-conjugated Antibody
By Anonymous on 02/11/2019
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human