Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 291106
View all GATA-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771

Specificity

Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with rhGATA-1, -2, -4, -5, or -6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GATA‑3 Antibody

Detection of Human GATA-3 antibody by Western Blot.

Detection of Human GATA‑3 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GATA-3 full length (FL) at approximately 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
GATA‑3 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

GATA‑3 in MCF-7 Human Cell Line.

GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GATA‑3 Monoclonal Antibody (Catalog # MAB26051) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GATA‑3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.

Alternate Names

GATA3

Entrez Gene IDs

2625 (Human); 14462 (Mouse)

Gene Symbol

GATA3

UniProt

P23771

Additional GATA-3 Products

Product Documents for Human GATA‑3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑3 Antibody

For research use only

Citations for Human GATA‑3 Antibody

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Protocols

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