Human GATA-3 Antibody Summary
Accession # P23771
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GATA‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GATA-3 full length (FL) at approximately 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
GATA‑3 in MCF-7 Human Cell Line. GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GATA‑3 Monoclonal Antibody (Catalog # MAB26051) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.
Citations for Human GATA-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Liver group 2 innate lymphoid cells regulate blood glucose levels through IL-13 signaling and suppression of gluconeogenesis
Authors: M Fujimoto, M Yokoyama, M Kiuchi, H Hosokawa, A Nakayama, N Hashimoto, I Sakuma, H Nagano, K Yamagata, F Kudo, I Manabe, E Lee, R Hatano, A Onodera, K Hirahara, K Yokote, T Miki, T Nakayama, T Tanaka
Nature Communications, 2022;13(1):5408.
Sample Types: Cell Lysates
Cyclic regulation of T-Bet and GATA-3 in human endometrium.
Authors: Inman D, Kawana K, Schust D
Reprod Sci, 2008;15(1):83-90.
Sample Types: Whole Tissue
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