Human GATA-4 Antibody

(12 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human GATA-4 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human GATA-4
    Met27-Phe211
    Accession # P43694
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Chromatin Immunoprecipitation (ChIP)
    5 µg/5 x 106 cells
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human GATA‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GATA‑4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
Detection of GATA‑4-regulated Genes by Chromatin Immunoprecipitation. KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR.
Immunocytochemistry
GATA‑4 in Differentiated Human Embryonic Stem Cells. GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
GATA‑4 in Differentiated Human Embryonic Stem Cells. GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-4
GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.
  • Entrez Gene IDs:
    2626 (Human)
  • Alternate Names:
    GATA binding protein 4; GATA4; GATA-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017;19(1):20-35.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro
    PLoS ONE, 2016;11(11):e0165926.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC - Fresh
  3. Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
    Authors: Nagaoka M, Kobayashi M, Kawai C, Mallanna S, Duncan S
    PLoS ONE, 2015;10(8):e0136350.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  4. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates.
    Authors: Yoon, Se-Jin, Foley, Joseph W, Baker, Julie C
    Nat Commun, 2015;6(0):6546.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta.
    Authors: Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L
    J Virol, 2015;89(9):5134-47.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  6. GATA family members as inducers for cellular reprogramming to pluripotency.
    Authors: Shu, Jian, Zhang, Ke, Zhang, Minjie, Yao, Anzhi, Shao, Sida, Du, Fengxia, Yang, Caiyun, Chen, Wenhan, Wu, Chen, Yang, Weifeng, Sun, Yingli, Deng, Hongkui
    Cell Res, 2015;25(2):169-80.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  7. A thyroid hormone receptor/KLF9 axis in human hepatocytes and pluripotent stem cells.
    Authors: Cvoro A, Devito L, Milton F, Noli L, Zhang A, Filippi C, Sakai K, Suh J, H Sieglaff D, Dhawan A, Sakai T, Ilic D, Webb P
    Stem Cells, 2015;33(2):416-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
    Authors: Villa-Diaz L, Kim J, Lahann J, Krebsbach P
    Stem Cells Transl Med, 2014;3(12):1410-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo.
    Authors: van Berlo JH, Elrod JW, Aronow BJ
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(30):12331-6.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.
    Authors: Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL
    Nat. Cell Biol., 2008;10(7):837-48.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. The human mucin MUC4 is transcriptionally regulated by caudal-related homeobox, hepatocyte nuclear factors, forkhead box A, and GATA endodermal transcription factors in epithelial cancer cells.
    Authors: Jonckheere N, Vincent A, Perrais M, Ducourouble MP, Male AK, Aubert JP, Pigny P, Carraway KL, Freund JN, Renes IB, Van Seuningen I
    J. Biol. Chem., 2007;282(31):22638-50.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  12. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
    Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
    Biotechnol. Bioeng., 2007;97(6):1617-25.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 12 Citations
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Average Rating: 5 (Based on 2 reviews)

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We have 1 review tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Immunohistochemistry, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human GATA-4 Antibody AF2606
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Excellent
 IHC Anonymous 03/31/2017
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Image Details
Immunohistochemistry Human GATA-4 Antibody AF2606
Immunohistochemistry: Human GATA-4 Antibody [AF2606]

Summary

ApplicationImmunohistochemistry
Sample TestedPancreas tissue

Other Experimental Details

Other Experimental Details(A) Human pancreas, 200X (B) Mouse pancreas, Gata4 wild-type, 200X (C) Mouse pancreas, Gata4 knock-out, 200X.
Western Blot Human GATA-4 Antibody AF2606
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Excellent
 WB Mouse Anonymous 03/31/2017
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Western Blot Human GATA-4 Antibody AF2606
Western Blot: Human GATA-4 Antibody [AF2606]

Summary

ApplicationWestern Blot
Sample TestedPDAC Cell Lines,Ovary tissue
SpeciesMouse

Other Experimental Details

Other Experimental Details75ug of protein from PDAC cell lines and 50 ug of protein from mouse ovary were loaded and incubated overnight at 1:1000 dilution with Gata4 antibody (AF2606). HRP-conjugated rabbit Anti-Goat IgG Secondary Antibody Rabbit was used. Vinculin was used as a loading control.

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