Human GATA‑4 Antibody

R&D Systems | Catalog # AF2606

R&D Systems
Discontinued Product
AF2606 has been discontinued. View all GATA-4 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GATA-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694

Specificity

Detects human GATA-4 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GATA‑4 Antibody

Detection of Human GATA‑4 antibody by Western Blot.

Detection of Human GATA‑4 by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GATA-4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of GATA-4-regulated Genes antibody by Chromatin Immunoprecipitation.

Detection of GATA‑4-regulated Genes by Chromatin Immunoprecipitation.

KATO-III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA-4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human GATA-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Themucin4promoter was detected by standard PCR.

GATA-4 antibody in Differentiated Human Embryonic Stem Cells by Immunocytochemistry (ICC).

GATA‑4 in Differentiated Human Embryonic Stem Cells.

GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GATA-4 antibody in Differentiated Human Embryonic Stem Cells by Immunocytochemistry (ICC).

GATA‑4 in Differentiated Human Embryonic Stem Cells.

GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GATA‑4 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: KATO‑III human gastric carcinoma cell line chromatin, mucin4 promoter detected by standard PCR.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed differentiated human embryonic stem cells

Western Blot

1 µg/mL
Sample: Human heart tissue

Reviewed Applications

Read 3 reviews rated 4.3 using AF2606 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-4

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Alternate Names

GATA4

Entrez Gene IDs

2626 (Human)

Gene Symbol

GATA4

UniProt

P43694

Additional GATA-4 Products

Product Documents for Human GATA‑4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑4 Antibody

For research use only

Citations for Human GATA‑4 Antibody

Customer Reviews for Human GATA‑4 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human GATA-4 Antibody
    Name: Megan Larson
    Application: Immunohistochemistry
    Sample Tested: Pancreas tissue
    Species: Human and Mouse
    Verified Customer | Posted 03/31/2017
    (A) Human pancreas, 200X (B) Mouse pancreas, Gata4 wild-type, 200X (C) Mouse pancreas, Gata4 knock-out, 200X.
    Human GATA‑4 Antibody AF2606
  • Human GATA-4 Antibody
    Name: Megan Larson
    Application: Western Blot
    Sample Tested: PDAC Cell Lines and Ovary tissue
    Species: Mouse
    Verified Customer | Posted 03/31/2017
    75ug of protein from PDAC cell lines and 50 ug of protein from mouse ovary were loaded and incubated overnight at 1:1000 dilution with Gata4 antibody (AF2606). HRP-conjugated rabbit Anti-Goat IgG Secondary Antibody Rabbit was used. Vinculin was used as a loading control.
    Human GATA‑4 Antibody AF2606
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23658023
    Species: Human
    Verified Customer | Posted 01/07/2015

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Protocols

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