GDF-15 (Growth Differentiation Factor 15) is a dimeric TGF-beta superfamily cytokine that is highly expressed in placenta and brain. It promotes neuronal survival and regulates neuropeptide Y and pro-opiomelanocortin levels to suppress appetite. It is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis. GDF-15 also inhibits cartilage formation and the production of TNF-alpha production from LPS-stimulated macrophages.
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Human GDF-15 Quantikine ELISA Kit
R&D Systems | Catalog # DGD150
Quantikine Quickit version available - results in 90 minutes (QK957).
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Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL), Urine (10 µL)
Sensitivity
4.39 pg/mL
Assay Range
23.4-1500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human GDF-15 Quantikine ELISA Kit
The Quantikine Human GDF-15 immunoassay is a 3.5 hour solid phase ELISA designed to measure GDF-15 in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human GDF-15 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural GDF-15 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of naturally occurring GDF-15.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human GDF-15
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Sample Values
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
| Serum (n=35) | 565 | 337-1060 |
| EDTA Plasma (n=35) | 534 | 289-1096 |
| Heparin Plasma (n=35) | 507 | 278-1064 |
| Sample Type | Mean (ng/mL) | Range (ng/mL) |
| Urine (n=15) | 5.71 | 1.00-20.1 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 238 | 456 | 886 | 225 | 442 | 900 |
| Standard Deviation | 4.27 | 9.86 | 25.0 | 13.4 | 20.6 | 50.5 |
| CV% | 1.8 | 2.2 | 2.8 | 6.0 | 4.7 | 5.6 |
Recovery for Human GDF-15 Quantikine ELISA Kit
The recovery of GDF-15 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 94-106 |
| EDTA Plasma (n=4) | 97 | 85-108 |
| Heparin Plasma (n=4) | 96 | 86-108 |
| Serum (n=4) | 98 | 88-107 |
| Urine (n=4) | 102 | 88-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of GDF-15 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human GDF-15 Quantikine ELISA Kit
Human GDF-15 Quantikine ELISA Kit Linearity Competitor Comparison
Human GDF-15 Quantikine ELISA Kit Recovery Competitor Comparison
Human GDF-15 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GDF-15
Long Name
Growth Differentiation Factor 15
Alternate Names
GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta
Gene Symbol
GDF15
Additional GDF-15 Products
Product Documents for Human GDF-15 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human GDF-15 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human GDF-15 Quantikine ELISA Kit
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Sample Tested: SerumVerified Customer | Posted 03/21/2023
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Sample Tested: SerumVerified Customer | Posted 09/13/2020
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Sample Tested: Serum and PlasmaVerified Customer | Posted 03/14/2019
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Sample Tested: extravillous trophoblasts conditioned mediumVerified Customer | Posted 01/22/2017Used to measure GDF-15 concentration in first trimester extravillous trophoblasts.
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Protocols
View specific protocols for Human GDF-15 Quantikine ELISA Kit (DGD150):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human GDF-15 Quantikine ELISA Kit
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Q: Does the Human GDF-15 Quantikine ELISA Kit detect a specific GDF-15 variant?
A: The kit is designed to target wild-type Human GDF-15 protein (accession #Q99988). However, we are aware of publications that cite the use of this kit for measurement of other GDF-15 variants.