Human GDNF Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DGD00
Control Products Available
Ancillary Products Available
Human GDNF Standard Curve
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Product Details
FAQs
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Human GDNF Quantikine ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.696 pg/mL
Assay Range
4.7 - 300 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human GDNF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human GDNF Immunoassay is a 4.0 hour solid phase ELISA designed to measure human GDNF levels in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant human GDNF and antibodies raised against the recombinant protein. Results obtained for naturally occurring human GDNF showed linear curves that were parallel to the standard curves obtained using the Quantikine® Human GDNF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human GDNF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 30.1 92.2 183 29.7 88.2 178
Standard Deviation 0.727 1.38 3.4 1.82 5.16 10.3
CV% 2.4 1.5 1.9 6.1 5.8 5.9

Recovery

The recovery of human GDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 85-112
Cell Lysis Buffer (n=4) 99 93-107
EDTA Plasma (n=5) 81 65-91
Heparin Plasma (n=5) 84 69-95
Serum (n=5) 86 72-99

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human GDNF were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human GDNF Linearity

Data Examples

Human GDNF Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: GDNF

GDNF (Glial Cell Line-derived Neurotrophic Factor) is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. It is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF binding to GFR alpha 1 induces the recruitment of Ret, NCAM-1/CD56, various integrins, Syndecan-3, or N-Cadherin. GDNF-based therapies show promise in several neurodegenerative disorders, particularly Parkinson’s disease.

Long Name:
Glial Cell line-derived Growth Factor
Entrez Gene IDs:
2668 (Human); 25453 (Rat)
Alternate Names:
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3

FAQs

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