Human GDNF Quantikine ELISA Kit Summary
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Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human GDNF spiked to levels throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||102||85-112|
|Cell Lysis Buffer (n=4)||99||93-107|
|EDTA Plasma (n=5)||81||65-91|
|Heparin Plasma (n=5)||84||69-95|
Human GDNF Standard Curve
Preparation and Storage
GDNF (Glial Cell Line-derived Neurotrophic Factor) is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. It is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF binding to GFR alpha 1 induces the recruitment of Ret, NCAM-1/CD56, various integrins, Syndecan-3, or N-Cadherin. GDNF-based therapies show promise in several neurodegenerative disorders, particularly Parkinson’s disease.
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